Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment
Glioblastoma (GBM) is the most common and devastating primary brain tumor, leading to a uniform fatality after diagnosis. A major difficulty in eradicating GBM is the presence of microscopic residual infiltrating disease remaining after multimodality treatment. Glioma cancer stem cells (CSCs) have b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2020.558381/full |
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| author | Henry Ruiz-Garcia Henry Ruiz-Garcia Keila Alvarado-Estrada Paula Schiapparelli Alfredo Quinones-Hinojosa Daniel M. Trifiletti Daniel M. Trifiletti |
| author_facet | Henry Ruiz-Garcia Henry Ruiz-Garcia Keila Alvarado-Estrada Paula Schiapparelli Alfredo Quinones-Hinojosa Daniel M. Trifiletti Daniel M. Trifiletti |
| author_sort | Henry Ruiz-Garcia |
| collection | DOAJ |
| description | Glioblastoma (GBM) is the most common and devastating primary brain tumor, leading to a uniform fatality after diagnosis. A major difficulty in eradicating GBM is the presence of microscopic residual infiltrating disease remaining after multimodality treatment. Glioma cancer stem cells (CSCs) have been pinpointed as the treatment-resistant tumor component that seeds ultimate tumor progression. Despite the key role of CSCs, the ideal preclinical model to study the genetic and epigenetic landmarks driving their malignant behavior while simulating an accurate interaction with the tumor microenvironment (TME) is still missing. The introduction of three-dimensional (3D) tumor platforms, such as organoids and 3D bioprinting, has allowed for a better representation of the pathophysiologic interactions between glioma CSCs and the TME. Thus, these technologies have enabled a more detailed study of glioma biology, tumor angiogenesis, treatment resistance, and even performing high-throughput screening assays of drug susceptibility. First, we will review the foundation of glioma biology and biomechanics of the TME, and then the most up-to-date insights about the applicability of these new tools in malignant glioma research. |
| first_indexed | 2024-12-14T02:47:08Z |
| format | Article |
| id | doaj.art-ee534fba05924a8e8f6bd0e5b5fc5a3b |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-14T02:47:08Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-ee534fba05924a8e8f6bd0e5b5fc5a3b2022-12-21T23:19:51ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-10-011410.3389/fncel.2020.558381558381Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor MicroenvironmentHenry Ruiz-Garcia0Henry Ruiz-Garcia1Keila Alvarado-Estrada2Paula Schiapparelli3Alfredo Quinones-Hinojosa4Daniel M. Trifiletti5Daniel M. Trifiletti6Department of Radiation Oncology, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Radiation Oncology, Mayo Clinic, Jacksonville, FL, United StatesDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, United StatesGlioblastoma (GBM) is the most common and devastating primary brain tumor, leading to a uniform fatality after diagnosis. A major difficulty in eradicating GBM is the presence of microscopic residual infiltrating disease remaining after multimodality treatment. Glioma cancer stem cells (CSCs) have been pinpointed as the treatment-resistant tumor component that seeds ultimate tumor progression. Despite the key role of CSCs, the ideal preclinical model to study the genetic and epigenetic landmarks driving their malignant behavior while simulating an accurate interaction with the tumor microenvironment (TME) is still missing. The introduction of three-dimensional (3D) tumor platforms, such as organoids and 3D bioprinting, has allowed for a better representation of the pathophysiologic interactions between glioma CSCs and the TME. Thus, these technologies have enabled a more detailed study of glioma biology, tumor angiogenesis, treatment resistance, and even performing high-throughput screening assays of drug susceptibility. First, we will review the foundation of glioma biology and biomechanics of the TME, and then the most up-to-date insights about the applicability of these new tools in malignant glioma research.https://www.frontiersin.org/article/10.3389/fncel.2020.558381/fullgliomatumor microenvironmentstem cellbioprintingorganoidsorgan-on-a-chip |
| spellingShingle | Henry Ruiz-Garcia Henry Ruiz-Garcia Keila Alvarado-Estrada Paula Schiapparelli Alfredo Quinones-Hinojosa Daniel M. Trifiletti Daniel M. Trifiletti Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment Frontiers in Cellular Neuroscience glioma tumor microenvironment stem cell bioprinting organoids organ-on-a-chip |
| title | Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment |
| title_full | Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment |
| title_fullStr | Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment |
| title_full_unstemmed | Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment |
| title_short | Engineering Three-Dimensional Tumor Models to Study Glioma Cancer Stem Cells and Tumor Microenvironment |
| title_sort | engineering three dimensional tumor models to study glioma cancer stem cells and tumor microenvironment |
| topic | glioma tumor microenvironment stem cell bioprinting organoids organ-on-a-chip |
| url | https://www.frontiersin.org/article/10.3389/fncel.2020.558381/full |
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