In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives
Introduction: Hydroxychloroquine (HDQ) is an antimalarial drug that has also shown its effectiveness in autoimmune diseases. Despite having side effects such as retinopathy, neuromyopathy and controversial cardiac toxicity, HDQ has been presented and now intensively studied for the treatment and pre...
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| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1128382/full |
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| author | Michelle Vanessa Kamga Kapchoup Jürgen Hescheler Filomain Nguemo |
| author_facet | Michelle Vanessa Kamga Kapchoup Jürgen Hescheler Filomain Nguemo |
| author_sort | Michelle Vanessa Kamga Kapchoup |
| collection | DOAJ |
| description | Introduction: Hydroxychloroquine (HDQ) is an antimalarial drug that has also shown its effectiveness in autoimmune diseases. Despite having side effects such as retinopathy, neuromyopathy and controversial cardiac toxicity, HDQ has been presented and now intensively studied for the treatment and prevention of coronavirus disease 2019 (COVID-19). Recent works revealed both beneficial and toxic effects during HDQ treatment. The cardiotoxic profile of HDQ remains unclear and identifying risk factors is challenging.Methods: Here, we used well-established cell-cultured to study the cytotoxic effect of HDQ, mouse induced pluripotent stem cells (miPSC) and their cardiomyocytes (CMs) derivatives were exposed to different concentrations of HDQ. Cell colony morphology was assessed by microscopy whereas cell viability was measured by flow cytometry and impedance-based methods. The effect of HDQ on beating activity of mouse and human induced pluripotent stem cell-derived CMs (miPSC-CMs and hiPSC-CMs, respectively) and mouse embryonic stem cell-derived CMs (mESC-CMs) were captured by the xCELLigence RTCA and microelectrode array (MEA) systems.Results and discussion: Our results revealed that 20 µM of HDQ promotes proliferation of stem cells used suggesting that if appropriately monitored, HDQ may have a cardioprotective effect and may also represent a possible candidate for tissue repair. In addition, the field potential signals revealed that higher doses of this medication caused bradycardia that could be reversed with a higher concentration of ß-adrenergic agonist, Isoproterenol (Iso). On the contrary, HDQ caused an increase in the beating rate of hiPSC-CMs, which was further helped upon application of Isoproterenol (Iso) suggesting that HDQ and Iso may also work synergistically. These results indicate that HDQ is potentially toxic at high concentrations and can modulate the beating activity of cardiomyocytes. Moreover, HDQ could have a synergistic inotropic effect with isoproterenol on cardiac cells. |
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| id | doaj.art-ee4f0618b32a419c96e630fc015a53fb |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-13T00:07:17Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-ee4f0618b32a419c96e630fc015a53fb2023-07-13T00:13:44ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-07-011410.3389/fphar.2023.11283821128382In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivativesMichelle Vanessa Kamga KapchoupJürgen HeschelerFilomain NguemoIntroduction: Hydroxychloroquine (HDQ) is an antimalarial drug that has also shown its effectiveness in autoimmune diseases. Despite having side effects such as retinopathy, neuromyopathy and controversial cardiac toxicity, HDQ has been presented and now intensively studied for the treatment and prevention of coronavirus disease 2019 (COVID-19). Recent works revealed both beneficial and toxic effects during HDQ treatment. The cardiotoxic profile of HDQ remains unclear and identifying risk factors is challenging.Methods: Here, we used well-established cell-cultured to study the cytotoxic effect of HDQ, mouse induced pluripotent stem cells (miPSC) and their cardiomyocytes (CMs) derivatives were exposed to different concentrations of HDQ. Cell colony morphology was assessed by microscopy whereas cell viability was measured by flow cytometry and impedance-based methods. The effect of HDQ on beating activity of mouse and human induced pluripotent stem cell-derived CMs (miPSC-CMs and hiPSC-CMs, respectively) and mouse embryonic stem cell-derived CMs (mESC-CMs) were captured by the xCELLigence RTCA and microelectrode array (MEA) systems.Results and discussion: Our results revealed that 20 µM of HDQ promotes proliferation of stem cells used suggesting that if appropriately monitored, HDQ may have a cardioprotective effect and may also represent a possible candidate for tissue repair. In addition, the field potential signals revealed that higher doses of this medication caused bradycardia that could be reversed with a higher concentration of ß-adrenergic agonist, Isoproterenol (Iso). On the contrary, HDQ caused an increase in the beating rate of hiPSC-CMs, which was further helped upon application of Isoproterenol (Iso) suggesting that HDQ and Iso may also work synergistically. These results indicate that HDQ is potentially toxic at high concentrations and can modulate the beating activity of cardiomyocytes. Moreover, HDQ could have a synergistic inotropic effect with isoproterenol on cardiac cells.https://www.frontiersin.org/articles/10.3389/fphar.2023.1128382/fullhydroxychloroquinestem cellsproliferationdifferentiationcardiotoxicity |
| spellingShingle | Michelle Vanessa Kamga Kapchoup Jürgen Hescheler Filomain Nguemo In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives Frontiers in Pharmacology hydroxychloroquine stem cells proliferation differentiation cardiotoxicity |
| title | In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| title_full | In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| title_fullStr | In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| title_full_unstemmed | In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| title_short | In vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| title_sort | in vitro effect of hydroxychloroquine on pluripotent stem cells and their cardiomyocytes derivatives |
| topic | hydroxychloroquine stem cells proliferation differentiation cardiotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1128382/full |
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