Chitinase-1 Activity in Serum of Cats with FIP
Background: Chitotriosidase (chitinase 1 or CHIT1) is secreted by activated macrophages. Macrophages are involved in the pathogenesis of feline infectious peritonitis (FIP). No reports on CHIT1 activity in cats with FIP are available. Objective: To preliminarily investigate the possible changes in s...
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MDPI AG
2023-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1815 |
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| author | Angelica Stranieri Gabriela Ávila Morales Laura Brusasco Saverio Paltrinieri |
| author_facet | Angelica Stranieri Gabriela Ávila Morales Laura Brusasco Saverio Paltrinieri |
| author_sort | Angelica Stranieri |
| collection | DOAJ |
| description | Background: Chitotriosidase (chitinase 1 or CHIT1) is secreted by activated macrophages. Macrophages are involved in the pathogenesis of feline infectious peritonitis (FIP). No reports on CHIT1 activity in cats with FIP are available. Objective: To preliminarily investigate the possible changes in serum CHIT1 activity in cats with FIP. Methods: CHIT1 activity was measured in serum samples from clinically healthy cats (n = 17), cats with FIP (n = 19) and cats with diseases potentially characterized by macrophage activation (n = 20), after a preliminary assessment of the imprecision and linearity of the method. Results: The highest CHIT1 activity was found in cats with FIP, followed by sick cats and clinically healthy cats. The magnitude of the differences between groups was higher than the intra- and inter-assay imprecision of the method (<5% and >57%, respectively). Based on receiver operating characteristic (ROC) curves, CHIT1 may differentiate sick from clinically healthy cats and, to a lesser extent, cats with FIP from cats without FIP. Conclusions: CHIT1 activity may identify sick cats and, within the appropriate clinical context, cats with FIP, although larger and more standardized studies, coupled with additional information on analytical performances of the method, are required to fully explore the diagnostic or prognostic potential of this test for FIP. |
| first_indexed | 2024-03-10T21:53:02Z |
| format | Article |
| id | doaj.art-ee3bcd1afa2b463e8f3af06ef4009e0b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:53:02Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ee3bcd1afa2b463e8f3af06ef4009e0b2023-11-19T13:22:01ZengMDPI AGViruses1999-49152023-08-01159181510.3390/v15091815Chitinase-1 Activity in Serum of Cats with FIPAngelica Stranieri0Gabriela Ávila Morales1Laura Brusasco2Saverio Paltrinieri3Department of Veterinary Medicine and Animal Science, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, University of Milan, 26900 Lodi, ItalyBackground: Chitotriosidase (chitinase 1 or CHIT1) is secreted by activated macrophages. Macrophages are involved in the pathogenesis of feline infectious peritonitis (FIP). No reports on CHIT1 activity in cats with FIP are available. Objective: To preliminarily investigate the possible changes in serum CHIT1 activity in cats with FIP. Methods: CHIT1 activity was measured in serum samples from clinically healthy cats (n = 17), cats with FIP (n = 19) and cats with diseases potentially characterized by macrophage activation (n = 20), after a preliminary assessment of the imprecision and linearity of the method. Results: The highest CHIT1 activity was found in cats with FIP, followed by sick cats and clinically healthy cats. The magnitude of the differences between groups was higher than the intra- and inter-assay imprecision of the method (<5% and >57%, respectively). Based on receiver operating characteristic (ROC) curves, CHIT1 may differentiate sick from clinically healthy cats and, to a lesser extent, cats with FIP from cats without FIP. Conclusions: CHIT1 activity may identify sick cats and, within the appropriate clinical context, cats with FIP, although larger and more standardized studies, coupled with additional information on analytical performances of the method, are required to fully explore the diagnostic or prognostic potential of this test for FIP.https://www.mdpi.com/1999-4915/15/9/1815CHIT1inflammationfeline infectious peritonitismacrophages |
| spellingShingle | Angelica Stranieri Gabriela Ávila Morales Laura Brusasco Saverio Paltrinieri Chitinase-1 Activity in Serum of Cats with FIP Viruses CHIT1 inflammation feline infectious peritonitis macrophages |
| title | Chitinase-1 Activity in Serum of Cats with FIP |
| title_full | Chitinase-1 Activity in Serum of Cats with FIP |
| title_fullStr | Chitinase-1 Activity in Serum of Cats with FIP |
| title_full_unstemmed | Chitinase-1 Activity in Serum of Cats with FIP |
| title_short | Chitinase-1 Activity in Serum of Cats with FIP |
| title_sort | chitinase 1 activity in serum of cats with fip |
| topic | CHIT1 inflammation feline infectious peritonitis macrophages |
| url | https://www.mdpi.com/1999-4915/15/9/1815 |
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