Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke
ABSTRACT It is important to understand the microbial features of the cerebral thrombus and its clinical relevance in stroke patients, of which data were scarce. We aimed to investigate the microbial features of cerebral thrombi retrieved via thrombectomy in stroke patients with large vessel occlusio...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-08-01
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| Series: | mBio |
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.01085-22 |
| _version_ | 1811281751824138240 |
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| author | Yu Liao Xiuli Zeng Xiaomei Xie Dan Liang Hongyu Qiao Wence Wang Min Guan Shengming Huang Zhen Jing Xinyi Leng Li’an Huang |
| author_facet | Yu Liao Xiuli Zeng Xiaomei Xie Dan Liang Hongyu Qiao Wence Wang Min Guan Shengming Huang Zhen Jing Xinyi Leng Li’an Huang |
| author_sort | Yu Liao |
| collection | DOAJ |
| description | ABSTRACT It is important to understand the microbial features of the cerebral thrombus and its clinical relevance in stroke patients, of which data were scarce. We aimed to investigate the microbial features of cerebral thrombi retrieved via thrombectomy in stroke patients with large vessel occlusion (LVO) and their correlations with 3-month mortality. In a prospective cohort study, thrombus samples were collected during mechanical thrombectomy in LVO stroke patients with successful revascularization at a tertiary hospital. Oral, fecal, and isolated plasma samples were collected within 12 h of admission. The microbial compositions of all samples were compared using 16S rRNA gene amplicon next-generation sequencing. Fluorescent in situ hybridization (FISH) was used to detect bacteria in thrombus samples. The primary outcome was 3-month mortality. Perioperative adverse events (AEs) within 48 h were also recorded. Bacterial DNA was detected in 96.2% of thrombus samples from 104 patients, and clusters of bacterial signals were seen in the thrombi with FISH. Compared with fecal and oral samples, the thrombus microbiota was mainly characterized by excessive enrichment of Proteobacteria, mainly originating from plasma. The bacterial concentrations, dominant bacteria, and distribution patterns differed in thrombi obtained from cardioembolic and large-artery atherosclerotic strokes. Higher abundances of Acinetobacter and Enterobacteriaceae were associated with a higher risk of perioperative AEs, and a higher abundance of Acinetobacter was independently associated with a higher risk of 90-day mortality. This study demonstrated the presence of bacteria in cerebral thrombi retrieved with thrombectomy in LVO strokes, with some bacteria associated with patients’ prognoses. IMPORTANCE In this study, we (i) checked for the presence of bacteria in cerebral thrombi in over 95% of the LVO stroke patients using 16S rRNA sequencing, in contrast with periprocedural control samples that are bacteria negative; (ii) visualized clusters of bacterial signals in the thrombi using FISH; and (iii) cultivated Lactobacillus vaginalis, Bacillus cereus, and Kocuria marina in the bacterial culture of the tissue fragment solution of thrombus aspirates. We found excessive enrichment of Proteobacteria in the thrombi, mainly originating from plasma, as indicated with fast expectation-maximization microbial source tracking (FEAST). Different bacterial concentrations, dominant bacteria, and distribution patterns were found in thrombi obtained from cardioembolic and large-artery atherosclerotic LVO strokes. There was an association between higher abundances of Acinetobacter and Enterobacteriaceae in the thrombi and a higher risk of perioperative adverse events and an association between a higher abundance of Acinetobacter in the thrombi and a higher risk of 90-day mortality. |
| first_indexed | 2024-04-13T01:39:15Z |
| format | Article |
| id | doaj.art-ee48b193c1054bdaa912f092ec59267e |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-04-13T01:39:15Z |
| publishDate | 2022-08-01 |
| publisher | American Society for Microbiology |
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| series | mBio |
