Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis
The objective of this study was to investigate the effects of selenium yeast on the growth, histomorphology, antioxidant capacity and apoptosis of largemouth bass (Micropterus salmoide). The five iso-nitrogen and iso-lipid feeds were supplemented with selenium yeast levels of 0, 0.5, 1.0, 1.5 and 2....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000443 |
| _version_ | 1797862147311534080 |
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| author | Zhixiong Xu Junru Hu Yufan Zhang Lirong Bai |
| author_facet | Zhixiong Xu Junru Hu Yufan Zhang Lirong Bai |
| author_sort | Zhixiong Xu |
| collection | DOAJ |
| description | The objective of this study was to investigate the effects of selenium yeast on the growth, histomorphology, antioxidant capacity and apoptosis of largemouth bass (Micropterus salmoide). The five iso-nitrogen and iso-lipid feeds were supplemented with selenium yeast levels of 0, 0.5, 1.0, 1.5 and 2.0 mg/kg, respectively. The 525 largemouth bass (initial body weight: 11.48 ± 0.43 g) were equally divided into 15 buckets (three buckets per feed treatment) for a 67-day feeding experiment. The results showed that the addition of 1.5 and 2.0 mg/kg of selenium yeast significantly reduced weight gain rate, and specific growth rate (P < 0.05), disorganized the structure of liver cells and exacerbated the vacuolation of liver tissue in largemouth bass. The activities of glutathione peroxidase and superoxide dismutase in serum were significantly increased by 1.0 mg/kg selenium yeast (P < 0.05). After selenium yeast was supplemented, villus length and villus width were significantly increased (P < 0.05), NO contents and respiratory burst activities in hematocyte were not significantly changed (P > 0.05). With the addition of 1.5 mg/kg of selenium yeast, the relative expression of growth hormone 1, insulin-like growth factor-1, superoxide dismutase, catalase, Kelch-like ECH-associating protein 1, and nuclear factor erythroid 2 related factor 2 in hematocyte was significantly increased (P < 0.05). The significantly lowest percentage of apoptotic hematocyte was also seen in the 1.5 mg/kg selenium yeast addition group (P < 0.05). Overall, the supplementation of 1.5 mg/kg selenium yeast was not conducive to the growth and liver development of largemouth bass, but it could improve the antioxidant capacity of hematocyte and inhibit the apoptosis. It provided a theoretical basis for the enhancement of immune capacity during the farming of largemouth bass. |
| first_indexed | 2024-04-09T22:15:39Z |
| format | Article |
| id | doaj.art-ee09331099954fc9b9294f1815c0a4b3 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:15:39Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ee09331099954fc9b9294f1815c0a4b32023-03-23T04:36:26ZengElsevierAquaculture Reports2352-51342023-04-0129101505Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosisZhixiong Xu0Junru Hu1Yufan Zhang2Lirong Bai3Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs，Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Corresponding author.Beijing Alltech Biological Products Co., Ltd., Beijing 101499, ChinaGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, ChinaThe objective of this study was to investigate the effects of selenium yeast on the growth, histomorphology, antioxidant capacity and apoptosis of largemouth bass (Micropterus salmoide). The five iso-nitrogen and iso-lipid feeds were supplemented with selenium yeast levels of 0, 0.5, 1.0, 1.5 and 2.0 mg/kg, respectively. The 525 largemouth bass (initial body weight: 11.48 ± 0.43 g) were equally divided into 15 buckets (three buckets per feed treatment) for a 67-day feeding experiment. The results showed that the addition of 1.5 and 2.0 mg/kg of selenium yeast significantly reduced weight gain rate, and specific growth rate (P < 0.05), disorganized the structure of liver cells and exacerbated the vacuolation of liver tissue in largemouth bass. The activities of glutathione peroxidase and superoxide dismutase in serum were significantly increased by 1.0 mg/kg selenium yeast (P < 0.05). After selenium yeast was supplemented, villus length and villus width were significantly increased (P < 0.05), NO contents and respiratory burst activities in hematocyte were not significantly changed (P > 0.05). With the addition of 1.5 mg/kg of selenium yeast, the relative expression of growth hormone 1, insulin-like growth factor-1, superoxide dismutase, catalase, Kelch-like ECH-associating protein 1, and nuclear factor erythroid 2 related factor 2 in hematocyte was significantly increased (P < 0.05). The significantly lowest percentage of apoptotic hematocyte was also seen in the 1.5 mg/kg selenium yeast addition group (P < 0.05). Overall, the supplementation of 1.5 mg/kg selenium yeast was not conducive to the growth and liver development of largemouth bass, but it could improve the antioxidant capacity of hematocyte and inhibit the apoptosis. It provided a theoretical basis for the enhancement of immune capacity during the farming of largemouth bass.http://www.sciencedirect.com/science/article/pii/S2352513423000443Antioxidant abilityApoptosisGrowthHistopathologySelenium yeast |
| spellingShingle | Zhixiong Xu Junru Hu Yufan Zhang Lirong Bai Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis Aquaculture Reports Antioxidant ability Apoptosis Growth Histopathology Selenium yeast |
| title | Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis |
| title_full | Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis |
| title_fullStr | Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis |
| title_full_unstemmed | Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis |
| title_short | Evaluation of largemouth bass (Micropterus salmoide) fed selenium yeast diets: Growth, histopathology, antioxidant ability, and apoptosis |
| title_sort | evaluation of largemouth bass micropterus salmoide fed selenium yeast diets growth histopathology antioxidant ability and apoptosis |
| topic | Antioxidant ability Apoptosis Growth Histopathology Selenium yeast |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000443 |
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