Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density
Increased life expectancy in industrialized countries is causing an increased incidence of osteoporosis and the need for bioactive bone implants. The integration of implants can be improved physically, but mainly by chemical modifications of the material surface. It was recognized that amino-group-c...
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MDPI AG
2023-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/18/6505 |
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| author | Susanne Seemann Manuela Dubs Dirk Koczan Hernando S. Salapare Arnaud Ponche Laurent Pieuchot Tatiana Petithory Annika Wartenberg Susanne Staehlke Matthias Schnabelrauch Karine Anselme J. Barbara Nebe |
| author_facet | Susanne Seemann Manuela Dubs Dirk Koczan Hernando S. Salapare Arnaud Ponche Laurent Pieuchot Tatiana Petithory Annika Wartenberg Susanne Staehlke Matthias Schnabelrauch Karine Anselme J. Barbara Nebe |
| author_sort | Susanne Seemann |
| collection | DOAJ |
| description | Increased life expectancy in industrialized countries is causing an increased incidence of osteoporosis and the need for bioactive bone implants. The integration of implants can be improved physically, but mainly by chemical modifications of the material surface. It was recognized that amino-group-containing coatings improved cell attachment and intracellular signaling. The aim of this study was to determine the role of the amino group density in this positive cell behavior by developing controlled amino-rich nanolayers. This work used covalent grafting of polymer-based nanocoatings with different amino group densities. Titanium coated with the positively-charged trimethoxysilylpropyl modified poly(ethyleneimine) (Ti-TMS-PEI), which mostly improved cell area after 30 min, possessed the highest amino group density with an N/C of 32%. Interestingly, changes in adhesion-related genes on Ti-TMS-PEI could be seen after 4 h. The mRNA microarray data showed a premature transition of the MG-63 cells into the beginning differentiation phase after 24 h indicating Ti-TMS-PEI as a supportive factor for osseointegration. This amino-rich nanolayer also induced higher bovine serum albumin protein adsorption and caused the cells to migrate slower on the surface after a more extended period of cell settlement as an indication of a better surface anchorage. In conclusion, the cell spreading on amine-based nanocoatings correlated well with the amino group density (N/C). |
| first_indexed | 2024-03-10T22:25:38Z |
| format | Article |
| id | doaj.art-ee12535e5f80463483fc018f02b7d476 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T22:25:38Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ee12535e5f80463483fc018f02b7d4762023-11-19T12:08:20ZengMDPI AGMolecules1420-30492023-09-012818650510.3390/molecules28186505Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group DensitySusanne Seemann0Manuela Dubs1Dirk Koczan2Hernando S. Salapare3Arnaud Ponche4Laurent Pieuchot5Tatiana Petithory6Annika Wartenberg7Susanne Staehlke8Matthias Schnabelrauch9Karine Anselme10J. Barbara Nebe11Institute for Cell Biology, Rostock University Medical Center, 18057 Rostock, GermanyDepartment of Biomaterials, INNOVENT e.V., 07745 Jena, GermanyDepartment of Immunology, Rostock University Medical Center, 18057 Rostock, GermanyInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS, Université de Haute-Alsace, UMR 7361, 68100 Mulhouse, FranceInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS, Université de Haute-Alsace, UMR 7361, 68100 Mulhouse, FranceInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS, Université de Haute-Alsace, UMR 7361, 68100 Mulhouse, FranceInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS, Université de Haute-Alsace, UMR 7361, 68100 Mulhouse, FranceDepartment of Biomaterials, INNOVENT e.V., 07745 Jena, GermanyInstitute for Cell Biology, Rostock University Medical Center, 18057 Rostock, GermanyDepartment of Biomaterials, INNOVENT e.V., 07745 Jena, GermanyInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS, Université de Haute-Alsace, UMR 7361, 68100 Mulhouse, FranceInstitute for Cell Biology, Rostock University Medical Center, 18057 Rostock, GermanyIncreased life expectancy in industrialized countries is causing an increased incidence of osteoporosis and the need for bioactive bone implants. The integration of implants can be improved physically, but mainly by chemical modifications of the material surface. It was recognized that amino-group-containing coatings improved cell attachment and intracellular signaling. The aim of this study was to determine the role of the amino group density in this positive cell behavior by developing controlled amino-rich nanolayers. This work used covalent grafting of polymer-based nanocoatings with different amino group densities. Titanium coated with the positively-charged trimethoxysilylpropyl modified poly(ethyleneimine) (Ti-TMS-PEI), which mostly improved cell area after 30 min, possessed the highest amino group density with an N/C of 32%. Interestingly, changes in adhesion-related genes on Ti-TMS-PEI could be seen after 4 h. The mRNA microarray data showed a premature transition of the MG-63 cells into the beginning differentiation phase after 24 h indicating Ti-TMS-PEI as a supportive factor for osseointegration. This amino-rich nanolayer also induced higher bovine serum albumin protein adsorption and caused the cells to migrate slower on the surface after a more extended period of cell settlement as an indication of a better surface anchorage. In conclusion, the cell spreading on amine-based nanocoatings correlated well with the amino group density (N/C).https://www.mdpi.com/1420-3049/28/18/6505surface modificationamino coatingpolymermaterial characterizationsurface chargewettability |
| spellingShingle | Susanne Seemann Manuela Dubs Dirk Koczan Hernando S. Salapare Arnaud Ponche Laurent Pieuchot Tatiana Petithory Annika Wartenberg Susanne Staehlke Matthias Schnabelrauch Karine Anselme J. Barbara Nebe Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density Molecules surface modification amino coating polymer material characterization surface charge wettability |
| title | Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density |
| title_full | Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density |
| title_fullStr | Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density |
| title_full_unstemmed | Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density |
| title_short | Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density |
| title_sort | response of osteoblasts on amine based nanocoatings correlates with the amino group density |
| topic | surface modification amino coating polymer material characterization surface charge wettability |
| url | https://www.mdpi.com/1420-3049/28/18/6505 |
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