An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis
Background:. Distraction osteogenesis (DO) is used for skeletal defects; however, up to 50% of cases exhibit complications. Previous mouse models of long bone DO have been anecdotally hampered by postoperative complications, expense, and availability. To improve clinical techniques, cost-effective,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2023-02-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000004674 |
| _version_ | 1828003875967205376 |
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| author | Ruth Tevlin, MB, BAO, BCh, MRCSI, MD Harsh N. Shah, MPH, MS Ankit Salhotra, BS Sarah E. Di Iorio, SB Michelle Griffin, MBChB, MRCS, PhD Michael Januszyk, MD, PhD Derrick C. Wan, MD Michael T. Longaker, MD, MBA, FACS |
| author_facet | Ruth Tevlin, MB, BAO, BCh, MRCSI, MD Harsh N. Shah, MPH, MS Ankit Salhotra, BS Sarah E. Di Iorio, SB Michelle Griffin, MBChB, MRCS, PhD Michael Januszyk, MD, PhD Derrick C. Wan, MD Michael T. Longaker, MD, MBA, FACS |
| author_sort | Ruth Tevlin, MB, BAO, BCh, MRCSI, MD |
| collection | DOAJ |
| description | Background:. Distraction osteogenesis (DO) is used for skeletal defects; however, up to 50% of cases exhibit complications. Previous mouse models of long bone DO have been anecdotally hampered by postoperative complications, expense, and availability. To improve clinical techniques, cost-effective, reliable animal models are needed. Our focus was to develop a new mouse tibial distractor, hypothesized to result in successful, complication-free DO.
Methods:. A lightweight tibial distractor was developed using CAD and 3D printing. The device was fixed to the tibia of C57Bl/6J mice prior to osteotomy. Postoperatively, mice underwent 5 days latency, 10 days distraction (0.15 mm every 12 hours), and 28 days consolidation. Bone regeneration was examined on postoperative day 43 using micro-computed tomography (μCT) and Movat’s modified pentachrome staining on histology (mineralized volume fraction and pixels, respectively). Costs were recorded. We compared cohorts of 11 mice undergoing sham, DO, or acute lengthening (distractor acutely lengthened 3.0 mm).
Results:. The histological bone regenerate was significantly increased in DO (1,879,257 ± 155,415 pixels) compared to acute lengthening (32847 ± 1589 pixels) (P < 0.0001). The mineralized volume fraction (bone/total tissue volume) of the regenerate was significantly increased in DO (0.9 ± 0.1) compared to acute lengthening (0.7 ± 0.1) (P < 0.001). There was no significant difference in bone regenerate between DO and sham. The distractor was relatively low cost ($11), with no complications.
Conclusions:. Histology and µCT analysis confirmed that the proposed tibial DO model resulted in successful bone formation. Our model is cost-effective and reproducible, enabling implementation in genetically dissectible transgenic mice. |
| first_indexed | 2024-04-10T07:05:38Z |
| format | Article |
| id | doaj.art-ee2fbf179619420799d4e6038f8fbef9 |
| institution | Directory Open Access Journal |
| issn | 2169-7574 |
| language | English |
| last_indexed | 2024-04-10T07:05:38Z |
| publishDate | 2023-02-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj.art-ee2fbf179619420799d4e6038f8fbef92023-02-27T09:02:01ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742023-02-01112e467410.1097/GOX.0000000000004674202302000-00010An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction OsteogenesisRuth Tevlin, MB, BAO, BCh, MRCSI, MD0Harsh N. Shah, MPH, MS1Ankit Salhotra, BS2Sarah E. Di Iorio, SB3Michelle Griffin, MBChB, MRCS, PhD4Michael Januszyk, MD, PhD5Derrick C. Wan, MD6Michael T. Longaker, MD, MBA, FACS7From the * Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.† Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, Calif.† Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, Calif.† Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, Calif.† Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, Calif.† Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, Calif.From the * Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.From the * Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, Calif.Background:. Distraction osteogenesis (DO) is used for skeletal defects; however, up to 50% of cases exhibit complications. Previous mouse models of long bone DO have been anecdotally hampered by postoperative complications, expense, and availability. To improve clinical techniques, cost-effective, reliable animal models are needed. Our focus was to develop a new mouse tibial distractor, hypothesized to result in successful, complication-free DO. Methods:. A lightweight tibial distractor was developed using CAD and 3D printing. The device was fixed to the tibia of C57Bl/6J mice prior to osteotomy. Postoperatively, mice underwent 5 days latency, 10 days distraction (0.15 mm every 12 hours), and 28 days consolidation. Bone regeneration was examined on postoperative day 43 using micro-computed tomography (μCT) and Movat’s modified pentachrome staining on histology (mineralized volume fraction and pixels, respectively). Costs were recorded. We compared cohorts of 11 mice undergoing sham, DO, or acute lengthening (distractor acutely lengthened 3.0 mm). Results:. The histological bone regenerate was significantly increased in DO (1,879,257 ± 155,415 pixels) compared to acute lengthening (32847 ± 1589 pixels) (P < 0.0001). The mineralized volume fraction (bone/total tissue volume) of the regenerate was significantly increased in DO (0.9 ± 0.1) compared to acute lengthening (0.7 ± 0.1) (P < 0.001). There was no significant difference in bone regenerate between DO and sham. The distractor was relatively low cost ($11), with no complications. Conclusions:. Histology and µCT analysis confirmed that the proposed tibial DO model resulted in successful bone formation. Our model is cost-effective and reproducible, enabling implementation in genetically dissectible transgenic mice.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000004674 |
| spellingShingle | Ruth Tevlin, MB, BAO, BCh, MRCSI, MD Harsh N. Shah, MPH, MS Ankit Salhotra, BS Sarah E. Di Iorio, SB Michelle Griffin, MBChB, MRCS, PhD Michael Januszyk, MD, PhD Derrick C. Wan, MD Michael T. Longaker, MD, MBA, FACS An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis Plastic and Reconstructive Surgery, Global Open |
| title | An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis |
| title_full | An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis |
| title_fullStr | An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis |
| title_full_unstemmed | An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis |
| title_short | An Inexpensive 3D Printed Mouse Model of Successful, Complication-free Long Bone Distraction Osteogenesis |
| title_sort | inexpensive 3d printed mouse model of successful complication free long bone distraction osteogenesis |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000004674 |
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