Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro
A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci wa...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2012-11-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201211-0005_probiotic-properties-of-enterococcus-faecium-ce5-1-producing-a-bacteriocin-like-substance-and-its-antagonistic.php |
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| author | K. Saelim N. Sohsomboon S. Kaewsuwan S. Maneerat |
| author_facet | K. Saelim N. Sohsomboon S. Kaewsuwan S. Maneerat |
| author_sort | K. Saelim |
| collection | DOAJ |
| description | A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2-3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci. |
| first_indexed | 2024-04-10T08:26:42Z |
| format | Article |
| id | doaj.art-ee1a57d311ed4183b01c60a6b8c79b5f |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:42Z |
| publishDate | 2012-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-ee1a57d311ed4183b01c60a6b8c79b5f2023-02-23T03:33:00ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092012-11-01571152953910.17221/6386-CJAScjs-201211-0005Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitroK. Saelim0N. Sohsomboon1S. Kaewsuwan2S. Maneerat3Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, ThailandDepartment of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, ThailandDepartment of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, ThailandA bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2-3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci.https://cjas.agriculturejournals.cz/artkey/cjs-201211-0005_probiotic-properties-of-enterococcus-faecium-ce5-1-producing-a-bacteriocin-like-substance-and-its-antagonistic.phpchickengastrointestinal tractbile saltacid toleranceenterococcus |
| spellingShingle | K. Saelim N. Sohsomboon S. Kaewsuwan S. Maneerat Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro Czech Journal of Animal Science chicken gastrointestinal tract bile salt acid tolerance enterococcus |
| title | Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro |
| title_full | Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro |
| title_fullStr | Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro |
| title_full_unstemmed | Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro |
| title_short | Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro |
| title_sort | probiotic properties of enterococcus faecium ce5 1 producing a bacteriocin like substance and its antagonistic effect against antibiotic resistant enterococci in vitro |
| topic | chicken gastrointestinal tract bile salt acid tolerance enterococcus |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201211-0005_probiotic-properties-of-enterococcus-faecium-ce5-1-producing-a-bacteriocin-like-substance-and-its-antagonistic.php |
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