Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-rea...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1406460 |
| _version_ | 1818394688549289984 |
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| author | Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang |
| author_facet | Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang |
| author_sort | Daniel Mukunzi |
| collection | DOAJ |
| description | An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food. |
| first_indexed | 2024-12-14T06:05:11Z |
| format | Article |
| id | doaj.art-ee1d7fcf872c4e8b85551d0964637079 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-14T06:05:11Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-ee1d7fcf872c4e8b85551d09646370792022-12-21T23:14:20ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129148449710.1080/09540105.2017.14064601406460Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samplesDaniel Mukunzi0Steven Suryoprabowo1Shanshan Song2Liqiang Liu3Hua Kuang4State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityAn anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food.http://dx.doi.org/10.1080/09540105.2017.1406460pefloxacinmonoclonal antibodyindirect competitive enzyme-linked immunosorbent assaylateral flowstrip |
| spellingShingle | Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples Food and Agricultural Immunology pefloxacin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay lateral flow strip |
| title | Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples |
| title_full | Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples |
| title_fullStr | Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples |
| title_full_unstemmed | Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples |
| title_short | Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples |
| title_sort | development of an indirect enzyme linked immunosorbent assay and lateral flow test strips for pefloxacin and its analogues in chicken muscle samples |
| topic | pefloxacin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay lateral flow strip |
| url | http://dx.doi.org/10.1080/09540105.2017.1406460 |
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