Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields
The organic material amendment has been proven to change the soil antibiotic resistance genes (ARGs) profile, which may threaten human health through the food chain, but the effects and mechanisms of different organic materials on ARGs in paddy soils are less explored. In this study, a field experim...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1075234/full |
| _version_ | 1797945006907981824 |
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| author | Zongming Li Zongming Li Jupei Shen Fangfang Wang Meihui Wang Meihui Wang Jianlin Shen Jianlin Shen Yong Li Yong Li Qihong Zhu Qihong Zhu Jinshui Wu Jinshui Wu |
| author_facet | Zongming Li Zongming Li Jupei Shen Fangfang Wang Meihui Wang Meihui Wang Jianlin Shen Jianlin Shen Yong Li Yong Li Qihong Zhu Qihong Zhu Jinshui Wu Jinshui Wu |
| author_sort | Zongming Li |
| collection | DOAJ |
| description | The organic material amendment has been proven to change the soil antibiotic resistance genes (ARGs) profile, which may threaten human health through the food chain, but the effects and mechanisms of different organic materials on ARGs in paddy soils are less explored. In this study, a field experiment was set up with the treatments of conventional chemical fertilization (NPK) and common organic material amendment [rice straw (RS), swine manure (SM), and biochar (BC)] to explore the effects and mechanisms. In total, 84 unique ARGs were found across the soil samples with different organic material amendments, and they conferred resistance to the major antibiotic classes. Compared with NPK, SM significantly increased the detected number and relative abundance of ARGs. A higher detected number of ARGs than NPK was observed in BC, whereas BC had a lower relative abundance of ARGs than NPK. Compared with NPK, a detected number decrease was observed in RS, although abundance showed no significant differences. Compared with other treatments, a higher detected number and relative abundance of mobile genetic elements (MGEs) were observed in BC, indicating a higher potential for horizontal gene transfer. There were significantly positive relationships between the relative abundances of total ARGs and MGEs and the bacterial abundance. The network analysis suggested the important role of MGEs and bacterial communities in shaping the ARGs profile. Mantel test and redundancy analysis (RDA) suggested that soil carbon, nitrogen, and C/N were the major chemical drivers of the ARGs profile. The risk of ARGs spreading to the food chain should be considered when applying SM and biochar, which shifted the ARGs and MGEs profiles, respectively. Pre-treatment measures need to be studied to reduce the dissemination of ARGs in paddy fields. |
| first_indexed | 2024-04-10T20:48:35Z |
| format | Article |
| id | doaj.art-ee35ce02d1684b6689388efb1c612f7b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T20:48:35Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ee35ce02d1684b6689388efb1c612f7b2023-01-24T05:39:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10752341075234Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fieldsZongming Li0Zongming Li1Jupei Shen2Fangfang Wang3Meihui Wang4Meihui Wang5Jianlin Shen6Jianlin Shen7Yong Li8Yong Li9Qihong Zhu10Qihong Zhu11Jinshui Wu12Jinshui Wu13Key Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaSchool of Geographical Sciences, Fujian Normal University, Fuzhou, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Agro-Ecological Processes in Subtropical Region and Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Resources and Environment, University of the Chinese Academy of Sciences, Beijing, ChinaThe organic material amendment has been proven to change the soil antibiotic resistance genes (ARGs) profile, which may threaten human health through the food chain, but the effects and mechanisms of different organic materials on ARGs in paddy soils are less explored. In this study, a field experiment was set up with the treatments of conventional chemical fertilization (NPK) and common organic material amendment [rice straw (RS), swine manure (SM), and biochar (BC)] to explore the effects and mechanisms. In total, 84 unique ARGs were found across the soil samples with different organic material amendments, and they conferred resistance to the major antibiotic classes. Compared with NPK, SM significantly increased the detected number and relative abundance of ARGs. A higher detected number of ARGs than NPK was observed in BC, whereas BC had a lower relative abundance of ARGs than NPK. Compared with NPK, a detected number decrease was observed in RS, although abundance showed no significant differences. Compared with other treatments, a higher detected number and relative abundance of mobile genetic elements (MGEs) were observed in BC, indicating a higher potential for horizontal gene transfer. There were significantly positive relationships between the relative abundances of total ARGs and MGEs and the bacterial abundance. The network analysis suggested the important role of MGEs and bacterial communities in shaping the ARGs profile. Mantel test and redundancy analysis (RDA) suggested that soil carbon, nitrogen, and C/N were the major chemical drivers of the ARGs profile. The risk of ARGs spreading to the food chain should be considered when applying SM and biochar, which shifted the ARGs and MGEs profiles, respectively. Pre-treatment measures need to be studied to reduce the dissemination of ARGs in paddy fields.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1075234/fullorganic materialsbiocharantibiotic resistance genesbacterial communitypaddy soil |
| spellingShingle | Zongming Li Zongming Li Jupei Shen Fangfang Wang Meihui Wang Meihui Wang Jianlin Shen Jianlin Shen Yong Li Yong Li Qihong Zhu Qihong Zhu Jinshui Wu Jinshui Wu Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields Frontiers in Microbiology organic materials biochar antibiotic resistance genes bacterial community paddy soil |
| title | Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| title_full | Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| title_fullStr | Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| title_full_unstemmed | Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| title_short | Impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| title_sort | impacts of organic materials amendment on the soil antibiotic resistome in subtropical paddy fields |
| topic | organic materials biochar antibiotic resistance genes bacterial community paddy soil |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1075234/full |
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