Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis.
The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples ob...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0241164 |
| _version_ | 1819042512211279872 |
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| author | Victoria Indenbaum Ravit Koren Shiri Katz-Likvornik Mayan Yitzchaki Osnat Halpern Gili Regev-Yochay Carmit Cohen Asaf Biber Tali Feferman Noy Cohen Saban Roni Dhan Tal Levin Yael Gozlan Merav Weil Orna Mor Michal Mandelboim Danit Sofer Ella Mendelson Yaniv Lustig |
| author_facet | Victoria Indenbaum Ravit Koren Shiri Katz-Likvornik Mayan Yitzchaki Osnat Halpern Gili Regev-Yochay Carmit Cohen Asaf Biber Tali Feferman Noy Cohen Saban Roni Dhan Tal Levin Yael Gozlan Merav Weil Orna Mor Michal Mandelboim Danit Sofer Ella Mendelson Yaniv Lustig |
| author_sort | Victoria Indenbaum |
| collection | DOAJ |
| description | The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4-7 samples each ranging between 1-92 days post first positive PCR were tested by an "in house" ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1-7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35-50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2. |
| first_indexed | 2024-12-21T09:42:04Z |
| format | Article |
| id | doaj.art-ee099ea138f74deea8255cb058d0f5a3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:42:04Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ee099ea138f74deea8255cb058d0f5a32022-12-21T19:08:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011511e024116410.1371/journal.pone.0241164Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis.Victoria IndenbaumRavit KorenShiri Katz-LikvornikMayan YitzchakiOsnat HalpernGili Regev-YochayCarmit CohenAsaf BiberTali FefermanNoy Cohen SabanRoni DhanTal LevinYael GozlanMerav WeilOrna MorMichal MandelboimDanit SoferElla MendelsonYaniv LustigThe COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4-7 samples each ranging between 1-92 days post first positive PCR were tested by an "in house" ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1-7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35-50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2.https://doi.org/10.1371/journal.pone.0241164 |
| spellingShingle | Victoria Indenbaum Ravit Koren Shiri Katz-Likvornik Mayan Yitzchaki Osnat Halpern Gili Regev-Yochay Carmit Cohen Asaf Biber Tali Feferman Noy Cohen Saban Roni Dhan Tal Levin Yael Gozlan Merav Weil Orna Mor Michal Mandelboim Danit Sofer Ella Mendelson Yaniv Lustig Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. PLoS ONE |
| title | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. |
| title_full | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. |
| title_fullStr | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. |
| title_full_unstemmed | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. |
| title_short | Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. |
| title_sort | testing igg antibodies against the rbd of sars cov 2 is sufficient and necessary for covid 19 diagnosis |
| url | https://doi.org/10.1371/journal.pone.0241164 |
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