Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland
<i>Klebsiella pneumoniae</i> is one of the most common etiological agents isolated from epidemic outbreaks in neonatal wards. We describe how an extended-spectrum β-lactamase-producing <i>K. pneumoniae</i> (ESBL-KP) outbreak in a neonatal ward was extinguished. During the out...
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MDPI AG
2022-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/1/50 |
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| author | Agata Pruss Paweł Kwiatkowski Helena Masiuk Iwona Bilska Stefania Giedrys-Kalemba Barbara Dołęgowska |
| author_facet | Agata Pruss Paweł Kwiatkowski Helena Masiuk Iwona Bilska Stefania Giedrys-Kalemba Barbara Dołęgowska |
| author_sort | Agata Pruss |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> is one of the most common etiological agents isolated from epidemic outbreaks in neonatal wards. We describe how an extended-spectrum β-lactamase-producing <i>K. pneumoniae</i> (ESBL-KP) outbreak in a neonatal ward was extinguished. During the outbreak, which lasted over two months, 26 neonates were tested for <i>K. pneumoniae</i>, and 42 environmental swabs were taken. Drug susceptibility was determined for the isolated strains, and their virulence and phylogenetic similarity were checked. ESBL-KP colonization was confirmed in 18 neonates, and six were also confirmed to be infected. All strains isolated from patients represented one clonal type, <i>K. pneumoniae.</i> One strain isolated from an environmental source was determined to be a unique pulsed-field gel electrophoresis pattern. Gestational age and Apgar score were assessed as statistically significant for neonates with ESBL-KP infection. The epidemiological measures taken have been successful, and no further cases appeared. Immediate tightening of hospital hygiene rules, screening of all hospitalized neonates, and cohorting ESBL-KP-positive patients proved effective in controlling and ending the outbreak. The lack of ESBL-KP in the environment suggests that the outbreak was transmitted by colonized hospital staff. This theory could be confirmed by introducing mandatory screening for medical personnel. |
| first_indexed | 2024-03-09T13:48:38Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T13:48:38Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj.art-ee5bae9d9a58428fb92a1f5b5ac344442023-11-30T20:55:00ZengMDPI AGAntibiotics2079-63822022-12-011215010.3390/antibiotics12010050Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in PolandAgata Pruss0Paweł Kwiatkowski1Helena Masiuk2Iwona Bilska3Stefania Giedrys-Kalemba4Barbara Dołęgowska5Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Medical Microbiology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandMicrobiological Laboratory, Independent Public Clinical Hospital No. 1 in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, PolandDepartment of Medical Microbiology, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland<i>Klebsiella pneumoniae</i> is one of the most common etiological agents isolated from epidemic outbreaks in neonatal wards. We describe how an extended-spectrum β-lactamase-producing <i>K. pneumoniae</i> (ESBL-KP) outbreak in a neonatal ward was extinguished. During the outbreak, which lasted over two months, 26 neonates were tested for <i>K. pneumoniae</i>, and 42 environmental swabs were taken. Drug susceptibility was determined for the isolated strains, and their virulence and phylogenetic similarity were checked. ESBL-KP colonization was confirmed in 18 neonates, and six were also confirmed to be infected. All strains isolated from patients represented one clonal type, <i>K. pneumoniae.</i> One strain isolated from an environmental source was determined to be a unique pulsed-field gel electrophoresis pattern. Gestational age and Apgar score were assessed as statistically significant for neonates with ESBL-KP infection. The epidemiological measures taken have been successful, and no further cases appeared. Immediate tightening of hospital hygiene rules, screening of all hospitalized neonates, and cohorting ESBL-KP-positive patients proved effective in controlling and ending the outbreak. The lack of ESBL-KP in the environment suggests that the outbreak was transmitted by colonized hospital staff. This theory could be confirmed by introducing mandatory screening for medical personnel.https://www.mdpi.com/2079-6382/12/1/50neonatesoutbreakneonatal clinicextended-spectrum β-lactamase-producing <i>Klebsiella pneumoniae</i> |
| spellingShingle | Agata Pruss Paweł Kwiatkowski Helena Masiuk Iwona Bilska Stefania Giedrys-Kalemba Barbara Dołęgowska Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland Antibiotics neonates outbreak neonatal clinic extended-spectrum β-lactamase-producing <i>Klebsiella pneumoniae</i> |
| title | Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland |
| title_full | Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland |
| title_fullStr | Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland |
| title_full_unstemmed | Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland |
| title_short | Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> Outbreak in a Neonatal Clinic in Poland |
| title_sort | epidemiological analysis of extended spectrum β lactamase producing i klebsiella pneumoniae i outbreak in a neonatal clinic in poland |
| topic | neonates outbreak neonatal clinic extended-spectrum β-lactamase-producing <i>Klebsiella pneumoniae</i> |
| url | https://www.mdpi.com/2079-6382/12/1/50 |
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