Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach
The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin (BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the to...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20140008&year_id=2015&quarter_id=1&flag=1 |
| _version_ | 1811279509732720640 |
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| author | Zhong-Xiao Zhang Juan Su Xin-Ru Liu Wei-Xing Dai Shi-Kai Yan Wei-Dong Zhang |
| author_facet | Zhong-Xiao Zhang Juan Su Xin-Ru Liu Wei-Xing Dai Shi-Kai Yan Wei-Dong Zhang |
| author_sort | Zhong-Xiao Zhang |
| collection | DOAJ |
| description | The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin (BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administrated intraperitoneally (i.p.), and urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs. |
| first_indexed | 2024-04-13T00:56:53Z |
| format | Article |
| id | doaj.art-ee548cb54f2f4d0ea225d1f934e2021d |
| institution | Directory Open Access Journal |
| issn | 2311-8571 |
| language | English |
| last_indexed | 2024-04-13T00:56:53Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj.art-ee548cb54f2f4d0ea225d1f934e2021d2022-12-22T03:09:38ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712015-01-0111293610.15806/j.issn.2311-8571.2014.00082014.0008Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition ApproachZhong-Xiao Zhang0Juan Su1Xin-Ru Liu2Wei-Xing Dai3Shi-Kai Yan4Wei-Dong Zhang5School of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. ChinaSchool of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. ChinaSchool of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. ChinaSchool of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, P.R. ChinaSchool of Pharmacy, Second Military Medical University, Shanghai, 200433, P.R. ChinaThe purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin (BETX) using liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administrated intraperitoneally (i.p.), and urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs.http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20140008&year_id=2015&quarter_id=1&flag=1bupleurotoxinmetabolomic profilingtoxicitybiomarkerLC-Q-TOF-MS |
| spellingShingle | Zhong-Xiao Zhang Juan Su Xin-Ru Liu Wei-Xing Dai Shi-Kai Yan Wei-Dong Zhang Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach World Journal of Traditional Chinese Medicine bupleurotoxin metabolomic profiling toxicity biomarker LC-Q-TOF-MS |
| title | Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach |
| title_full | Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach |
| title_fullStr | Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach |
| title_full_unstemmed | Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach |
| title_short | Metabolomic Study of Biochemical Changes Related to Toxicity Induced by Bupleurotoxin Using LC-MS Coupled with a Pattern Recognition Approach |
| title_sort | metabolomic study of biochemical changes related to toxicity induced by bupleurotoxin using lc ms coupled with a pattern recognition approach |
| topic | bupleurotoxin metabolomic profiling toxicity biomarker LC-Q-TOF-MS |
| url | http://www.wjtcm.org:8080/ch/reader/create_pdf.aspx?file_no=20140008&year_id=2015&quarter_id=1&flag=1 |
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