Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds.
BACKGROUND: The extract from Moringa oleifera seeds is used worldwide, especially in rural areas of developing countries, to treat drinking water. M. oleifera seeds contain the lectins cmol and WSMoL, which are carbohydrate-binding proteins that are able to reduce water turbidity because of their co...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3857229?pdf=render |
| _version_ | 1828300272380674048 |
|---|---|
| author | Larissa Cardoso Corrêa Araújo Jaciana Santos Aguiar Thiago Henrique Napoleão Fernanda Virgínia Barreto Mota André Luiz Souza Barros Maiara Celine Moura Marília Cavalcanti Coriolano Luana Cassandra Breitenbach Barroso Coelho Teresinha Gonçalves Silva Patrícia Maria Guedes Paiva |
| author_facet | Larissa Cardoso Corrêa Araújo Jaciana Santos Aguiar Thiago Henrique Napoleão Fernanda Virgínia Barreto Mota André Luiz Souza Barros Maiara Celine Moura Marília Cavalcanti Coriolano Luana Cassandra Breitenbach Barroso Coelho Teresinha Gonçalves Silva Patrícia Maria Guedes Paiva |
| author_sort | Larissa Cardoso Corrêa Araújo |
| collection | DOAJ |
| description | BACKGROUND: The extract from Moringa oleifera seeds is used worldwide, especially in rural areas of developing countries, to treat drinking water. M. oleifera seeds contain the lectins cmol and WSMoL, which are carbohydrate-binding proteins that are able to reduce water turbidity because of their coagulant activity. Studies investigating the ability of natural products to damage normal cells are essential for the safe use of these substances. This study evaluated the cytotoxic and anti-inflammatory properties of the aqueous seed extract, the extract used by population to treat water (named diluted seed extract in this work), and the isolated lectins cmol and WSMoL. METHODOLOGY/PRINCIPAL FINDINGS: The data showed that the aqueous seed extract and cmol were potentially cytotoxic to human peripheral blood mononuclear cells, while WSMoL and diluted seed extract were not cytotoxic. The M. oleifera aqueous seed extract and the lectins cmol and WSMoL were weakly/moderately cytotoxic to the NCI-H292, HT-29 and HEp-2 cancer cell lines and were not hemolytic to murine erythrocytes. Evaluation of acute toxicity in mice revealed that the aqueous seed extract (2.000 mg/kg) did not cause systemic toxicity. The aqueous seed extract, cmol and WSMoL (6.25 µg/mL) and diluted seed extract at 50 µg/mL exhibited anti-inflammatory activity on lipopolyssaccharide-stimulated murine macrophages by regulating the production of nitric oxide, TNF-α and IL-1β. The aqueous seed extract reduced leukocyte migration in a mouse model of carrageenan-induced pleurisy; the myeloperoxidase activity and nitric oxide, TNF-α and IL-1β levels were similarly reduced. Histological analysis of the lungs showed that the extract reduced the number of leukocytes. CONCLUSION/SIGNIFICANCE: This study shows that the extract prepared according to folk use and WSMoL may be non-toxic to mammalian cells; however, the aqueous seed extract and cmol may be cytotoxic to immune cells which may explain the immunosuppressive potential of the extract. |
| first_indexed | 2024-04-13T13:07:58Z |
| format | Article |
| id | doaj.art-ee42b29a07bf4b61b08b8a3abbee853d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:07:58Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ee42b29a07bf4b61b08b8a3abbee853d2022-12-22T02:45:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8197310.1371/journal.pone.0081973Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds.Larissa Cardoso Corrêa AraújoJaciana Santos AguiarThiago Henrique NapoleãoFernanda Virgínia Barreto MotaAndré Luiz Souza BarrosMaiara Celine MouraMarília Cavalcanti CoriolanoLuana Cassandra Breitenbach Barroso CoelhoTeresinha Gonçalves SilvaPatrícia Maria Guedes PaivaBACKGROUND: The extract from Moringa oleifera seeds is used worldwide, especially in rural areas of developing countries, to treat drinking water. M. oleifera seeds contain the lectins cmol and WSMoL, which are carbohydrate-binding proteins that are able to reduce water turbidity because of their coagulant activity. Studies investigating the ability of natural products to damage normal cells are essential for the safe use of these substances. This study evaluated the cytotoxic and anti-inflammatory properties of the aqueous seed extract, the extract used by population to treat water (named diluted seed extract in this work), and the isolated lectins cmol and WSMoL. METHODOLOGY/PRINCIPAL FINDINGS: The data showed that the aqueous seed extract and cmol were potentially cytotoxic to human peripheral blood mononuclear cells, while WSMoL and diluted seed extract were not cytotoxic. The M. oleifera aqueous seed extract and the lectins cmol and WSMoL were weakly/moderately cytotoxic to the NCI-H292, HT-29 and HEp-2 cancer cell lines and were not hemolytic to murine erythrocytes. Evaluation of acute toxicity in mice revealed that the aqueous seed extract (2.000 mg/kg) did not cause systemic toxicity. The aqueous seed extract, cmol and WSMoL (6.25 µg/mL) and diluted seed extract at 50 µg/mL exhibited anti-inflammatory activity on lipopolyssaccharide-stimulated murine macrophages by regulating the production of nitric oxide, TNF-α and IL-1β. The aqueous seed extract reduced leukocyte migration in a mouse model of carrageenan-induced pleurisy; the myeloperoxidase activity and nitric oxide, TNF-α and IL-1β levels were similarly reduced. Histological analysis of the lungs showed that the extract reduced the number of leukocytes. CONCLUSION/SIGNIFICANCE: This study shows that the extract prepared according to folk use and WSMoL may be non-toxic to mammalian cells; however, the aqueous seed extract and cmol may be cytotoxic to immune cells which may explain the immunosuppressive potential of the extract.http://europepmc.org/articles/PMC3857229?pdf=render |
| spellingShingle | Larissa Cardoso Corrêa Araújo Jaciana Santos Aguiar Thiago Henrique Napoleão Fernanda Virgínia Barreto Mota André Luiz Souza Barros Maiara Celine Moura Marília Cavalcanti Coriolano Luana Cassandra Breitenbach Barroso Coelho Teresinha Gonçalves Silva Patrícia Maria Guedes Paiva Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. PLoS ONE |
| title | Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. |
| title_full | Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. |
| title_fullStr | Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. |
| title_full_unstemmed | Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. |
| title_short | Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. |
| title_sort | evaluation of cytotoxic and anti inflammatory activities of extracts and lectins from moringa oleifera seeds |
| url | http://europepmc.org/articles/PMC3857229?pdf=render |
| work_keys_str_mv | AT larissacardosocorreaaraujo evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT jacianasantosaguiar evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT thiagohenriquenapoleao evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT fernandavirginiabarretomota evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT andreluizsouzabarros evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT maiaracelinemoura evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT mariliacavalcanticoriolano evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT luanacassandrabreitenbachbarrosocoelho evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT teresinhagoncalvessilva evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds AT patriciamariaguedespaiva evaluationofcytotoxicandantiinflammatoryactivitiesofextractsandlectinsfrommoringaoleiferaseeds |