Lipids from Lipomyces starkeyi
The oleaginous yeast Lipomyces starkeyi is interesting for industrial-scale production of biodiesel. Here it is reported that this yeast is able to digest unsaccharified, soluble potato starch. In these experiments, the cultivation time of Lipomyces starkeyi was reduced to half of the previously rep...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2010-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/87218 |
| _version_ | 1827614912774406144 |
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| author | Robert Wild Satish Patil Milan Popović Mark Zappi Stephen Dufreche Rakesh Bajpai |
| author_facet | Robert Wild Satish Patil Milan Popović Mark Zappi Stephen Dufreche Rakesh Bajpai |
| author_sort | Robert Wild |
| collection | DOAJ |
| description | The oleaginous yeast Lipomyces starkeyi is interesting for industrial-scale production of biodiesel. Here it is reported that this yeast is able to digest unsaccharified, soluble potato starch. In these experiments, the cultivation time of Lipomyces starkeyi was reduced to half of the previously reported values. The effect of C:N molar ratio was studied on growth and lipid content of L. starkeyi in media containing glucose or potato starch as C-source. At a C:N molar ratio of 61.2 in glucose medium, the lipid content was measured at 30 % of the dry matter. It was established that the cellular lipid content increased with increasing C:N molar ratio, but the cell yield decreased. In a pH-controlled 1-litre agitated and aerated bioreactor, batch cultivation on glucose resulted in 23 % lipids in cells on dry mass basis (dm), cell yield of 0.25 g/g glucose, and lipid yield of 0.06 g/g glucose. Under the same operating conditions, fed-batch cultivation with discrete glucose additions resulted in 27 % lipids, cell yield of 0.28 g/g glucose, and lipid yield of 0.08 g/g glucose. On soluble but unsaccharified sweet potato starch, cell yield was 0.41 g/g glucose equivalent, lipid yield 0.16 g/g glucose equivalent, and lipid content in cells 40 %. The major fatty acids in the cells were C16:0 and C18:1 (accounting for 85–90 % by mass of the total lipids) with the rest being C16:1 and C18:0. All of these are valuable lipids for biodiesel production. Based on these results, wastes and wastewater from food industry and sewage sludge could serve as sources for the production of biodiesel. |
| first_indexed | 2024-03-09T09:02:41Z |
| format | Article |
| id | doaj.art-ee155dbae9514e5b9fafeaa0be775039 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:02:41Z |
| publishDate | 2010-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-ee155dbae9514e5b9fafeaa0be7750392023-12-02T11:22:19ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062010-01-01483329335Lipids from Lipomyces starkeyiRobert Wild0Satish Patil1Milan Popović2Mark Zappi3Stephen Dufreche4Rakesh Bajpai5Beuth University of Applied Sciences, Department for Life Sciences and Technology Berlin, See Str. 64, DE-13347 Berlin, GermanyChemical Engineering Department, University of Louisiana at Lafayette, PO Box 44130, Lafayette, LA 70508, USABeuth University of Applied Sciences, Department for Life Sciences and Technology Berlin, See Str. 64, DE-13347 Berlin, GermanyChemical Engineering Department, University of Louisiana at Lafayette, PO Box 44130, Lafayette, LA 70508, USAChemical Engineering Department, University of Louisiana at Lafayette, PO Box 44130, Lafayette, LA 70508, USAChemical Engineering Department, University of Louisiana at Lafayette, PO Box 44130, Lafayette, LA 70508, USAThe oleaginous yeast Lipomyces starkeyi is interesting for industrial-scale production of biodiesel. Here it is reported that this yeast is able to digest unsaccharified, soluble potato starch. In these experiments, the cultivation time of Lipomyces starkeyi was reduced to half of the previously reported values. The effect of C:N molar ratio was studied on growth and lipid content of L. starkeyi in media containing glucose or potato starch as C-source. At a C:N molar ratio of 61.2 in glucose medium, the lipid content was measured at 30 % of the dry matter. It was established that the cellular lipid content increased with increasing C:N molar ratio, but the cell yield decreased. In a pH-controlled 1-litre agitated and aerated bioreactor, batch cultivation on glucose resulted in 23 % lipids in cells on dry mass basis (dm), cell yield of 0.25 g/g glucose, and lipid yield of 0.06 g/g glucose. Under the same operating conditions, fed-batch cultivation with discrete glucose additions resulted in 27 % lipids, cell yield of 0.28 g/g glucose, and lipid yield of 0.08 g/g glucose. On soluble but unsaccharified sweet potato starch, cell yield was 0.41 g/g glucose equivalent, lipid yield 0.16 g/g glucose equivalent, and lipid content in cells 40 %. The major fatty acids in the cells were C16:0 and C18:1 (accounting for 85–90 % by mass of the total lipids) with the rest being C16:1 and C18:0. All of these are valuable lipids for biodiesel production. Based on these results, wastes and wastewater from food industry and sewage sludge could serve as sources for the production of biodiesel.http://hrcak.srce.hr/file/87218Lipomyces starkeyibiodiesellipidsyeaststarchfatty acid methyl esters |
| spellingShingle | Robert Wild Satish Patil Milan Popović Mark Zappi Stephen Dufreche Rakesh Bajpai Lipids from Lipomyces starkeyi Food Technology and Biotechnology Lipomyces starkeyi biodiesel lipids yeast starch fatty acid methyl esters |
| title | Lipids from Lipomyces starkeyi |
| title_full | Lipids from Lipomyces starkeyi |
| title_fullStr | Lipids from Lipomyces starkeyi |
| title_full_unstemmed | Lipids from Lipomyces starkeyi |
| title_short | Lipids from Lipomyces starkeyi |
| title_sort | lipids from lipomyces starkeyi |
| topic | Lipomyces starkeyi biodiesel lipids yeast starch fatty acid methyl esters |
| url | http://hrcak.srce.hr/file/87218 |
| work_keys_str_mv | AT robertwild lipidsfromlipomycesstarkeyi AT satishpatil lipidsfromlipomycesstarkeyi AT milanpopovic lipidsfromlipomycesstarkeyi AT markzappi lipidsfromlipomycesstarkeyi AT stephendufreche lipidsfromlipomycesstarkeyi AT rakeshbajpai lipidsfromlipomycesstarkeyi |