Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis
Powdery mildew disease of rubber affects immature green leaves, buds, inflorescences, and other immature tissues of rubber trees, resulting in up to 45% losses in rubber latex yield worldwide. The disease is often controlled by dusting the diseased plants with powdered sulfur, which can have long-te...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00012/full |
| _version_ | 1811275425898299392 |
|---|---|
| author | Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Sehroon Khan Sehroon Khan Siraprapa Brooks Peter E. Mortimer Peter E. Mortimer Samantha C. Karunarathna Samantha C. Karunarathna Jianchu Xu Jianchu Xu Kevin D. Hyde Kevin D. Hyde |
| author_facet | Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Sehroon Khan Sehroon Khan Siraprapa Brooks Peter E. Mortimer Peter E. Mortimer Samantha C. Karunarathna Samantha C. Karunarathna Jianchu Xu Jianchu Xu Kevin D. Hyde Kevin D. Hyde |
| author_sort | Kapila K. Liyanage |
| collection | DOAJ |
| description | Powdery mildew disease of rubber affects immature green leaves, buds, inflorescences, and other immature tissues of rubber trees, resulting in up to 45% losses in rubber latex yield worldwide. The disease is often controlled by dusting the diseased plants with powdered sulfur, which can have long-term negative effects on the environment. Therefore, it is necessary to search for alternative and environmentally friendly control methods for this disease. This study aimed to identify mycoparasites associated with rubber powdery mildew species, and characterize them on the basis of morpho-molecular characteristics and phylogenetic analyses of ITS rDNA regions. We observed that the Ampelomyces fungus parasitizes rubber powdery mildew, and eventually destroys it. Furthermore, on the basis of phylogenetic analyses and morphological characteristics we confirmed that the Ampelomyces mycoparasite isolated from rubber powdery mildew is closely related to other mycohost taxa in the Erysiphe genus. A total of 73 (71 retrieved from GenBank and two obtained from fresh collections of rubber powdery mildew fungi) Ampelomyces spp. were analyzed using ITS rDNA sequences and 153 polymorphic sites were identified through haplotypic analyses. A total of 28 haplotypes (H1–H28) were identified to have a complex network of mutation events. The results from phylogenetic tree constructed on the basis of maximum likelihood analyses, and the haplotype network tree revealed similar relationships of clustering pattern. This work presents the first report on morpho-molecular characterization of Ampelomyces species that are mycoparasites of powdery mildew of Hevea brasiliensis. |
| first_indexed | 2024-04-12T23:37:40Z |
| format | Article |
| id | doaj.art-ee392eba3f444243ae3362ad87a054bf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T23:37:40Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ee392eba3f444243ae3362ad87a054bf2022-12-22T03:12:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01910.3389/fmicb.2018.00012307461Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensisKapila K. Liyanage0Kapila K. Liyanage1Kapila K. Liyanage2Kapila K. Liyanage3Sehroon Khan4Sehroon Khan5Siraprapa Brooks6Peter E. Mortimer7Peter E. Mortimer8Samantha C. Karunarathna9Samantha C. Karunarathna10Jianchu Xu11Jianchu Xu12Kevin D. Hyde13Kevin D. Hyde14Center of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai, ThailandCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaWorld Agroforestry Centre, East and Central Asia, Kunming, ChinaRubber Research Institute of Sri Lanka, Agalawatta, Sri LankaCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaWorld Agroforestry Centre, East and Central Asia, Kunming, ChinaCenter of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai, ThailandCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaWorld Agroforestry Centre, East and Central Asia, Kunming, ChinaCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaWorld Agroforestry Centre, East and Central Asia, Kunming, ChinaCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaWorld Agroforestry Centre, East and Central Asia, Kunming, ChinaCenter of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai, ThailandCenter for Mountain Ecosystem Studies, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaPowdery mildew disease of rubber affects immature green leaves, buds, inflorescences, and other immature tissues of rubber trees, resulting in up to 45% losses in rubber latex yield worldwide. The disease is often controlled by dusting the diseased plants with powdered sulfur, which can have long-term negative effects on the environment. Therefore, it is necessary to search for alternative and environmentally friendly control methods for this disease. This study aimed to identify mycoparasites associated with rubber powdery mildew species, and characterize them on the basis of morpho-molecular characteristics and phylogenetic analyses of ITS rDNA regions. We observed that the Ampelomyces fungus parasitizes rubber powdery mildew, and eventually destroys it. Furthermore, on the basis of phylogenetic analyses and morphological characteristics we confirmed that the Ampelomyces mycoparasite isolated from rubber powdery mildew is closely related to other mycohost taxa in the Erysiphe genus. A total of 73 (71 retrieved from GenBank and two obtained from fresh collections of rubber powdery mildew fungi) Ampelomyces spp. were analyzed using ITS rDNA sequences and 153 polymorphic sites were identified through haplotypic analyses. A total of 28 haplotypes (H1–H28) were identified to have a complex network of mutation events. The results from phylogenetic tree constructed on the basis of maximum likelihood analyses, and the haplotype network tree revealed similar relationships of clustering pattern. This work presents the first report on morpho-molecular characterization of Ampelomyces species that are mycoparasites of powdery mildew of Hevea brasiliensis.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00012/fullErysiphehaplotypemorphologymycohostmycoparasite |
| spellingShingle | Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Kapila K. Liyanage Sehroon Khan Sehroon Khan Siraprapa Brooks Peter E. Mortimer Peter E. Mortimer Samantha C. Karunarathna Samantha C. Karunarathna Jianchu Xu Jianchu Xu Kevin D. Hyde Kevin D. Hyde Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis Frontiers in Microbiology Erysiphe haplotype morphology mycohost mycoparasite |
| title | Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis |
| title_full | Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis |
| title_fullStr | Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis |
| title_full_unstemmed | Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis |
| title_short | Morpho-Molecular Characterization of Two Ampelomyces spp. (Pleosporales) Strains Mycoparasites of Powdery Mildew of Hevea brasiliensis |
| title_sort | morpho molecular characterization of two ampelomyces spp pleosporales strains mycoparasites of powdery mildew of hevea brasiliensis |
| topic | Erysiphe haplotype morphology mycohost mycoparasite |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00012/full |
| work_keys_str_mv | AT kapilakliyanage morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT kapilakliyanage morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT kapilakliyanage morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT kapilakliyanage morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT sehroonkhan morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT sehroonkhan morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT siraprapabrooks morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT peteremortimer morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT peteremortimer morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT samanthackarunarathna morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT samanthackarunarathna morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT jianchuxu morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT jianchuxu morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT kevindhyde morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis AT kevindhyde morphomolecularcharacterizationoftwoampelomycesspppleosporalesstrainsmycoparasitesofpowderymildewofheveabrasiliensis |