A UHPLC METHOD FOR SERTRALINE DETERMINATION
Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples – mostly human blood and urine. Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxicati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Peytchinski Publishing
2017-11-01
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| Series: | Journal of IMAB |
| Subjects: | |
| Online Access: | http://www.journal-imab-bg.org/issues-2017/issue4/JofIMAB-2017-23-4p1765-1768.pdf |
| _version_ | 1818051255052795904 |
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| author | Georgi Bonchev Snezha Zlateva Petko Marinov Marieta Yovcheva Ivaylo Vazharov |
| author_facet | Georgi Bonchev Snezha Zlateva Petko Marinov Marieta Yovcheva Ivaylo Vazharov |
| author_sort | Georgi Bonchev |
| collection | DOAJ |
| description | Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples – mostly human blood and urine.
Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxications and toxicoallergies were used during method validation and case monitoring. Analytical identification of sertraline and/or metabolites was done by GC-MS. Gas chromatography coupled with flame ionization detection was used for alcohols/volatiles screening of clinical samples. Ultra high-performance liquid chromatography system in tandem with diode-array detector has been used as the main quantitative instrument.
Results: After critical consideration of available reference data a carefully set of experimental conditions for sertraline extraction and UHPLC determination were adopted and optimized. Preliminary liquid-phase sample purification was applied. Zorbax Extend-C18 column (150 x 4.6 mm, 5 µm) was used under isocratic conditions with phosphate buffer (pH 2.7; 10 mM) containing 1.5 ml L–1 triethylamine – acetonitrile (65:35, v/v) at 20oC, at the flow-rate of 1.0 mL/min and UV detection at 220 nm. This method was validated for the determination of sertraline in human plasma/serum samples (70% recovery).
Conclusions: A simple yet sensitive and reliable method for sertraline determination was introduced. Linearity over 20-1000 ng mL–1 range was shown; LOQ was 20 ng mL–1. The method was clinically applied for monitoring the blood sertraline levels during a course of detoxication of a female patient. |
| first_indexed | 2024-12-10T11:06:27Z |
| format | Article |
| id | doaj.art-ee57892e355b4f93b52afa8002c48649 |
| institution | Directory Open Access Journal |
| issn | 1312-773X |
| language | English |
| last_indexed | 2024-12-10T11:06:27Z |
| publishDate | 2017-11-01 |
| publisher | Peytchinski Publishing |
| record_format | Article |
| series | Journal of IMAB |
| spelling | doaj.art-ee57892e355b4f93b52afa8002c486492022-12-22T01:51:33ZengPeytchinski PublishingJournal of IMAB1312-773X2017-11-012341765176810.5272/jimab.2017234.1765A UHPLC METHOD FOR SERTRALINE DETERMINATIONGeorgi Bonchev0Snezha Zlateva1Petko Marinov2Marieta Yovcheva3Ivaylo Vazharov4Clinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, BulgariaClinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, BulgariaClinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, BulgariaClinic for Intensive Treatment of Acute Intoxications and Toxicoallergies, Naval Hospital – Varna, Military Medical Academy, BulgariaClinic of Internal Diseases, Naval Hospital – Varna, Military Medical Academy, Bulgaria.Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples – mostly human blood and urine. Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxications and toxicoallergies were used during method validation and case monitoring. Analytical identification of sertraline and/or metabolites was done by GC-MS. Gas chromatography coupled with flame ionization detection was used for alcohols/volatiles screening of clinical samples. Ultra high-performance liquid chromatography system in tandem with diode-array detector has been used as the main quantitative instrument. Results: After critical consideration of available reference data a carefully set of experimental conditions for sertraline extraction and UHPLC determination were adopted and optimized. Preliminary liquid-phase sample purification was applied. Zorbax Extend-C18 column (150 x 4.6 mm, 5 µm) was used under isocratic conditions with phosphate buffer (pH 2.7; 10 mM) containing 1.5 ml L–1 triethylamine – acetonitrile (65:35, v/v) at 20oC, at the flow-rate of 1.0 mL/min and UV detection at 220 nm. This method was validated for the determination of sertraline in human plasma/serum samples (70% recovery). Conclusions: A simple yet sensitive and reliable method for sertraline determination was introduced. Linearity over 20-1000 ng mL–1 range was shown; LOQ was 20 ng mL–1. The method was clinically applied for monitoring the blood sertraline levels during a course of detoxication of a female patient.http://www.journal-imab-bg.org/issues-2017/issue4/JofIMAB-2017-23-4p1765-1768.pdfsertralineZoloftHPLC determinationdrug monitoring |
| spellingShingle | Georgi Bonchev Snezha Zlateva Petko Marinov Marieta Yovcheva Ivaylo Vazharov A UHPLC METHOD FOR SERTRALINE DETERMINATION Journal of IMAB sertraline Zoloft HPLC determination drug monitoring |
| title | A UHPLC METHOD FOR SERTRALINE DETERMINATION |
| title_full | A UHPLC METHOD FOR SERTRALINE DETERMINATION |
| title_fullStr | A UHPLC METHOD FOR SERTRALINE DETERMINATION |
| title_full_unstemmed | A UHPLC METHOD FOR SERTRALINE DETERMINATION |
| title_short | A UHPLC METHOD FOR SERTRALINE DETERMINATION |
| title_sort | uhplc method for sertraline determination |
| topic | sertraline Zoloft HPLC determination drug monitoring |
| url | http://www.journal-imab-bg.org/issues-2017/issue4/JofIMAB-2017-23-4p1765-1768.pdf |
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