Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants
Genome editing technology has become one of the hottest research areas in recent years. Among diverse genome editing tools, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins system (CRISPR/Cas system) has exhibited the obvious advantages of specificity, simplic...
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MDPI AG
2022-09-01
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| author | Xiao Ding Lu Yu Luo Chen Yujie Li Jinlun Zhang Hanyan Sheng Zhengwei Ren Yunlong Li Xiaohan Yu Shuangxia Jin Jinglin Cao |
| author_facet | Xiao Ding Lu Yu Luo Chen Yujie Li Jinlun Zhang Hanyan Sheng Zhengwei Ren Yunlong Li Xiaohan Yu Shuangxia Jin Jinglin Cao |
| author_sort | Xiao Ding |
| collection | DOAJ |
| description | Genome editing technology has become one of the hottest research areas in recent years. Among diverse genome editing tools, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins system (CRISPR/Cas system) has exhibited the obvious advantages of specificity, simplicity, and flexibility over any previous genome editing system. In addition, the emergence of Cas9 mutants, such as dCas9 (dead Cas9), which lost its endonuclease activity but maintains DNA recognition activity with the guide RNA, provides powerful genetic manipulation tools. In particular, combining the dCas9 protein and transcriptional activator to achieve specific regulation of gene expression has made important contributions to biotechnology in medical research as well as agriculture. CRISPR/dCas9 activation (CRISPRa) can increase the transcription of endogenous genes. Overexpression of foreign genes by traditional transgenic technology in plant cells is the routine method to verify gene function by elevating genes transcription. One of the main limitations of the overexpression is the vector capacity constraint that makes it difficult to express multiple genes using the typical Ti plasmid vectors from Agrobacterium. The CRISPRa system can overcome these limitations of the traditional gene overexpression method and achieve multiple gene activation by simply designating several guide RNAs in one vector. This review summarizes the latest progress based on the development of CRISPRa systems, including SunTag, dCas9-VPR, dCas9-TV, scRNA, SAM, and CRISPR-Act and their applications in plants. Furthermore, limitations, challenges of current CRISPRa systems and future prospective applications are also discussed. |
| first_indexed | 2024-03-09T21:54:18Z |
| format | Article |
| id | doaj.art-ee0c789d26384aec9a358e5cbf36e45c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T21:54:18Z |
| publishDate | 2022-09-01 |
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| series | Cells |
| spelling | doaj.art-ee0c789d26384aec9a358e5cbf36e45c2023-11-23T20:01:45ZengMDPI AGCells2073-44092022-09-011119304510.3390/cells11193045Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in PlantsXiao Ding0Lu Yu1Luo Chen2Yujie Li3Jinlun Zhang4Hanyan Sheng5Zhengwei Ren6Yunlong Li7Xiaohan Yu8Shuangxia Jin9Jinglin Cao10Institute of Cotton, Shanxi Agricultural University, Yuncheng 044000, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaTobacco Research Institute of Hubei Province, Wuhan 430030, ChinaGenome editing technology has become one of the hottest research areas in recent years. Among diverse genome editing tools, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins system (CRISPR/Cas system) has exhibited the obvious advantages of specificity, simplicity, and flexibility over any previous genome editing system. In addition, the emergence of Cas9 mutants, such as dCas9 (dead Cas9), which lost its endonuclease activity but maintains DNA recognition activity with the guide RNA, provides powerful genetic manipulation tools. In particular, combining the dCas9 protein and transcriptional activator to achieve specific regulation of gene expression has made important contributions to biotechnology in medical research as well as agriculture. CRISPR/dCas9 activation (CRISPRa) can increase the transcription of endogenous genes. Overexpression of foreign genes by traditional transgenic technology in plant cells is the routine method to verify gene function by elevating genes transcription. One of the main limitations of the overexpression is the vector capacity constraint that makes it difficult to express multiple genes using the typical Ti plasmid vectors from Agrobacterium. The CRISPRa system can overcome these limitations of the traditional gene overexpression method and achieve multiple gene activation by simply designating several guide RNAs in one vector. This review summarizes the latest progress based on the development of CRISPRa systems, including SunTag, dCas9-VPR, dCas9-TV, scRNA, SAM, and CRISPR-Act and their applications in plants. Furthermore, limitations, challenges of current CRISPRa systems and future prospective applications are also discussed.https://www.mdpi.com/2073-4409/11/19/3045CRISPRaCRISPR/CasdCas9genome editingtranscription activation |
| spellingShingle | Xiao Ding Lu Yu Luo Chen Yujie Li Jinlun Zhang Hanyan Sheng Zhengwei Ren Yunlong Li Xiaohan Yu Shuangxia Jin Jinglin Cao Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants Cells CRISPRa CRISPR/Cas dCas9 genome editing transcription activation |
| title | Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants |
| title_full | Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants |
| title_fullStr | Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants |
| title_full_unstemmed | Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants |
| title_short | Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants |
| title_sort | recent progress and future prospect of crispr cas derived transcription activation crispra system in plants |
| topic | CRISPRa CRISPR/Cas dCas9 genome editing transcription activation |
| url | https://www.mdpi.com/2073-4409/11/19/3045 |
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