Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers
The in vitro diagnostics of cancer are not represented well yet, but the need for early-stage detection is undeniable. In recent decades, surface-enhanced Raman spectroscopy (SERS) has emerged as an efficient, adaptable, and unique technique for the detection of cancer molecules in their early stage...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7821 |
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| author | Anamika Pandey Subhankar Sarkar Sumit Kumar Pandey Anchal Srivastava |
| author_facet | Anamika Pandey Subhankar Sarkar Sumit Kumar Pandey Anchal Srivastava |
| author_sort | Anamika Pandey |
| collection | DOAJ |
| description | The in vitro diagnostics of cancer are not represented well yet, but the need for early-stage detection is undeniable. In recent decades, surface-enhanced Raman spectroscopy (SERS) has emerged as an efficient, adaptable, and unique technique for the detection of cancer molecules in their early stages. Herein, we demonstrate an opto-plasmonic hybrid structure for sensitive detection of the prostate cancer biomarker sarcosine using silica nanospheres coated silver nano-islands as a facile and efficient SERS active substrate. The SERS active platform has been developed via thin (5–15 nm) deposition of silver islands using a simple and cost-effective Radio Frequency (RF) sputtering technique followed by the synthesis and decoration of silica nanospheres (~500 nm) synthesized via Stober’s method. It is anticipated that the coupling of Whispering Gallery Modes and photonic nano-jets in SiO<sub>2</sub> nanospheres induce Localized Surface Plasmon Resonance (LSPR) in Ag nano-islands, which is responsible for the SERS enhancement. The as-fabricated SERS active platform shows a linear response in the physiological range (10 nM to 100 μM) and an extremely low limit of detection (LOD) of 1.76 nM with a correlation coefficient of 0.98 and enhancement factor ~2 × 10<sup>7</sup>. The findings suggest that our fabricated SERS platform could be potentially used for the rapid detection of bio-chemical traces with high sensitivity. |
| first_indexed | 2024-03-09T18:08:25Z |
| format | Article |
| id | doaj.art-ee12b4e719384d19adc042d2b1eb669b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:08:25Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ee12b4e719384d19adc042d2b1eb669b2023-11-24T09:21:42ZengMDPI AGMolecules1420-30492022-11-012722782110.3390/molecules27227821Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer BiomarkersAnamika Pandey0Subhankar Sarkar1Sumit Kumar Pandey2Anchal Srivastava3Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaSchool of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaDepartment of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaThe in vitro diagnostics of cancer are not represented well yet, but the need for early-stage detection is undeniable. In recent decades, surface-enhanced Raman spectroscopy (SERS) has emerged as an efficient, adaptable, and unique technique for the detection of cancer molecules in their early stages. Herein, we demonstrate an opto-plasmonic hybrid structure for sensitive detection of the prostate cancer biomarker sarcosine using silica nanospheres coated silver nano-islands as a facile and efficient SERS active substrate. The SERS active platform has been developed via thin (5–15 nm) deposition of silver islands using a simple and cost-effective Radio Frequency (RF) sputtering technique followed by the synthesis and decoration of silica nanospheres (~500 nm) synthesized via Stober’s method. It is anticipated that the coupling of Whispering Gallery Modes and photonic nano-jets in SiO<sub>2</sub> nanospheres induce Localized Surface Plasmon Resonance (LSPR) in Ag nano-islands, which is responsible for the SERS enhancement. The as-fabricated SERS active platform shows a linear response in the physiological range (10 nM to 100 μM) and an extremely low limit of detection (LOD) of 1.76 nM with a correlation coefficient of 0.98 and enhancement factor ~2 × 10<sup>7</sup>. The findings suggest that our fabricated SERS platform could be potentially used for the rapid detection of bio-chemical traces with high sensitivity.https://www.mdpi.com/1420-3049/27/22/7821silica nanosphereSERSsarcosinesilver islandsWGM resonancephotonic nano-jets |
| spellingShingle | Anamika Pandey Subhankar Sarkar Sumit Kumar Pandey Anchal Srivastava Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers Molecules silica nanosphere SERS sarcosine silver islands WGM resonance photonic nano-jets |
| title | Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers |
| title_full | Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers |
| title_fullStr | Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers |
| title_full_unstemmed | Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers |
| title_short | Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers |
| title_sort | silica nanospheres coated silver islands as an effective opto plasmonic sers active platform for rapid and sensitive detection of prostate cancer biomarkers |
| topic | silica nanosphere SERS sarcosine silver islands WGM resonance photonic nano-jets |
| url | https://www.mdpi.com/1420-3049/27/22/7821 |
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