A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro
Osteosarcoma is an aggressive malignant neoplasm, and it is of great significance to the fabrication and investigation of the anti-tumor mechanism of nanomedicine in the treatment of osteosarcoma. Herein, a cinnamaldehyde polymeric prodrug micelle with pH-sensitive charge-conversion ability (mPEG-&l...
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MDPI AG
2023-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1524 |
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| author | Jiapeng Deng Qichang Wang Huihui Xu Guoqing Li Su Liu Yixiao Chen Fei Yu Weiqiang Yan Hui Zeng Peng Liu |
| author_facet | Jiapeng Deng Qichang Wang Huihui Xu Guoqing Li Su Liu Yixiao Chen Fei Yu Weiqiang Yan Hui Zeng Peng Liu |
| author_sort | Jiapeng Deng |
| collection | DOAJ |
| description | Osteosarcoma is an aggressive malignant neoplasm, and it is of great significance to the fabrication and investigation of the anti-tumor mechanism of nanomedicine in the treatment of osteosarcoma. Herein, a cinnamaldehyde polymeric prodrug micelle with pH-sensitive charge-conversion ability (mPEG-<i>b</i>-P(C7-<i>co</i>-CA)) was fabricated, and the anti-osteosarcoma mechanism of mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelle was investigated. mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles were prepared by self-assembly method, and their diameter was 227 nm. mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles could regulate the cell cycle and inhibit the proliferation of 143B cells, which was demonstrated by flow cytometry analysis, CCK-8 assay and 5-Ethynyl-2′-deoxyuridine (EdU) staining. The wound-healing assay and transwell assay showed that mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles effectively inhibited the migration and invasion of 143B cells. It was proven that mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles downregulated the levels of proliferation and apoptosis-related proteins and affected osteosarcoma migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT). In addition, mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles can also inhibit the transcriptional activity of the PI3K/Akt signaling pathway. Therefore, these findings provide new evidence for the pharmacological effects of mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles. |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:45:16Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ee0c2dcf996841628e1dbf37f7c4843d2023-11-18T09:24:34ZengMDPI AGBiomedicines2227-90592023-05-01116152410.3390/biomedicines11061524A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In VitroJiapeng Deng0Qichang Wang1Huihui Xu2Guoqing Li3Su Liu4Yixiao Chen5Fei Yu6Weiqiang Yan7Hui Zeng8Peng Liu9National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaDepartment of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaOsteosarcoma is an aggressive malignant neoplasm, and it is of great significance to the fabrication and investigation of the anti-tumor mechanism of nanomedicine in the treatment of osteosarcoma. Herein, a cinnamaldehyde polymeric prodrug micelle with pH-sensitive charge-conversion ability (mPEG-<i>b</i>-P(C7-<i>co</i>-CA)) was fabricated, and the anti-osteosarcoma mechanism of mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelle was investigated. mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles were prepared by self-assembly method, and their diameter was 227 nm. mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles could regulate the cell cycle and inhibit the proliferation of 143B cells, which was demonstrated by flow cytometry analysis, CCK-8 assay and 5-Ethynyl-2′-deoxyuridine (EdU) staining. The wound-healing assay and transwell assay showed that mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles effectively inhibited the migration and invasion of 143B cells. It was proven that mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles downregulated the levels of proliferation and apoptosis-related proteins and affected osteosarcoma migration and invasion by inhibiting the epithelial-mesenchymal transition (EMT). In addition, mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles can also inhibit the transcriptional activity of the PI3K/Akt signaling pathway. Therefore, these findings provide new evidence for the pharmacological effects of mPEG-<i>b</i>-P(C7-<i>co</i>-CA) micelles.https://www.mdpi.com/2227-9059/11/6/1524cinnamaldehyde prodrugosteosarcoma targetingPI3K/Aktmicelles |
| spellingShingle | Jiapeng Deng Qichang Wang Huihui Xu Guoqing Li Su Liu Yixiao Chen Fei Yu Weiqiang Yan Hui Zeng Peng Liu A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro Biomedicines cinnamaldehyde prodrug osteosarcoma targeting PI3K/Akt micelles |
| title | A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro |
| title_full | A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro |
| title_fullStr | A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro |
| title_full_unstemmed | A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro |
| title_short | A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro |
| title_sort | systematic study of anti osteosarcoma mechanism of ph sensitive charge conversion cinnamaldehyde polymeric prodrug micelles in vitro |
| topic | cinnamaldehyde prodrug osteosarcoma targeting PI3K/Akt micelles |
| url | https://www.mdpi.com/2227-9059/11/6/1524 |
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