Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia
PurposeOur study attempted to determine the prognostic significance of minimal residual disease (MRD) detected by a simplified flow cytometric assay during induction chemotherapy in children with B-cell acute lymphoblastic leukemia (B-ALL).MethodsA total of 98 patients were newly diagnosed with prec...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Pediatric Society
2010-11-01
|
| Series: | Korean Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://kjp.or.kr/upload/pdf/kjped-53-957.pdf |
| _version_ | 1818266000148135936 |
|---|---|
| author | Kyung Nam Koh Meerim Park Bo Eun Kim Ho Joon Im Chan-Jeoung Park Seongsoo Jang Hyun Sook Chi Jong Jin Seo |
| author_facet | Kyung Nam Koh Meerim Park Bo Eun Kim Ho Joon Im Chan-Jeoung Park Seongsoo Jang Hyun Sook Chi Jong Jin Seo |
| author_sort | Kyung Nam Koh |
| collection | DOAJ |
| description | PurposeOur study attempted to determine the prognostic significance of minimal residual disease (MRD) detected by a simplified flow cytometric assay during induction chemotherapy in children with B-cell acute lymphoblastic leukemia (B-ALL).MethodsA total of 98 patients were newly diagnosed with precursor B-ALL from June 2004 to December 2008 at the Asan Medical Center (Seoul, Korea). Of those, 37 were eligible for flow cytometric MRD study analysis on day 14 of their induction treatment. The flow cytometric MRD assay was based on the expression intensity of CD19/CD10/CD34 or aberrant expression of myeloid antigens by bone marrow nucleated cells.ResultsThirty-five patients (94.6%) had CD19-positive leukemic cells that also expressed CD10 and/or CD34, and 18 (48.6%) had leukemic cells with aberrant expression of myeloid antigens. Seven patients with ≥1% leukemic cells on day 14 had a significantly lower relapse-free survival (RFS) compared to the 30 patients with lower levels (42.9% [18.7%] vs. 92.0% [5.4%], P=0.004). Stratification into 3 MRD groups (≥1%, 0.1-1%, and <0.1%) also showed a statistically significant difference in RFS (42.9% [18.7%] vs. 86.9% [8.7%] vs. 100%, P=0.013). However, the MRD status had no significant influence on overall survival. Multivariate analysis demonstrated that the MRD level on day 14 was an independent prognostic factor with borderline significance.ConclusionAn MRD assay using simplified flow cytometry during induction chemotherapy may help to identify patients with B-ALL who have an excellent outcome and patients who are at higher risk for relapse. |
| first_indexed | 2024-12-12T19:59:44Z |
| format | Article |
| id | doaj.art-ee57450ce4054a738a87638c3566a793 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-12-12T19:59:44Z |
| publishDate | 2010-11-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-ee57450ce4054a738a87638c3566a7932022-12-22T00:13:47ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582010-11-01531195796410.3345/kjp.2010.53.11.9572010531106Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemiaKyung Nam Koh0Meerim Park1Bo Eun Kim2Ho Joon Im3Chan-Jeoung Park4Seongsoo Jang5Hyun Sook Chi6Jong Jin Seo7Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.PurposeOur study attempted to determine the prognostic significance of minimal residual disease (MRD) detected by a simplified flow cytometric assay during induction chemotherapy in children with B-cell acute lymphoblastic leukemia (B-ALL).MethodsA total of 98 patients were newly diagnosed with precursor B-ALL from June 2004 to December 2008 at the Asan Medical Center (Seoul, Korea). Of those, 37 were eligible for flow cytometric MRD study analysis on day 14 of their induction treatment. The flow cytometric MRD assay was based on the expression intensity of CD19/CD10/CD34 or aberrant expression of myeloid antigens by bone marrow nucleated cells.ResultsThirty-five patients (94.6%) had CD19-positive leukemic cells that also expressed CD10 and/or CD34, and 18 (48.6%) had leukemic cells with aberrant expression of myeloid antigens. Seven patients with ≥1% leukemic cells on day 14 had a significantly lower relapse-free survival (RFS) compared to the 30 patients with lower levels (42.9% [18.7%] vs. 92.0% [5.4%], P=0.004). Stratification into 3 MRD groups (≥1%, 0.1-1%, and <0.1%) also showed a statistically significant difference in RFS (42.9% [18.7%] vs. 86.9% [8.7%] vs. 100%, P=0.013). However, the MRD status had no significant influence on overall survival. Multivariate analysis demonstrated that the MRD level on day 14 was an independent prognostic factor with borderline significance.ConclusionAn MRD assay using simplified flow cytometry during induction chemotherapy may help to identify patients with B-ALL who have an excellent outcome and patients who are at higher risk for relapse.http://kjp.or.kr/upload/pdf/kjped-53-957.pdfLymphoblastic leukemiaAcuteChildhoodMinimal residual diseaseFlow cytometry |
| spellingShingle | Kyung Nam Koh Meerim Park Bo Eun Kim Ho Joon Im Chan-Jeoung Park Seongsoo Jang Hyun Sook Chi Jong Jin Seo Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia Korean Journal of Pediatrics Lymphoblastic leukemia Acute Childhood Minimal residual disease Flow cytometry |
| title | Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| title_full | Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| title_fullStr | Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| title_full_unstemmed | Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| title_short | Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| title_sort | prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia |
| topic | Lymphoblastic leukemia Acute Childhood Minimal residual disease Flow cytometry |
| url | http://kjp.or.kr/upload/pdf/kjped-53-957.pdf |
| work_keys_str_mv | AT kyungnamkoh prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT meerimpark prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT boeunkim prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT hojoonim prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT chanjeoungpark prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT seongsoojang prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT hyunsookchi prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia AT jongjinseo prognosticsignificanceofminimalresidualdiseasedetectedbyasimplifiedflowcytometricassayduringremissioninductionchemotherapyinchildrenwithacutelymphoblasticleukemia |