Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora
Erwinia amylovora, causative agent of fire blight disease of apple and pear trees, has evolved to use small RNAs for post-transcriptional regulation of virulence traits important for disease development. The sRNA ArcZ regulates several virulence traits, and to better understand its roles, we conduct...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02775/full |
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| author | Jeffrey K. Schachterle Jeffrey K. Schachterle Daphne M. Onsay George W. Sundin George W. Sundin |
| author_facet | Jeffrey K. Schachterle Jeffrey K. Schachterle Daphne M. Onsay George W. Sundin George W. Sundin |
| author_sort | Jeffrey K. Schachterle |
| collection | DOAJ |
| description | Erwinia amylovora, causative agent of fire blight disease of apple and pear trees, has evolved to use small RNAs for post-transcriptional regulation of virulence traits important for disease development. The sRNA ArcZ regulates several virulence traits, and to better understand its roles, we conducted a transcriptomic comparison of wild-type and ΔarcZ mutant E. amylovora. We found that ArcZ regulates multiple cellular processes including genes encoding enzymes involved in mitigating the threat of reactive oxygen species (katA, tpx, osmC), and that the ΔarcZ mutant has reduced catalase activity and is more susceptible to exogenous hydrogen peroxide. We quantified hydrogen peroxide production by apple leaves inoculated with E. amylovora and found that the while wild-type E. amylovora cells produce enough catalase to cope with defense peroxide, the ΔarcZ mutant is likely limited in virulence because of inability to cope with peroxide levels in host leaves. We further found that the ArcZ regulon overlaps significantly with the regulons of transcription factors involved in oxidative sensing including Fnr and ArcA. In addition, we show that ArcZ regulates arcA at the post-transcriptional level suggesting a role for this system in mediating adaptations to oxidative state, especially during disease development. |
| first_indexed | 2024-12-12T02:01:23Z |
| format | Article |
| id | doaj.art-ee43967dfb3c45d7a4c747dbb4bd223e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T02:01:23Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ee43967dfb3c45d7a4c747dbb4bd223e2022-12-22T00:42:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02775473947Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovoraJeffrey K. Schachterle0Jeffrey K. Schachterle1Daphne M. Onsay2George W. Sundin3George W. Sundin4Genetics Graduate Program, Michigan State University, East Lansing, MI, United StatesDepartment of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, United StatesDepartment of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, United StatesGenetics Graduate Program, Michigan State University, East Lansing, MI, United StatesDepartment of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, United StatesErwinia amylovora, causative agent of fire blight disease of apple and pear trees, has evolved to use small RNAs for post-transcriptional regulation of virulence traits important for disease development. The sRNA ArcZ regulates several virulence traits, and to better understand its roles, we conducted a transcriptomic comparison of wild-type and ΔarcZ mutant E. amylovora. We found that ArcZ regulates multiple cellular processes including genes encoding enzymes involved in mitigating the threat of reactive oxygen species (katA, tpx, osmC), and that the ΔarcZ mutant has reduced catalase activity and is more susceptible to exogenous hydrogen peroxide. We quantified hydrogen peroxide production by apple leaves inoculated with E. amylovora and found that the while wild-type E. amylovora cells produce enough catalase to cope with defense peroxide, the ΔarcZ mutant is likely limited in virulence because of inability to cope with peroxide levels in host leaves. We further found that the ArcZ regulon overlaps significantly with the regulons of transcription factors involved in oxidative sensing including Fnr and ArcA. In addition, we show that ArcZ regulates arcA at the post-transcriptional level suggesting a role for this system in mediating adaptations to oxidative state, especially during disease development.https://www.frontiersin.org/article/10.3389/fmicb.2019.02775/fullsRNAArcZcatalasefire blightErwinia amylovoraperoxide |
| spellingShingle | Jeffrey K. Schachterle Jeffrey K. Schachterle Daphne M. Onsay George W. Sundin George W. Sundin Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora Frontiers in Microbiology sRNA ArcZ catalase fire blight Erwinia amylovora peroxide |
| title | Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora |
| title_full | Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora |
| title_fullStr | Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora |
| title_full_unstemmed | Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora |
| title_short | Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora |
| title_sort | small rna arcz regulates oxidative stress response genes and regulons in erwinia amylovora |
| topic | sRNA ArcZ catalase fire blight Erwinia amylovora peroxide |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02775/full |
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