Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam
A cationic derivative of pullulan was obtained by grafting reaction and used together with dextran sulfate to form polysaccharide-based nanohydrogel cross-linked via electrostatic interactions between polyions. Due to the polycation-polyanion interactions nanohydrogel particles were formed instantly...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/11/12/622 |
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| author | Dorota Lachowicz Przemyslaw Mielczarek Roma Wirecka Katarzyna Berent Anna Karewicz Michał Szuwarzyński Szczepan Zapotoczny |
| author_facet | Dorota Lachowicz Przemyslaw Mielczarek Roma Wirecka Katarzyna Berent Anna Karewicz Michał Szuwarzyński Szczepan Zapotoczny |
| author_sort | Dorota Lachowicz |
| collection | DOAJ |
| description | A cationic derivative of pullulan was obtained by grafting reaction and used together with dextran sulfate to form polysaccharide-based nanohydrogel cross-linked via electrostatic interactions between polyions. Due to the polycation-polyanion interactions nanohydrogel particles were formed instantly and spontaneously in water. The nanoparticles were colloidally stable and their size and surface charge could be controlled by the polycation/polyanion ratio. The morphology of the obtained particles was visualized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). The resulting structures were spherical, with hydrodynamic diameters in the range of 100−150 nm. The binding constant (<i>K<sub>a</sub></i>) of a model drug, piroxicam, to the cationic pullulan (C-PUL) was determined by spectrophotometric measurements. The value of <i>K<sub>a</sub></i> was calculated according to the Benesi—Hildebrand equation to be (3.6 ± 0.2) × 10<sup>3</sup> M<sup>−1</sup>. After binding to cationic pullulan, piroxicam was effectively entrapped inside the nanohydrogel particles and released in a controlled way. The obtained system was efficiently taken up by cells and was shown to be biocompatible. |
| first_indexed | 2024-04-14T02:07:33Z |
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| id | doaj.art-ee3edf60e2b8426cb3f66f478d1213cb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-14T02:07:33Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-ee3edf60e2b8426cb3f66f478d1213cb2022-12-22T02:18:38ZengMDPI AGPharmaceutics1999-49232019-11-01111262210.3390/pharmaceutics11120622pharmaceutics11120622Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of PiroxicamDorota Lachowicz0Przemyslaw Mielczarek1Roma Wirecka2Katarzyna Berent3Anna Karewicz4Michał Szuwarzyński5Szczepan Zapotoczny6Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, PolandDepartment of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, PolandAcademic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, PolandAcademic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandAcademic Centre for Materials and Nanotechnology, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Krakow, PolandFaculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandA cationic derivative of pullulan was obtained by grafting reaction and used together with dextran sulfate to form polysaccharide-based nanohydrogel cross-linked via electrostatic interactions between polyions. Due to the polycation-polyanion interactions nanohydrogel particles were formed instantly and spontaneously in water. The nanoparticles were colloidally stable and their size and surface charge could be controlled by the polycation/polyanion ratio. The morphology of the obtained particles was visualized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). The resulting structures were spherical, with hydrodynamic diameters in the range of 100−150 nm. The binding constant (<i>K<sub>a</sub></i>) of a model drug, piroxicam, to the cationic pullulan (C-PUL) was determined by spectrophotometric measurements. The value of <i>K<sub>a</sub></i> was calculated according to the Benesi—Hildebrand equation to be (3.6 ± 0.2) × 10<sup>3</sup> M<sup>−1</sup>. After binding to cationic pullulan, piroxicam was effectively entrapped inside the nanohydrogel particles and released in a controlled way. The obtained system was efficiently taken up by cells and was shown to be biocompatible.https://www.mdpi.com/1999-4923/11/12/622nanohydrogelself-organizationpiroxicamcationic pullulananionic dextranpolyelectrolyte |
| spellingShingle | Dorota Lachowicz Przemyslaw Mielczarek Roma Wirecka Katarzyna Berent Anna Karewicz Michał Szuwarzyński Szczepan Zapotoczny Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam Pharmaceutics nanohydrogel self-organization piroxicam cationic pullulan anionic dextran polyelectrolyte |
| title | Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam |
| title_full | Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam |
| title_fullStr | Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam |
| title_full_unstemmed | Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam |
| title_short | Nanohydrogels Based on Self-Assembly of Cationic Pullulan and Anionic Dextran Derivatives for Efficient Delivery of Piroxicam |
| title_sort | nanohydrogels based on self assembly of cationic pullulan and anionic dextran derivatives for efficient delivery of piroxicam |
| topic | nanohydrogel self-organization piroxicam cationic pullulan anionic dextran polyelectrolyte |
| url | https://www.mdpi.com/1999-4923/11/12/622 |
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