<i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex
The pathogen causing Fusarium wilt in banana is reported to be <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (FOC). In 2019, wilt symptoms in banana plants (cultivar: Cavendish) in the Philippines were detected, i.e., the yellowing of the leaves and discoloration of the pse...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/4/443 |
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| author | Shunsuke Nozawa Yosuke Seto Yoshiki Takata Lalaine Albano Narreto Reynaldo R. Valle Keiju Okui Shigeya Taida Dionisio G. Alvindia Renato G. Reyes Kyoko Watanabe |
| author_facet | Shunsuke Nozawa Yosuke Seto Yoshiki Takata Lalaine Albano Narreto Reynaldo R. Valle Keiju Okui Shigeya Taida Dionisio G. Alvindia Renato G. Reyes Kyoko Watanabe |
| author_sort | Shunsuke Nozawa |
| collection | DOAJ |
| description | The pathogen causing Fusarium wilt in banana is reported to be <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (FOC). In 2019, wilt symptoms in banana plants (cultivar: Cavendish) in the Philippines were detected, i.e., the yellowing of the leaves and discoloration of the pseudostem and vascular tissue. The fungus isolated from the vascular tissue was found to be pathogenic to Cavendish bananas and was identified as a new species, <i>F. mindanaoense</i>, belonging to the <i>F. fujikuroi</i> species complex (FFSC); species classification was assessed using molecular phylogenetic analyses based on the <i>tef1</i>, <i>tub2</i>, <i>cmdA</i>, <i>rpb1</i>, and <i>rpb2</i> genes and morphological analyses. A reciprocal blast search using genomic data revealed that this fungus exclusively included the <i>Secreted in Xylem 6</i> (<i>SIX6</i>) gene among the <i>SIX</i> homologs related to pathogenicity; it exhibited a highly conserved amino acid sequence compared with that of species in the FFSC, but not with that of FOC. This was the first report of Fusarium wilt in Cavendish bananas caused by a species of the genus <i>Fusarium</i> other than those in the <i>F. oxysporum</i> species complex. |
| first_indexed | 2024-03-11T04:52:31Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T04:52:31Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-ee31fd80ebeb4e74863631999669f8082023-11-17T19:58:13ZengMDPI AGJournal of Fungi2309-608X2023-04-019444310.3390/jof9040443<i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species ComplexShunsuke Nozawa0Yosuke Seto1Yoshiki Takata2Lalaine Albano Narreto3Reynaldo R. Valle4Keiju Okui5Shigeya Taida6Dionisio G. Alvindia7Renato G. Reyes8Kyoko Watanabe9College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, JapanCancer Chemotherapy Center, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo 135-8550, JapanCollege of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, JapanUnifrutti Tropical Philippines, Inc., Km. 15, Panacan, Davao City 8000, PhilippinesBaCaDM Project of College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, JapanUnifrutti Japan Corporation, 1-11-1 Marunouchi, Chiyoda-Ku, Tokyo 100-6217, JapanUnifrutti Japan Corporation, 1-11-1 Marunouchi, Chiyoda-Ku, Tokyo 100-6217, JapanPhilippine Center for Postharvest Development and Mechanization, Science City of Muñoz 3120, PhilippinesDepartment of Biology, Central Luzon State University, Science City of Muñoz 3120, PhilippinesCollege of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Machida, Tokyo 194-8610, JapanThe pathogen causing Fusarium wilt in banana is reported to be <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (FOC). In 2019, wilt symptoms in banana plants (cultivar: Cavendish) in the Philippines were detected, i.e., the yellowing of the leaves and discoloration of the pseudostem and vascular tissue. The fungus isolated from the vascular tissue was found to be pathogenic to Cavendish bananas and was identified as a new species, <i>F. mindanaoense</i>, belonging to the <i>F. fujikuroi</i> species complex (FFSC); species classification was assessed using molecular phylogenetic analyses based on the <i>tef1</i>, <i>tub2</i>, <i>cmdA</i>, <i>rpb1</i>, and <i>rpb2</i> genes and morphological analyses. A reciprocal blast search using genomic data revealed that this fungus exclusively included the <i>Secreted in Xylem 6</i> (<i>SIX6</i>) gene among the <i>SIX</i> homologs related to pathogenicity; it exhibited a highly conserved amino acid sequence compared with that of species in the FFSC, but not with that of FOC. This was the first report of Fusarium wilt in Cavendish bananas caused by a species of the genus <i>Fusarium</i> other than those in the <i>F. oxysporum</i> species complex.https://www.mdpi.com/2309-608X/9/4/443Fusarium wiltnew species<i>SIX</i> geneplant diseasemultigene phylogeny |
| spellingShingle | Shunsuke Nozawa Yosuke Seto Yoshiki Takata Lalaine Albano Narreto Reynaldo R. Valle Keiju Okui Shigeya Taida Dionisio G. Alvindia Renato G. Reyes Kyoko Watanabe <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex Journal of Fungi Fusarium wilt new species <i>SIX</i> gene plant disease multigene phylogeny |
| title | <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex |
| title_full | <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex |
| title_fullStr | <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex |
| title_full_unstemmed | <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex |
| title_short | <i>Fusarium mindanaoense</i> sp. nov., a New Fusarium Wilt Pathogen of Cavendish Banana from the Philippines Belonging to the <i>F. fujikuroi</i> Species Complex |
| title_sort | i fusarium mindanaoense i sp nov a new fusarium wilt pathogen of cavendish banana from the philippines belonging to the i f fujikuroi i species complex |
| topic | Fusarium wilt new species <i>SIX</i> gene plant disease multigene phylogeny |
| url | https://www.mdpi.com/2309-608X/9/4/443 |
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