Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides
Endophytic microorganisms (EMs) have recently attracted interest for applications in plant protection, mainly due to their bioactive compound-producing capacity. Therefore, we underwent the task of isolating olive tree EMs and investigating their bioactivity against the devastating pathogen <i>...
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MDPI AG
2021-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/12/833 |
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| author | Evgenia-Anna Papadopoulou Apostolis Angelis Lemonia Antoniadi Konstantinos A. Aliferis Alexios-Leandros Skaltsounis |
| author_facet | Evgenia-Anna Papadopoulou Apostolis Angelis Lemonia Antoniadi Konstantinos A. Aliferis Alexios-Leandros Skaltsounis |
| author_sort | Evgenia-Anna Papadopoulou |
| collection | DOAJ |
| description | Endophytic microorganisms (EMs) have recently attracted interest for applications in plant protection, mainly due to their bioactive compound-producing capacity. Therefore, we underwent the task of isolating olive tree EMs and investigating their bioactivity against the devastating pathogen <i>Colletotrichum acutatum</i>. Several EMs were isolated; however, the <i>Bacillus</i> sp. PTA13 isolate exhibited the highest toxicity to the phytopathogen. Bacteria of the genus <i>Bacillus</i> exhibit superior bioactive metabolite-producing capacity, with the lipopeptides (LPs) of surfactin, iturin, and fengycin groups being the most studied. A total LP extract and several fractions were obtained, and their bioactivity was assessed against <i>C. acutatum</i> strains. LPs of the major surfactin, iturin, and fengycin groups and the minor gageotetrin and bacilotetrin groups were annotated. The results confirmed the bioactivity of the major LPs, with fengycins being the most fungitoxic. Interestingly, the minor LP fraction exhibited selective toxicity to the fungicide-resistant <i>C. acutatum</i> isolate, an observation that highlights the significance of our approach to comprehensively mine the total LP extract. This work represents a proof of concept of the exploitation of EMs in customized olive tree plant protection and aligns well with strategies that focus on the sustainability and safety of food production via the development of next-generation plant protection products. |
| first_indexed | 2024-03-10T03:35:41Z |
| format | Article |
| id | doaj.art-ee3e6c5e4b2945f08c05b7600f160619 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T03:35:41Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-ee3e6c5e4b2945f08c05b7600f1606192023-11-23T09:31:33ZengMDPI AGMetabolites2218-19892021-12-01111283310.3390/metabo11120833Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 LipopeptidesEvgenia-Anna Papadopoulou0Apostolis Angelis1Lemonia Antoniadi2Konstantinos A. Aliferis3Alexios-Leandros Skaltsounis4Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 11855 Athens, GreeceDepartment of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, GreeceDepartment of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, GreeceLaboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 11855 Athens, GreeceDepartment of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, GreeceEndophytic microorganisms (EMs) have recently attracted interest for applications in plant protection, mainly due to their bioactive compound-producing capacity. Therefore, we underwent the task of isolating olive tree EMs and investigating their bioactivity against the devastating pathogen <i>Colletotrichum acutatum</i>. Several EMs were isolated; however, the <i>Bacillus</i> sp. PTA13 isolate exhibited the highest toxicity to the phytopathogen. Bacteria of the genus <i>Bacillus</i> exhibit superior bioactive metabolite-producing capacity, with the lipopeptides (LPs) of surfactin, iturin, and fengycin groups being the most studied. A total LP extract and several fractions were obtained, and their bioactivity was assessed against <i>C. acutatum</i> strains. LPs of the major surfactin, iturin, and fengycin groups and the minor gageotetrin and bacilotetrin groups were annotated. The results confirmed the bioactivity of the major LPs, with fengycins being the most fungitoxic. Interestingly, the minor LP fraction exhibited selective toxicity to the fungicide-resistant <i>C. acutatum</i> isolate, an observation that highlights the significance of our approach to comprehensively mine the total LP extract. This work represents a proof of concept of the exploitation of EMs in customized olive tree plant protection and aligns well with strategies that focus on the sustainability and safety of food production via the development of next-generation plant protection products.https://www.mdpi.com/2218-1989/11/12/833countercurrent partition chromatographyendophytic microorganismsfengycinsiturinsnatural productsolive anthracnose |
| spellingShingle | Evgenia-Anna Papadopoulou Apostolis Angelis Lemonia Antoniadi Konstantinos A. Aliferis Alexios-Leandros Skaltsounis Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides Metabolites countercurrent partition chromatography endophytic microorganisms fengycins iturins natural products olive anthracnose |
| title | Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides |
| title_full | Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides |
| title_fullStr | Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides |
| title_full_unstemmed | Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides |
| title_short | Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte <i>Bacillus</i> sp. PTA13 Lipopeptides |
| title_sort | discovering the next generation plant protection products a proof of concept via the isolation and bioactivity assessment of the olive tree endophyte i bacillus i sp pta13 lipopeptides |
| topic | countercurrent partition chromatography endophytic microorganisms fengycins iturins natural products olive anthracnose |
| url | https://www.mdpi.com/2218-1989/11/12/833 |
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