Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents
Theranostic radioisotope pairs such as Gallium-68 (<sup>68</sup>Ga) for Positron Emission Tomography (PET) and Lutetium-177 (<sup>177</sup>Lu) for radioisotopic therapy, in conjunction with nanoparticles (NPs), are an emerging field in the treatment of cancer. The present wor...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/14/2490 |
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| author | Evangelia-Alexandra Salvanou Argiris Kolokithas-Ntoukas Christos Liolios Stavros Xanthopoulos Maria Paravatou-Petsotas Charalampos Tsoukalas Konstantinos Avgoustakis Penelope Bouziotis |
| author_facet | Evangelia-Alexandra Salvanou Argiris Kolokithas-Ntoukas Christos Liolios Stavros Xanthopoulos Maria Paravatou-Petsotas Charalampos Tsoukalas Konstantinos Avgoustakis Penelope Bouziotis |
| author_sort | Evangelia-Alexandra Salvanou |
| collection | DOAJ |
| description | Theranostic radioisotope pairs such as Gallium-68 (<sup>68</sup>Ga) for Positron Emission Tomography (PET) and Lutetium-177 (<sup>177</sup>Lu) for radioisotopic therapy, in conjunction with nanoparticles (NPs), are an emerging field in the treatment of cancer. The present work aims to demonstrate the ability of condensed colloidal nanocrystal clusters (co-CNCs) comprised of iron oxide nanoparticles, coated with alginic acid (MA) and stabilized by a layer of polyethylene glycol (MAPEG) to be directly radiolabeled with <sup>68</sup>Ga and its therapeutic analog <sup>177</sup>Lu. <sup>68</sup>Ga/<sup>177</sup>Lu- MA and MAPEG were investigated for their in vitro stability. The biocompatibility of the non-radiolabeled nanoparticles, as well as the cytotoxicity of MA, MAPEG, and [<sup>177</sup>Lu]Lu-MAPEG were assessed on 4T1 cells. Finally, the ex vivo biodistribution of the <sup>68</sup>Ga-labeled NPs as well as [<sup>177</sup>Lu]Lu-MAPEG was investigated in normal mice. Radiolabeling with both radioisotopes took place via a simple and direct labelling method without further purification. Hemocompatibility was verified for both NPs, while MTT studies demonstrated the non-cytotoxic profile of the nanocarriers and the dose-dependent toxicity for [<sup>177</sup>Lu]Lu-MAPEG. The radiolabeled nanoparticles mainly accumulated in RES organs. Based on our preliminary results, we conclude that MAPEG could be further investigated as a theranostic agent for PET diagnosis and therapy of cancer. |
| first_indexed | 2024-03-09T06:06:49Z |
| format | Article |
| id | doaj.art-ee1d71dbf05a4325be6313ef5fe69d1d |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T06:06:49Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ee1d71dbf05a4325be6313ef5fe69d1d2023-12-03T12:03:43ZengMDPI AGNanomaterials2079-49912022-07-011214249010.3390/nano12142490Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic AgentsEvangelia-Alexandra Salvanou0Argiris Kolokithas-Ntoukas1Christos Liolios2Stavros Xanthopoulos3Maria Paravatou-Petsotas4Charalampos Tsoukalas5Konstantinos Avgoustakis6Penelope Bouziotis7Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceDepartment of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceDepartment of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, GreeceInstitute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Center for Scientific Research “Demokritos”, 15341 Athens, GreeceTheranostic radioisotope pairs such as Gallium-68 (<sup>68</sup>Ga) for Positron Emission Tomography (PET) and Lutetium-177 (<sup>177</sup>Lu) for radioisotopic therapy, in conjunction with nanoparticles (NPs), are an emerging field in the treatment of cancer. The present work aims to demonstrate the ability of condensed colloidal nanocrystal clusters (co-CNCs) comprised of iron oxide nanoparticles, coated with alginic acid (MA) and stabilized by a layer of polyethylene glycol (MAPEG) to be directly radiolabeled with <sup>68</sup>Ga and its therapeutic analog <sup>177</sup>Lu. <sup>68</sup>Ga/<sup>177</sup>Lu- MA and MAPEG were investigated for their in vitro stability. The biocompatibility of the non-radiolabeled nanoparticles, as well as the cytotoxicity of MA, MAPEG, and [<sup>177</sup>Lu]Lu-MAPEG were assessed on 4T1 cells. Finally, the ex vivo biodistribution of the <sup>68</sup>Ga-labeled NPs as well as [<sup>177</sup>Lu]Lu-MAPEG was investigated in normal mice. Radiolabeling with both radioisotopes took place via a simple and direct labelling method without further purification. Hemocompatibility was verified for both NPs, while MTT studies demonstrated the non-cytotoxic profile of the nanocarriers and the dose-dependent toxicity for [<sup>177</sup>Lu]Lu-MAPEG. The radiolabeled nanoparticles mainly accumulated in RES organs. Based on our preliminary results, we conclude that MAPEG could be further investigated as a theranostic agent for PET diagnosis and therapy of cancer.https://www.mdpi.com/2079-4991/12/14/2490Gallium-68Lutetium-177iron oxide nanoparticlescondensed clustersMTTradiolabeling |
| spellingShingle | Evangelia-Alexandra Salvanou Argiris Kolokithas-Ntoukas Christos Liolios Stavros Xanthopoulos Maria Paravatou-Petsotas Charalampos Tsoukalas Konstantinos Avgoustakis Penelope Bouziotis Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents Nanomaterials Gallium-68 Lutetium-177 iron oxide nanoparticles condensed clusters MTT radiolabeling |
| title | Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents |
| title_full | Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents |
| title_fullStr | Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents |
| title_full_unstemmed | Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents |
| title_short | Preliminary Evaluation of Iron Oxide Nanoparticles Radiolabeled with <sup>68</sup>Ga and <sup>177</sup>Lu as Potential Theranostic Agents |
| title_sort | preliminary evaluation of iron oxide nanoparticles radiolabeled with sup 68 sup ga and sup 177 sup lu as potential theranostic agents |
| topic | Gallium-68 Lutetium-177 iron oxide nanoparticles condensed clusters MTT radiolabeling |
| url | https://www.mdpi.com/2079-4991/12/14/2490 |
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