Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer
To identify cancer/testis (CT) antigens and immunogenic proteins, immunoscreening of testicular and small-cell lung cancer cell line NCI-H889 cDNA libraries was performed using serum obtained from a small-cell lung cancer (SCLC) patient. We obtained 113 positive cDNA clones comprised of 74 different...
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MDPI AG
2021-05-01
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| Series: | Current Oncology |
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| Online Access: | https://www.mdpi.com/1718-7729/28/3/179 |
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| author | Ye-Rin Kim Ki-Uk Kim Jung-Hee Lee Deok-Won Kim Jae-Heun Chung Yeong-Dae Kim Dong-Hoon Shin Min-Ki Lee Yong-Il Shin Sang-Yull Lee |
| author_facet | Ye-Rin Kim Ki-Uk Kim Jung-Hee Lee Deok-Won Kim Jae-Heun Chung Yeong-Dae Kim Dong-Hoon Shin Min-Ki Lee Yong-Il Shin Sang-Yull Lee |
| author_sort | Ye-Rin Kim |
| collection | DOAJ |
| description | To identify cancer/testis (CT) antigens and immunogenic proteins, immunoscreening of testicular and small-cell lung cancer cell line NCI-H889 cDNA libraries was performed using serum obtained from a small-cell lung cancer (SCLC) patient. We obtained 113 positive cDNA clones comprised of 74 different genes, designated KP-SCLC-1 through KP-SCLC-74. Of these genes, 59 genes were found to be related to cancers by EMBASE analysis. Three of these antigens, including KP-SCLC-29 (NOL4), KP-SCLC-59 (CCDC83), and KP-SCLC-69 (KIF20B), were CT antigens. RT-PCR and western blot analysis showed that NOL4 was frequently present in small-cell lung cancer cell lines (8/9, 8/9). In addition, NOL4 mRNA was weakly, or at a low frequency, or not detected in various cancer cell lines. Our results reveal that NOL4 was expressed at protein levels in small-cell lung cancer tissues (10/10) but not detected in lung adenocarcinoma and squamous cell carcinoma by immunohistochemical analysis. Serological response to NOL4 was also evaluated by western blot assay using NOL4 recombinant protein. A humoral response against NOL4 proteins was detected in 75% (33/44) of small-cell lung cancer patients and in 65% (13/20) of healthy donors by a serological western blot assay. These data suggest that NOL4 is a specific target that may be useful for diagnosis and immunotherapy in SCLC. |
| first_indexed | 2024-03-10T08:01:33Z |
| format | Article |
| id | doaj.art-ee0c40e2d2e44e008ebc5b35fb49339e |
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| issn | 1198-0052 1718-7729 |
| language | English |
| last_indexed | 2024-03-10T08:01:33Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | Current Oncology |
| spelling | doaj.art-ee0c40e2d2e44e008ebc5b35fb49339e2023-11-22T11:28:30ZengMDPI AGCurrent Oncology1198-00521718-77292021-05-012831927193710.3390/curroncol28030179Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung CancerYe-Rin Kim0Ki-Uk Kim1Jung-Hee Lee2Deok-Won Kim3Jae-Heun Chung4Yeong-Dae Kim5Dong-Hoon Shin6Min-Ki Lee7Yong-Il Shin8Sang-Yull Lee9Department of Biochemistry, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 50612, KoreaDepartment of Internal Medicine, Pusan National University Hospital, 1-10 Ami-dong, Seo-gu, Busan 49241, KoreaDepartment of Pathology, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 50612, KoreaDepartment of Biochemistry, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 50612, KoreaDepartment of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan 50612, KoreaDepartment of Thoracic Surgery, Pusan National University Hospital, 1-10 Ami-dong, Seo-gu, Busan 49241, KoreaDepartment of Pathology, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 50612, KoreaDepartment of Internal Medicine, Pusan National University Hospital, 1-10 Ami-dong, Seo-gu, Busan 49241, KoreaResearch Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, KoreaDepartment of Biochemistry, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 50612, KoreaTo identify cancer/testis (CT) antigens and immunogenic proteins, immunoscreening of testicular and small-cell lung cancer cell line NCI-H889 cDNA libraries was performed using serum obtained from a small-cell lung cancer (SCLC) patient. We obtained 113 positive cDNA clones comprised of 74 different genes, designated KP-SCLC-1 through KP-SCLC-74. Of these genes, 59 genes were found to be related to cancers by EMBASE analysis. Three of these antigens, including KP-SCLC-29 (NOL4), KP-SCLC-59 (CCDC83), and KP-SCLC-69 (KIF20B), were CT antigens. RT-PCR and western blot analysis showed that NOL4 was frequently present in small-cell lung cancer cell lines (8/9, 8/9). In addition, NOL4 mRNA was weakly, or at a low frequency, or not detected in various cancer cell lines. Our results reveal that NOL4 was expressed at protein levels in small-cell lung cancer tissues (10/10) but not detected in lung adenocarcinoma and squamous cell carcinoma by immunohistochemical analysis. Serological response to NOL4 was also evaluated by western blot assay using NOL4 recombinant protein. A humoral response against NOL4 proteins was detected in 75% (33/44) of small-cell lung cancer patients and in 65% (13/20) of healthy donors by a serological western blot assay. These data suggest that NOL4 is a specific target that may be useful for diagnosis and immunotherapy in SCLC.https://www.mdpi.com/1718-7729/28/3/179KP-SCLC-29NOL4cancer/testis antigensmall-cell lung cancerSEREX |
| spellingShingle | Ye-Rin Kim Ki-Uk Kim Jung-Hee Lee Deok-Won Kim Jae-Heun Chung Yeong-Dae Kim Dong-Hoon Shin Min-Ki Lee Yong-Il Shin Sang-Yull Lee Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer Current Oncology KP-SCLC-29 NOL4 cancer/testis antigen small-cell lung cancer SEREX |
| title | Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer |
| title_full | Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer |
| title_fullStr | Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer |
| title_full_unstemmed | Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer |
| title_short | Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer |
| title_sort | cancer testis antigen nol4 is an immunogenic antigen specifically expressed in small cell lung cancer |
| topic | KP-SCLC-29 NOL4 cancer/testis antigen small-cell lung cancer SEREX |
| url | https://www.mdpi.com/1718-7729/28/3/179 |
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