Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1
Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA inte...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-07-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-43-7-1104.pdf |
| _version_ | 1797361468389195776 |
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| author | ZENG Yankun, HONG Xiaoying, CHEN Qinglin |
| author_facet | ZENG Yankun, HONG Xiaoying, CHEN Qinglin |
| author_sort | ZENG Yankun, HONG Xiaoying, CHEN Qinglin |
| collection | DOAJ |
| description | Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9. |
| first_indexed | 2024-03-08T15:54:08Z |
| format | Article |
| id | doaj.art-ee1ae8bac24341d9b226925ede9dd8c7 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:54:08Z |
| publishDate | 2023-07-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-ee1ae8bac24341d9b226925ede9dd8c72024-01-09T03:11:02ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-07-014371104110910.16352/j.issn.1001-6325.2023.07.1104Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1ZENG Yankun, HONG Xiaoying, CHEN Qinglin01. Department of Health,Quanzhou Medical College,Quanzhou 362000;;2. School of Medical Technology and Engineering,Fujian Medical University,Fuzhou 350004;;3. Department of Clinical Laboratory, the Affiliated Quanzhou First Hospital of Fujian Medical University,Quanzhou 362000,ChinaObjective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-43-7-1104.pdfbecn1|leukemia|proliferation|invasion|migration |
| spellingShingle | ZENG Yankun, HONG Xiaoying, CHEN Qinglin Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 Jichu yixue yu linchuang becn1|leukemia|proliferation|invasion|migration |
| title | Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 |
| title_full | Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 |
| title_fullStr | Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 |
| title_full_unstemmed | Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 |
| title_short | Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 |
| title_sort | down regulation of becn1 inhibits the proliferation invasion and migration of human leukemia cell line thp 1 |
| topic | becn1|leukemia|proliferation|invasion|migration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-43-7-1104.pdf |
| work_keys_str_mv | AT zengyankunhongxiaoyingchenqinglin downregulationofbecn1inhibitstheproliferationinvasionandmigrationofhumanleukemiacelllinethp1 |