Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development
Background/Aims: MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research has indicated that miR29b participates in DNA methylation by regulating DNA methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern...
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| Format: | Article |
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Cell Physiol Biochem Press GmbH & Co KG
2015-02-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/373942 |
| _version_ | 1819204145245061120 |
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| author | Junqiang Zhang Ying Wang Xiaoguang Liu Shenglin Jiang Chun Zhao Rong Shen Xirong Guo Xiufeng Ling Chang Liu |
| author_facet | Junqiang Zhang Ying Wang Xiaoguang Liu Shenglin Jiang Chun Zhao Rong Shen Xirong Guo Xiufeng Ling Chang Liu |
| author_sort | Junqiang Zhang |
| collection | DOAJ |
| description | Background/Aims: MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research has indicated that miR29b participates in DNA methylation by regulating DNA methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern and biological function of miR29b in mouse preimplantation embryonic development remain unknown. Methods: In this study, we examined the expression patterns of miR29b and Dnmt3a/3b in mouse early embryos at different developmental stages. Subsequently, expression and localization of DNMT3A/3B protein was analyzed in mouse early embryos by immunofluorescence staining. The biological function of miR29b in mouse early embryos was analyzed by microinjection of commercially available miRNA-specific inhibitors and mimics. Results: Our data showed that Dnmt3a/3b mRNA expression is negatively regulated by miR29b in mouse early embryos. Immunofluorescence analysis revealed that DNMT3A/3B protein expression is predominantly localized within the nucleoplasm of embryos. Alterations to the activity of miR29b could change the DNA methylation levels in mouse preimplantation embryos and lead to a developmental blockade, from the morula to the blastocyst stage. Conclusion: These results indicated a role for the miR29b-Dnmt3a/3b-DNA methylation axis in mouse early embryonic development, and we provide evidence that miR29b is indispensable for mouse early embryonic development. This study contributes to a preliminary understanding of the role of miR29b during mouse embryonic development. |
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| id | doaj.art-ee4faf4c437e44ed8af121f725ee7056 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-23T04:31:09Z |
| publishDate | 2015-02-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-ee4faf4c437e44ed8af121f725ee70562022-12-21T18:00:01ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-02-013531178118710.1159/000373942373942Expression and Potential Role of microRNA-29b in Mouse Early Embryo DevelopmentJunqiang ZhangYing WangXiaoguang LiuShenglin JiangChun ZhaoRong ShenXirong GuoXiufeng LingChang LiuBackground/Aims: MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research has indicated that miR29b participates in DNA methylation by regulating DNA methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern and biological function of miR29b in mouse preimplantation embryonic development remain unknown. Methods: In this study, we examined the expression patterns of miR29b and Dnmt3a/3b in mouse early embryos at different developmental stages. Subsequently, expression and localization of DNMT3A/3B protein was analyzed in mouse early embryos by immunofluorescence staining. The biological function of miR29b in mouse early embryos was analyzed by microinjection of commercially available miRNA-specific inhibitors and mimics. Results: Our data showed that Dnmt3a/3b mRNA expression is negatively regulated by miR29b in mouse early embryos. Immunofluorescence analysis revealed that DNMT3A/3B protein expression is predominantly localized within the nucleoplasm of embryos. Alterations to the activity of miR29b could change the DNA methylation levels in mouse preimplantation embryos and lead to a developmental blockade, from the morula to the blastocyst stage. Conclusion: These results indicated a role for the miR29b-Dnmt3a/3b-DNA methylation axis in mouse early embryonic development, and we provide evidence that miR29b is indispensable for mouse early embryonic development. This study contributes to a preliminary understanding of the role of miR29b during mouse embryonic development.http://www.karger.com/Article/FullText/373942MiR29bDnmt3a/3bEarly Embryonic DevelopmentDNA Methylation |
| spellingShingle | Junqiang Zhang Ying Wang Xiaoguang Liu Shenglin Jiang Chun Zhao Rong Shen Xirong Guo Xiufeng Ling Chang Liu Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development Cellular Physiology and Biochemistry MiR29b Dnmt3a/3b Early Embryonic Development DNA Methylation |
| title | Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development |
| title_full | Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development |
| title_fullStr | Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development |
| title_full_unstemmed | Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development |
| title_short | Expression and Potential Role of microRNA-29b in Mouse Early Embryo Development |
| title_sort | expression and potential role of microrna 29b in mouse early embryo development |
| topic | MiR29b Dnmt3a/3b Early Embryonic Development DNA Methylation |
| url | http://www.karger.com/Article/FullText/373942 |
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