Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection
Neuraminidase is an important target for influenza vaccination. In this study, we generated avian influenza VLPs, expressing hemagglutinin (HA), neuraminidase (NA), HA and NA co-expressed (HANA), to evaluate the protective role of NA against a high (10LD<sub>50</sub>) and low (2LD<sub...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2076-0817/10/10/1291 |
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| author | Hae-Ji Kang Ki-Back Chu Keon-Woong Yoon Gi-Deok Eom Jie Mao Min-Ju Kim Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan |
| author_facet | Hae-Ji Kang Ki-Back Chu Keon-Woong Yoon Gi-Deok Eom Jie Mao Min-Ju Kim Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan |
| author_sort | Hae-Ji Kang |
| collection | DOAJ |
| description | Neuraminidase is an important target for influenza vaccination. In this study, we generated avian influenza VLPs, expressing hemagglutinin (HA), neuraminidase (NA), HA and NA co-expressed (HANA), to evaluate the protective role of NA against a high (10LD<sub>50</sub>) and low (2LD<sub>50</sub>) dose of avian influenza virus challenge infections. A single immunization with HANA VLPs elicited the highest level of virus-specific IgG, IgG1, and IgG2a responses from the sera post-vaccination and the lungs post-challenge-infection. Potent antibody-secreting cell responses were observed from the spleens and lungs of HANA-VLP-immunized mice post-challenge-infection. HANA VLPs induced the highest CD4<sup>+</sup> T cell, CD8<sup>+</sup> T cell, and germinal center B cells, while strongly limiting inflammatory cytokine production in the lungs compared to other VLP immunization groups. In correlation with these findings, the lowest bodyweight losses and lung virus titers were observed from HANA VLP immunization, and all of the immunized mice survived irrespective of the challenge dose. Contrastingly, VLPs expressing either HA or NA alone failed to elicit complete protection. These results indicated that NA in VLPs played a critical role in inducing protection against a high dose of the challenge infection. |
| first_indexed | 2024-03-10T06:17:44Z |
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| id | doaj.art-ee32a697a6454ce2bfc780a5563e71a2 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T06:17:44Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-ee32a697a6454ce2bfc780a5563e71a22023-11-22T19:33:44ZengMDPI AGPathogens2076-08172021-10-011010129110.3390/pathogens10101291Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge InfectionHae-Ji Kang0Ki-Back Chu1Keon-Woong Yoon2Gi-Deok Eom3Jie Mao4Min-Ju Kim5Su-Hwa Lee6Eun-Kyung Moon7Fu-Shi Quan8Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaNeuraminidase is an important target for influenza vaccination. In this study, we generated avian influenza VLPs, expressing hemagglutinin (HA), neuraminidase (NA), HA and NA co-expressed (HANA), to evaluate the protective role of NA against a high (10LD<sub>50</sub>) and low (2LD<sub>50</sub>) dose of avian influenza virus challenge infections. A single immunization with HANA VLPs elicited the highest level of virus-specific IgG, IgG1, and IgG2a responses from the sera post-vaccination and the lungs post-challenge-infection. Potent antibody-secreting cell responses were observed from the spleens and lungs of HANA-VLP-immunized mice post-challenge-infection. HANA VLPs induced the highest CD4<sup>+</sup> T cell, CD8<sup>+</sup> T cell, and germinal center B cells, while strongly limiting inflammatory cytokine production in the lungs compared to other VLP immunization groups. In correlation with these findings, the lowest bodyweight losses and lung virus titers were observed from HANA VLP immunization, and all of the immunized mice survived irrespective of the challenge dose. Contrastingly, VLPs expressing either HA or NA alone failed to elicit complete protection. These results indicated that NA in VLPs played a critical role in inducing protection against a high dose of the challenge infection.https://www.mdpi.com/2076-0817/10/10/1291avian influenzavirus-like particleneuraminidaseprotection |
| spellingShingle | Hae-Ji Kang Ki-Back Chu Keon-Woong Yoon Gi-Deok Eom Jie Mao Min-Ju Kim Su-Hwa Lee Eun-Kyung Moon Fu-Shi Quan Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection Pathogens avian influenza virus-like particle neuraminidase protection |
| title | Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection |
| title_full | Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection |
| title_fullStr | Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection |
| title_full_unstemmed | Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection |
| title_short | Neuraminidase in Virus-like Particles Contributes to the Protection against High Dose of Avian Influenza Virus Challenge Infection |
| title_sort | neuraminidase in virus like particles contributes to the protection against high dose of avian influenza virus challenge infection |
| topic | avian influenza virus-like particle neuraminidase protection |
| url | https://www.mdpi.com/2076-0817/10/10/1291 |
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