Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection
<i>Haemaphysalis longicornis</i> is a tick and a vector of various pathogens, including the human pathogenetic <i>Babesia microti</i>. The objective of this study was to identify female <i>H. longicornis</i> genes differentially expressed in response to infection...
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MDPI AG
2020-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/5/378 |
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| author | Weiqing Zheng Rika Umemiya-Shirafuji Shengen Chen Kiyoshi Okado Paul Franck Adjou Moumouni Hiroshi Suzuki Shu Yang Mingming Liu Xuenan Xuan |
| author_facet | Weiqing Zheng Rika Umemiya-Shirafuji Shengen Chen Kiyoshi Okado Paul Franck Adjou Moumouni Hiroshi Suzuki Shu Yang Mingming Liu Xuenan Xuan |
| author_sort | Weiqing Zheng |
| collection | DOAJ |
| description | <i>Haemaphysalis longicornis</i> is a tick and a vector of various pathogens, including the human pathogenetic <i>Babesia microti</i>. The objective of this study was to identify female <i>H. longicornis</i> genes differentially expressed in response to infection with <i>B. microti </i>Gray strain by using a suppression subtractive hybridization (SSH) procedure. A total of 302 randomly selected clones were sequenced and analyzed in the forward subtracted SSH cDNA library related to <i>Babesia</i> infection, and 110 clones in the reverse cDNA library. Gene ontology assignments and sequence analyses of tick sequences in the forward cDNA library showed that 14 genes were related to response to stimulus or/and immune system process, and 7 genes had the higher number of standardized sequences per kilobase (SPK). Subsequent real-time PCR detection showed that eight genes including those encoding for Obg-like ATPase 1 (ola1), Calreticulin (crt), vitellogenin 1 (Vg1) and Vg2 were up-regulated in fed ticks. Compared to uninfected ticks, infected ticks had six up-regulated genes, including <i>ola1</i>, <i>crt</i> and <i>Vg2</i>. Functional analysis of up-regulated genes in fed or<i> Babesia</i>-infected ticks by RNA interference showed that knockdown of<i> crt</i> and <i>Vg2 </i>in infected ticks and knockdown of<i> ola1</i> in uninfected ticks accelerated engorgement. In contrast, <i>Vg1</i> knockdown in infected ticks had delayed engorgement. Knockdown of<i> crt</i> and <i>Vg1 </i>in infected ticks decreased engorged female weight. <i>Vg2</i> knockdown reduced <i>B. microti</i> infection levels by 51% when compared with controls. The results reported here increase our understanding of roles of <i>H. longicornis</i> genes in blood feeding and <i>B. microti</i> infection. |
| first_indexed | 2024-03-10T19:50:06Z |
| format | Article |
| id | doaj.art-ee1c21f1acdd4eec9426677376a5c983 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:50:06Z |
| publishDate | 2020-05-01 |
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| series | Pathogens |
| spelling | doaj.art-ee1c21f1acdd4eec9426677376a5c9832023-11-20T00:29:03ZengMDPI AGPathogens2076-08172020-05-019537810.3390/pathogens9050378Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> InfectionWeiqing Zheng0Rika Umemiya-Shirafuji1Shengen Chen2Kiyoshi Okado3Paul Franck Adjou Moumouni4Hiroshi Suzuki5Shu Yang6Mingming Liu7Xuenan Xuan8The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases, Nanchang Center for Disease Control and Prevention, Honggutan New District, Nanchang, Jiangxi 330038, ChinaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases, Nanchang Center for Disease Control and Prevention, Honggutan New District, Nanchang, Jiangxi 330038, ChinaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases, Nanchang Center for Disease Control and Prevention, Honggutan New District, Nanchang, Jiangxi 330038, ChinaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan<i>Haemaphysalis longicornis</i> is a tick and a vector of various pathogens, including the human pathogenetic <i>Babesia microti</i>. The objective of this study was to identify female <i>H. longicornis</i> genes differentially expressed in response to infection with <i>B. microti </i>Gray strain by using a suppression subtractive hybridization (SSH) procedure. A total of 302 randomly selected clones were sequenced and analyzed in the forward subtracted SSH cDNA library related to <i>Babesia</i> infection, and 110 clones in the reverse cDNA library. Gene ontology assignments and sequence analyses of tick sequences in the forward cDNA library showed that 14 genes were related to response to stimulus or/and immune system process, and 7 genes had the higher number of standardized sequences per kilobase (SPK). Subsequent real-time PCR detection showed that eight genes including those encoding for Obg-like ATPase 1 (ola1), Calreticulin (crt), vitellogenin 1 (Vg1) and Vg2 were up-regulated in fed ticks. Compared to uninfected ticks, infected ticks had six up-regulated genes, including <i>ola1</i>, <i>crt</i> and <i>Vg2</i>. Functional analysis of up-regulated genes in fed or<i> Babesia</i>-infected ticks by RNA interference showed that knockdown of<i> crt</i> and <i>Vg2 </i>in infected ticks and knockdown of<i> ola1</i> in uninfected ticks accelerated engorgement. In contrast, <i>Vg1</i> knockdown in infected ticks had delayed engorgement. Knockdown of<i> crt</i> and <i>Vg1 </i>in infected ticks decreased engorged female weight. <i>Vg2</i> knockdown reduced <i>B. microti</i> infection levels by 51% when compared with controls. The results reported here increase our understanding of roles of <i>H. longicornis</i> genes in blood feeding and <i>B. microti</i> infection.https://www.mdpi.com/2076-0817/9/5/378<i>Haemaphysalis longicornis</i><i>Babesia microti</i>vector-pathogen interactionsRNA interferencevaccine |
| spellingShingle | Weiqing Zheng Rika Umemiya-Shirafuji Shengen Chen Kiyoshi Okado Paul Franck Adjou Moumouni Hiroshi Suzuki Shu Yang Mingming Liu Xuenan Xuan Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection Pathogens <i>Haemaphysalis longicornis</i> <i>Babesia microti</i> vector-pathogen interactions RNA interference vaccine |
| title | Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection |
| title_full | Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection |
| title_fullStr | Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection |
| title_full_unstemmed | Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection |
| title_short | Identification of <i>Haemaphysalis longicornis</i> Genes Differentially Expressed in Response to <i>Babesia microti</i> Infection |
| title_sort | identification of i haemaphysalis longicornis i genes differentially expressed in response to i babesia microti i infection |
| topic | <i>Haemaphysalis longicornis</i> <i>Babesia microti</i> vector-pathogen interactions RNA interference vaccine |
| url | https://www.mdpi.com/2076-0817/9/5/378 |
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