| spelling | doaj.art-ee48b193c1054bdaa912f092ec59267e2022-12-22T03:08:15ZengAmerican Society for MicrobiologymBio2150-75112022-08-0113410.1128/mbio.01085-22Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion StrokeYu Liao0Xiuli Zeng1Xiaomei Xie2Dan Liang3Hongyu Qiao4Wence Wang5Min Guan6Shengming Huang7Zhen Jing8Xinyi Leng9Li’an Huang10Department of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition and Regulation, College of Animal Science, South China Agricultural University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaDepartment of Medicine & Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neurology, The First Affiliated Hospital, Jinan University, Guangzhou, ChinaABSTRACT It is important to understand the microbial features of the cerebral thrombus and its clinical relevance in stroke patients, of which data were scarce. We aimed to investigate the microbial features of cerebral thrombi retrieved via thrombectomy in stroke patients with large vessel occlusion (LVO) and their correlations with 3-month mortality. In a prospective cohort study, thrombus samples were collected during mechanical thrombectomy in LVO stroke patients with successful revascularization at a tertiary hospital. Oral, fecal, and isolated plasma samples were collected within 12 h of admission. The microbial compositions of all samples were compared using 16S rRNA gene amplicon next-generation sequencing. Fluorescent in situ hybridization (FISH) was used to detect bacteria in thrombus samples. The primary outcome was 3-month mortality. Perioperative adverse events (AEs) within 48 h were also recorded. Bacterial DNA was detected in 96.2% of thrombus samples from 104 patients, and clusters of bacterial signals were seen in the thrombi with FISH. Compared with fecal and oral samples, the thrombus microbiota was mainly characterized by excessive enrichment of Proteobacteria, mainly originating from plasma. The bacterial concentrations, dominant bacteria, and distribution patterns differed in thrombi obtained from cardioembolic and large-artery atherosclerotic strokes. Higher abundances of Acinetobacter and Enterobacteriaceae were associated with a higher risk of perioperative AEs, and a higher abundance of Acinetobacter was independently associated with a higher risk of 90-day mortality. This study demonstrated the presence of bacteria in cerebral thrombi retrieved with thrombectomy in LVO strokes, with some bacteria associated with patients’ prognoses. IMPORTANCE In this study, we (i) checked for the presence of bacteria in cerebral thrombi in over 95% of the LVO stroke patients using 16S rRNA sequencing, in contrast with periprocedural control samples that are bacteria negative; (ii) visualized clusters of bacterial signals in the thrombi using FISH; and (iii) cultivated Lactobacillus vaginalis, Bacillus cereus, and Kocuria marina in the bacterial culture of the tissue fragment solution of thrombus aspirates. We found excessive enrichment of Proteobacteria in the thrombi, mainly originating from plasma, as indicated with fast expectation-maximization microbial source tracking (FEAST). Different bacterial concentrations, dominant bacteria, and distribution patterns were found in thrombi obtained from cardioembolic and large-artery atherosclerotic LVO strokes. There was an association between higher abundances of Acinetobacter and Enterobacteriaceae in the thrombi and a higher risk of perioperative adverse events and an association between a higher abundance of Acinetobacter in the thrombi and a higher risk of 90-day mortality.https://journals.asm.org/doi/10.1128/mbio.01085-22bacteriaacute ischemic strokemechanical thrombectomy16S sequencingFISH |
| spellingShingle | Yu Liao Xiuli Zeng Xiaomei Xie Dan Liang Hongyu Qiao Wence Wang Min Guan Shengming Huang Zhen Jing Xinyi Leng Li’an Huang Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke mBio bacteria acute ischemic stroke mechanical thrombectomy 16S sequencing FISH |
| title | Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke |
| title_full | Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke |
| title_fullStr | Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke |
| title_full_unstemmed | Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke |
| title_short | Bacterial Signatures of Cerebral Thrombi in Large Vessel Occlusion Stroke |
| title_sort | bacterial signatures of cerebral thrombi in large vessel occlusion stroke |
| topic | bacteria acute ischemic stroke mechanical thrombectomy 16S sequencing FISH |
| url | https://journals.asm.org/doi/10.1128/mbio.01085-22 |
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