Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis
The NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllost...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-03-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8716.pdf |
| _version_ | 1797420592689840128 |
|---|---|
| author | Lihua Xie Miaomiao Cai Xiangyu Li Huifang Zheng Yali Xie Zhanchao Cheng Yucong Bai Juan Li Shaohua Mu Jian Gao |
| author_facet | Lihua Xie Miaomiao Cai Xiangyu Li Huifang Zheng Yali Xie Zhanchao Cheng Yucong Bai Juan Li Shaohua Mu Jian Gao |
| author_sort | Lihua Xie |
| collection | DOAJ |
| description | The NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllostachys edulis). PheNAC3 belong to the NAC1 subgroup and has a conserved NAC domain on the N-terminus, which with 88.74% similarity to ONAC011 protein. PheNAC3 localized in the nucleus and exhibited transactivation activity. PheNAC3 was upregulated during the process of senescence of leaves and detected shoots. PheNAC3 was also induced by ABA, MeJA, NaCl and darkness, but it had no remarkable response to PEG and SA treatments. Overexpression of PheNAC3 could cause precocious senescence in Arabidopsis. Transgenic Arabidopsis displayed faster seed germination, better seedling growth, and a higher survival rate than the wild-type under salt or drought stress conditions. Moreover, AtSAG12 associated with senescence and AtRD29A and AtRD29b related to ABA were upregulated by PheNAC3 overexpression, but AtCAB was inhibited. These findings show that PheNAC3 may participate in leaf senescence and play critical roles in the salt and drought stress response. |
| first_indexed | 2024-03-09T07:04:39Z |
| format | Article |
| id | doaj.art-ee483a433a3a4b128b0459526b75f548 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:04:39Z |
| publishDate | 2020-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ee483a433a3a4b128b0459526b75f5482023-12-03T09:45:56ZengPeerJ Inc.PeerJ2167-83592020-03-018e871610.7717/peerj.8716Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in ArabidopsisLihua Xie0Miaomiao Cai1Xiangyu Li2Huifang Zheng3Yali Xie4Zhanchao Cheng5Yucong Bai6Juan Li7Shaohua Mu8Jian Gao9Key Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaKey Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, ChinaThe NAC family is one of the largest transcription factor families unique to plants, which regulates the growth and development, biotic and abiotic stress responses, and maturation and senescence in plants. In this study, PheNAC3, a NAC gene, was isolated and characterized from moso bamboo (Phyllostachys edulis). PheNAC3 belong to the NAC1 subgroup and has a conserved NAC domain on the N-terminus, which with 88.74% similarity to ONAC011 protein. PheNAC3 localized in the nucleus and exhibited transactivation activity. PheNAC3 was upregulated during the process of senescence of leaves and detected shoots. PheNAC3 was also induced by ABA, MeJA, NaCl and darkness, but it had no remarkable response to PEG and SA treatments. Overexpression of PheNAC3 could cause precocious senescence in Arabidopsis. Transgenic Arabidopsis displayed faster seed germination, better seedling growth, and a higher survival rate than the wild-type under salt or drought stress conditions. Moreover, AtSAG12 associated with senescence and AtRD29A and AtRD29b related to ABA were upregulated by PheNAC3 overexpression, but AtCAB was inhibited. These findings show that PheNAC3 may participate in leaf senescence and play critical roles in the salt and drought stress response.https://peerj.com/articles/8716.pdfNAC transcription factorsSenescenceAbiotic stressMoso bambooOverexpressionTransgenic Arabidopsis |
| spellingShingle | Lihua Xie Miaomiao Cai Xiangyu Li Huifang Zheng Yali Xie Zhanchao Cheng Yucong Bai Juan Li Shaohua Mu Jian Gao Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis PeerJ NAC transcription factors Senescence Abiotic stress Moso bamboo Overexpression Transgenic Arabidopsis |
| title | Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis |
| title_full | Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis |
| title_fullStr | Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis |
| title_full_unstemmed | Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis |
| title_short | Overexpression of PheNAC3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in Arabidopsis |
| title_sort | overexpression of phenac3 from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in arabidopsis |
| topic | NAC transcription factors Senescence Abiotic stress Moso bamboo Overexpression Transgenic Arabidopsis |
| url | https://peerj.com/articles/8716.pdf |
| work_keys_str_mv | AT lihuaxie overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT miaomiaocai overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT xiangyuli overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT huifangzheng overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT yalixie overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT zhanchaocheng overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT yucongbai overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT juanli overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT shaohuamu overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis AT jiangao overexpressionofphenac3frommosobamboopromotesleafsenescenceandenhancesabioticstresstoleranceinarabidopsis |