Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i>
The plants in the <i>Sideritis</i> genus are postulated to exhibit several important medicinal properties due to their unique chemical composition. To isolate the targeted phytochemical compounds, the selection of a suitable extraction method is of primary importance. In this work, a com...
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/10/4207 |
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| author | Marika Mróz Edyta Malinowska-Pańczyk Agnieszka Bartoszek Barbara Kusznierewicz |
| author_facet | Marika Mróz Edyta Malinowska-Pańczyk Agnieszka Bartoszek Barbara Kusznierewicz |
| author_sort | Marika Mróz |
| collection | DOAJ |
| description | The plants in the <i>Sideritis</i> genus are postulated to exhibit several important medicinal properties due to their unique chemical composition. To isolate the targeted phytochemical compounds, the selection of a suitable extraction method is of primary importance. In this work, a comparative study on the phytochemical profiles of various <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> extracts has been carried out. An untargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was applied to investigate the metabolic differences between extracts obtained by conventional extraction and extractions assisted by microwaves, ultrasounds and high pressure. Additionally, the influence of extraction solvents on HPLC antioxidant profiles obtained following the derivatization of analytes with ABTS reagent was evaluated. A total of 102 metabolites have been putatively identified. The major secondary metabolites groups were classified as flavonoids, terpenoids, phenylethanoid glycosides and phenolic acids. The main antioxidants in the extracts were isoscutellarein and hypolaetin derivatives as well as verbascoside and chlorogenic acid. The results showed that 70% ethanol was the most effective extractant for different classes of phytochemicals including antioxidants. In addition, extraction supported with microwaves, ultrasounds or high pressure improved the overall recovery of metabolites by about 3 times compared to the conventional extraction method. |
| first_indexed | 2024-03-11T03:26:55Z |
| format | Article |
| id | doaj.art-ee300e68cdfe4db7825f3f8bfe8cc64a |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:26:55Z |
| publishDate | 2023-05-01 |
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| series | Molecules |
| spelling | doaj.art-ee300e68cdfe4db7825f3f8bfe8cc64a2023-11-18T02:40:53ZengMDPI AGMolecules1420-30492023-05-012810420710.3390/molecules28104207Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i>Marika Mróz0Edyta Malinowska-Pańczyk1Agnieszka Bartoszek2Barbara Kusznierewicz3Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk, PolandDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk, PolandDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk, PolandDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk, PolandThe plants in the <i>Sideritis</i> genus are postulated to exhibit several important medicinal properties due to their unique chemical composition. To isolate the targeted phytochemical compounds, the selection of a suitable extraction method is of primary importance. In this work, a comparative study on the phytochemical profiles of various <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> extracts has been carried out. An untargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was applied to investigate the metabolic differences between extracts obtained by conventional extraction and extractions assisted by microwaves, ultrasounds and high pressure. Additionally, the influence of extraction solvents on HPLC antioxidant profiles obtained following the derivatization of analytes with ABTS reagent was evaluated. A total of 102 metabolites have been putatively identified. The major secondary metabolites groups were classified as flavonoids, terpenoids, phenylethanoid glycosides and phenolic acids. The main antioxidants in the extracts were isoscutellarein and hypolaetin derivatives as well as verbascoside and chlorogenic acid. The results showed that 70% ethanol was the most effective extractant for different classes of phytochemicals including antioxidants. In addition, extraction supported with microwaves, ultrasounds or high pressure improved the overall recovery of metabolites by about 3 times compared to the conventional extraction method.https://www.mdpi.com/1420-3049/28/10/4207<i>Sideritis raeseri</i><i>Sideritis scardica</i>extractionUPLC-HRMSantioxidantsMAE |
| spellingShingle | Marika Mróz Edyta Malinowska-Pańczyk Agnieszka Bartoszek Barbara Kusznierewicz Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> Molecules <i>Sideritis raeseri</i> <i>Sideritis scardica</i> extraction UPLC-HRMS antioxidants MAE |
| title | Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> |
| title_full | Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> |
| title_fullStr | Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> |
| title_full_unstemmed | Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> |
| title_short | Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from <i>Sideritis raeseri</i> and <i>Sideritis scardica</i> |
| title_sort | comparative study on assisted solvent extraction techniques for the extraction of biologically active compounds from i sideritis raeseri i and i sideritis scardica i |
| topic | <i>Sideritis raeseri</i> <i>Sideritis scardica</i> extraction UPLC-HRMS antioxidants MAE |
| url | https://www.mdpi.com/1420-3049/28/10/4207 |
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