Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus
The ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. In Bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-10-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/13/10/6472 |
| _version_ | 1818013429639675904 |
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| author | Blanda Di Luccia Antonio Riccio Adele Vanacore Loredana Baccigalupi Antonio Molinaro Ezio Ricca |
| author_facet | Blanda Di Luccia Antonio Riccio Adele Vanacore Loredana Baccigalupi Antonio Molinaro Ezio Ricca |
| author_sort | Blanda Di Luccia |
| collection | DOAJ |
| description | The ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. In Bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetative growth is no longer feasible. While in B. subtilis the genetic system controlling matrix production has been studied in detail, it is still unclear whether other spore formers utilize similar mechanisms. We report that SF214, a pigmented strain of Bacillus pumilus isolated from the marine environment, can produce an extracellular matrix relying on orthologs of many of the genes known to be important for matrix synthesis in B. subtilis. We also report a characterization of the carbohydrates forming the extracellular matrix of strain SF214. The isolation and characterization of mutants altered in matrix synthesis, pigmentation, and spore formation suggest that in strain SF214 the three processes are strictly interconnected and regulated by a common molecular mechanism. |
| first_indexed | 2024-04-14T06:33:03Z |
| format | Article |
| id | doaj.art-ee5987b93e8a4db9b9f9acf5ab977fce |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T06:33:03Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-ee5987b93e8a4db9b9f9acf5ab977fce2022-12-22T02:07:33ZengMDPI AGMarine Drugs1660-33972015-10-0113106472648810.3390/md13106472md13106472Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilusBlanda Di Luccia0Antonio Riccio1Adele Vanacore2Loredana Baccigalupi3Antonio Molinaro4Ezio Ricca5Department of Biology, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyDepartment of Biology, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyDepartment of Chemistry, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyDepartment of Biology, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyDepartment of Chemistry, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyDepartment of Biology, Federico II University, MSA, via Cinthia 21, 80126 Naples, ItalyThe ability to produce an extracellular matrix and form multicellular communities is an adaptive behavior shared by many bacteria. In Bacillus subtilis, the model system for spore-forming bacteria, matrix production is one of the possible differentiation pathways that a cell can follow when vegetative growth is no longer feasible. While in B. subtilis the genetic system controlling matrix production has been studied in detail, it is still unclear whether other spore formers utilize similar mechanisms. We report that SF214, a pigmented strain of Bacillus pumilus isolated from the marine environment, can produce an extracellular matrix relying on orthologs of many of the genes known to be important for matrix synthesis in B. subtilis. We also report a characterization of the carbohydrates forming the extracellular matrix of strain SF214. The isolation and characterization of mutants altered in matrix synthesis, pigmentation, and spore formation suggest that in strain SF214 the three processes are strictly interconnected and regulated by a common molecular mechanism.http://www.mdpi.com/1660-3397/13/10/6472SF214microbial differentiationexopolysaccharide |
| spellingShingle | Blanda Di Luccia Antonio Riccio Adele Vanacore Loredana Baccigalupi Antonio Molinaro Ezio Ricca Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus Marine Drugs SF214 microbial differentiation exopolysaccharide |
| title | Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus |
| title_full | Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus |
| title_fullStr | Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus |
| title_full_unstemmed | Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus |
| title_short | Matrix Production, Pigment Synthesis, and Sporulation in a Marine Isolated Strain of Bacillus pumilus |
| title_sort | matrix production pigment synthesis and sporulation in a marine isolated strain of bacillus pumilus |
| topic | SF214 microbial differentiation exopolysaccharide |
| url | http://www.mdpi.com/1660-3397/13/10/6472 |
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