The gut microbiota and metabolite profiles are altered in patients with spinal cord injury
Abstract Background Metabolites secreted by the gut microbiota may play an essential role in microbiota–gut–central nervous system crosstalk. In this study, we explored the changes occurring in the gut microbiota and their metabolites in patients with spinal cord injury (SCI) and analyzed the correl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-023-01014-0 |
| _version_ | 1827982352895180800 |
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| author | Ganggang Kong Wenwu Zhang Siyun Zhang Jiewen Chen kejun He Changming Zhang Xi Yuan Baoshu Xie |
| author_facet | Ganggang Kong Wenwu Zhang Siyun Zhang Jiewen Chen kejun He Changming Zhang Xi Yuan Baoshu Xie |
| author_sort | Ganggang Kong |
| collection | DOAJ |
| description | Abstract Background Metabolites secreted by the gut microbiota may play an essential role in microbiota–gut–central nervous system crosstalk. In this study, we explored the changes occurring in the gut microbiota and their metabolites in patients with spinal cord injury (SCI) and analyzed the correlations among them. Methods The structure and composition of the gut microbiota derived from fecal samples collected from patients with SCI (n = 11) and matched control individuals (n = 10) were evaluated using 16S rRNA gene sequencing. Additionally, an untargeted metabolomics approach was used to compare the serum metabolite profiles of both groups. Meanwhile, the association among serum metabolites, the gut microbiota, and clinical parameters (including injury duration and neurological grade) was also analyzed. Finally, metabolites with the potential for use in the treatment of SCI were identified based on the differential metabolite abundance analysis. Results The composition of the gut microbiota was different between patients with SCI and healthy controls. At the genus level, compared with the control group, the abundance of UBA1819, Anaerostignum, Eggerthella, and Enterococcus was significantly increased in the SCI group, whereas that of Faecalibacterium, Blautia, Escherichia–Shigella, Agathobacter, Collinsella, Dorea, Ruminococcus, Fusicatenibacter, and Eubacterium was decreased. Forty-one named metabolites displayed significant differential abundance between SCI patients and healthy controls, including 18 that were upregulated and 23 that were downregulated. Correlation analysis further indicated that the variation in gut microbiota abundance was associated with changes in serum metabolite levels, suggesting that gut dysbiosis is an important cause of metabolic disorders in SCI. Finally, gut dysbiosis and serum metabolite dysregulation was found to be associated with injury duration and severity of motor dysfunction after SCI. Conclusions We present a comprehensive landscape of the gut microbiota and metabolite profiles in patients with SCI and provide evidence that their interaction plays a role in the pathogenesis of SCI. Furthermore, our findings suggested that uridine, hypoxanthine, PC(18:2/0:0), and kojic acid may be important therapeutic targets for the treatment of this condition. |
| first_indexed | 2024-04-09T22:32:19Z |
| format | Article |
| id | doaj.art-ee0f889410234d35b6cbb1397fec3914 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-04-09T22:32:19Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-ee0f889410234d35b6cbb1397fec39142023-03-22T12:42:06ZengBMCMolecular Brain1756-66062023-02-0116111410.1186/s13041-023-01014-0The gut microbiota and metabolite profiles are altered in patients with spinal cord injuryGanggang Kong0Wenwu Zhang1Siyun Zhang2Jiewen Chen3kejun He4Changming Zhang5Xi Yuan6Baoshu Xie7Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, Department of Spinal Surgery, The First Affiliated Hospital of Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Orthopedics and Traumatology, Department of Spinal Surgery, The First Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Rehabilitation Medicine, The First Affiliated Hospital of Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Orthopedics and Traumatology, Department of Spinal Surgery, The First Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Anesthesiology, Bazhong Central HospitalDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-Sen UniversityAbstract Background Metabolites secreted by the gut microbiota may play an essential role in microbiota–gut–central nervous system crosstalk. In this study, we explored the changes occurring in the gut microbiota and their metabolites in patients with spinal cord injury (SCI) and analyzed the correlations among them. Methods The structure and composition of the gut microbiota derived from fecal samples collected from patients with SCI (n = 11) and matched control individuals (n = 10) were evaluated using 16S rRNA gene sequencing. Additionally, an untargeted metabolomics approach was used to compare the serum metabolite profiles of both groups. Meanwhile, the association among serum metabolites, the gut microbiota, and clinical parameters (including injury duration and neurological grade) was also analyzed. Finally, metabolites with the potential for use in the treatment of SCI were identified based on the differential metabolite abundance analysis. Results The composition of the gut microbiota was different between patients with SCI and healthy controls. At the genus level, compared with the control group, the abundance of UBA1819, Anaerostignum, Eggerthella, and Enterococcus was significantly increased in the SCI group, whereas that of Faecalibacterium, Blautia, Escherichia–Shigella, Agathobacter, Collinsella, Dorea, Ruminococcus, Fusicatenibacter, and Eubacterium was decreased. Forty-one named metabolites displayed significant differential abundance between SCI patients and healthy controls, including 18 that were upregulated and 23 that were downregulated. Correlation analysis further indicated that the variation in gut microbiota abundance was associated with changes in serum metabolite levels, suggesting that gut dysbiosis is an important cause of metabolic disorders in SCI. Finally, gut dysbiosis and serum metabolite dysregulation was found to be associated with injury duration and severity of motor dysfunction after SCI. Conclusions We present a comprehensive landscape of the gut microbiota and metabolite profiles in patients with SCI and provide evidence that their interaction plays a role in the pathogenesis of SCI. Furthermore, our findings suggested that uridine, hypoxanthine, PC(18:2/0:0), and kojic acid may be important therapeutic targets for the treatment of this condition.https://doi.org/10.1186/s13041-023-01014-0Spinal cord injuryGut microbiota16S rRNA gene sequencingUntargeted metabolomics |
| spellingShingle | Ganggang Kong Wenwu Zhang Siyun Zhang Jiewen Chen kejun He Changming Zhang Xi Yuan Baoshu Xie The gut microbiota and metabolite profiles are altered in patients with spinal cord injury Molecular Brain Spinal cord injury Gut microbiota 16S rRNA gene sequencing Untargeted metabolomics |
| title | The gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| title_full | The gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| title_fullStr | The gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| title_full_unstemmed | The gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| title_short | The gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| title_sort | gut microbiota and metabolite profiles are altered in patients with spinal cord injury |
| topic | Spinal cord injury Gut microbiota 16S rRNA gene sequencing Untargeted metabolomics |
| url | https://doi.org/10.1186/s13041-023-01014-0 |
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