Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition
Soil enzymes play important roles in soil C and nutrient cycling. However, the effects of N addition on soil enzyme kinetics and the underlying mechanisms remain unclear. Thus, we aimed to determine the effects of short-term N addition on the soil properties, microbial properties, maximum reaction r...
| Huvudupphovsmän: | , , , , , , , , , |
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| Materialtyp: | Artikel |
| Språk: | English |
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Elsevier
2024-05-01
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| Serie: | Geoderma |
| Ämnen: | |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S0016706124001149 |
| _version_ | 1827151877465178112 |
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| author | Xiaoqing Zhang Quanxin Zeng Xiaochun Yuan Qiufang Zhang Jiacong Zhou Min Xu Hao Sun Linna Chen Xiaoli Gao Yuehmin Chen |
| author_facet | Xiaoqing Zhang Quanxin Zeng Xiaochun Yuan Qiufang Zhang Jiacong Zhou Min Xu Hao Sun Linna Chen Xiaoli Gao Yuehmin Chen |
| author_sort | Xiaoqing Zhang |
| collection | DOAJ |
| description | Soil enzymes play important roles in soil C and nutrient cycling. However, the effects of N addition on soil enzyme kinetics and the underlying mechanisms remain unclear. Thus, we aimed to determine the effects of short-term N addition on the soil properties, microbial properties, maximum reaction rate (Vm, which is attained at saturating substrate concentrations), and Michaelis constant (Km, where a high Km indicates low substrate affinity) of microbial C- (β-1,4-glucosidase and cellobiohydrolase), N- (β-1,4-N-acetylglucosaminidase and L-leucine aminopeptidase), and P-degrading (acid phosphatase and alkaline phosphatase) enzymes in subtropical coniferous (Pinus taiwanensis) and broadleaf (Castanopsis faberi) forests. In the broadleaf forest, N addition increased the Vm and substrate-binding affinities (decline in Km) of C- and P-degrading enzymes by triggering a P deficiency response in microorganisms (i.e., increased microbial biomass N:P ratio). These findings indicate that the soil enzyme kinetics followed the optimal foraging strategy in response to N addition. Moreover, N addition reduced the proportion of complex organic molecules in dissolved organic matter (DOM; e.g., reduced abundance of humic-like fluorophores and humification index), suggesting that N addition increased soil DOM quality and thus increased the affinities of C-degrading enzymes. N addition increased the abundance of Acidobacteria and Chloroflexi but reduced the abundance of Proteobacteria and Rhizobiales, indicating a shift in microbial community toward efficient P acquisition. N addition affected bacterial composition and thus indirectly influenced N- and P-degrading enzymes. In the coniferous forest, N addition significantly increased the Vm of C-degrading enzymes but did not change other enzyme kinetics, which could be partly attributed to the unchanged N availability and microbial properties. Collectively, our findings provide insights into the relationship between enzyme kinetics, DOM quality, and microbial properties, which are important for predicting soil nutrient cycling and parameterizing models of C cycling under N deposition. |
| first_indexed | 2025-03-20T21:56:57Z |
| format | Article |
| id | doaj.art-ee3d9a1e7dea4652a27a2e9c8f74f74f |
| institution | Directory Open Access Journal |
| issn | 1872-6259 |
| language | English |
| last_indexed | 2025-03-20T21:56:57Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Geoderma |
| spelling | doaj.art-ee3d9a1e7dea4652a27a2e9c8f74f74f2024-08-10T05:24:37ZengElsevierGeoderma1872-62592024-05-01445116885Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen additionXiaoqing Zhang0Quanxin Zeng1Xiaochun Yuan2Qiufang Zhang3Jiacong Zhou4Min Xu5Hao Sun6Linna Chen7Xiaoli Gao8Yuehmin Chen9School of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaCollege of Tourism, Wuyi University, Wuyishan 354300, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaState Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an 710061, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, ChinaSchool of Tourism, Xinyang Normal University, Xinyang 464000, ChinaSchool of Geographical Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350007, China; Corresponding author at: School of Geographical Science, Fujian Normal University, Fuzhou 350007, China.Soil enzymes play important roles in soil C and nutrient cycling. However, the effects of N addition on soil enzyme kinetics and the underlying mechanisms remain unclear. Thus, we aimed to determine the effects of short-term N addition on the soil properties, microbial properties, maximum reaction rate (Vm, which is attained at saturating substrate concentrations), and Michaelis constant (Km, where a high Km indicates low substrate affinity) of microbial C- (β-1,4-glucosidase and cellobiohydrolase), N- (β-1,4-N-acetylglucosaminidase and L-leucine aminopeptidase), and P-degrading (acid phosphatase and alkaline phosphatase) enzymes in subtropical coniferous (Pinus taiwanensis) and broadleaf (Castanopsis faberi) forests. In the broadleaf forest, N addition increased the Vm and substrate-binding affinities (decline in Km) of C- and P-degrading enzymes by triggering a P deficiency response in microorganisms (i.e., increased microbial biomass N:P ratio). These findings indicate that the soil enzyme kinetics followed the optimal foraging strategy in response to N addition. Moreover, N addition reduced the proportion of complex organic molecules in dissolved organic matter (DOM; e.g., reduced abundance of humic-like fluorophores and humification index), suggesting that N addition increased soil DOM quality and thus increased the affinities of C-degrading enzymes. N addition increased the abundance of Acidobacteria and Chloroflexi but reduced the abundance of Proteobacteria and Rhizobiales, indicating a shift in microbial community toward efficient P acquisition. N addition affected bacterial composition and thus indirectly influenced N- and P-degrading enzymes. In the coniferous forest, N addition significantly increased the Vm of C-degrading enzymes but did not change other enzyme kinetics, which could be partly attributed to the unchanged N availability and microbial properties. Collectively, our findings provide insights into the relationship between enzyme kinetics, DOM quality, and microbial properties, which are important for predicting soil nutrient cycling and parameterizing models of C cycling under N deposition.http://www.sciencedirect.com/science/article/pii/S0016706124001149Forest typesDissolved organic matterP deficiencySoil enzyme kinetics |
| spellingShingle | Xiaoqing Zhang Quanxin Zeng Xiaochun Yuan Qiufang Zhang Jiacong Zhou Min Xu Hao Sun Linna Chen Xiaoli Gao Yuehmin Chen Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition Geoderma Forest types Dissolved organic matter P deficiency Soil enzyme kinetics |
| title | Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition |
| title_full | Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition |
| title_fullStr | Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition |
| title_full_unstemmed | Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition |
| title_short | Soil dissolved organic matter quality and bacterial community composition regulate the substrate-binding affinity of hydrolytic enzymes under short-term nitrogen addition |
| title_sort | soil dissolved organic matter quality and bacterial community composition regulate the substrate binding affinity of hydrolytic enzymes under short term nitrogen addition |
| topic | Forest types Dissolved organic matter P deficiency Soil enzyme kinetics |
| url | http://www.sciencedirect.com/science/article/pii/S0016706124001149 |
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