Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA
In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C1...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2014-12-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859&lng=en&tlng=en |
| _version_ | 1819024751660630016 |
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| author | Caroline Danziato Rodrigues Najeh Maissar Khalil Rubiana Mara Mainardes |
| author_facet | Caroline Danziato Rodrigues Najeh Maissar Khalil Rubiana Mara Mainardes |
| author_sort | Caroline Danziato Rodrigues |
| collection | DOAJ |
| description | In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The LOQ and LOD were 55 and 18 ng/mL, respectively. The mean recovery of AmB from the samples was 99.92% (relative standard deviation (RSD) = 0.34%, n=9), and the method was robust for changes in the flow rate of the mobile phase (maximum RSD=4.82%). The intra- and inter-assay coefficients of variation were less than 0.59%. The method was successfully used to determine the entrapment efficiency of AmB in PLA-PEG blend nanoparticles. |
| first_indexed | 2024-12-21T04:59:46Z |
| format | Article |
| id | doaj.art-ee5caafd7b194a3db71ebc1d96dac6ca |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-21T04:59:46Z |
| publishDate | 2014-12-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-ee5caafd7b194a3db71ebc1d96dac6ca2022-12-21T19:15:16ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902014-12-0150485986810.1590/S1984-82502014000400021S1984-82502014000400859Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDACaroline Danziato RodriguesNajeh Maissar KhalilRubiana Mara MainardesIn this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The LOQ and LOD were 55 and 18 ng/mL, respectively. The mean recovery of AmB from the samples was 99.92% (relative standard deviation (RSD) = 0.34%, n=9), and the method was robust for changes in the flow rate of the mobile phase (maximum RSD=4.82%). The intra- and inter-assay coefficients of variation were less than 0.59%. The method was successfully used to determine the entrapment efficiency of AmB in PLA-PEG blend nanoparticles.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859&lng=en&tlng=enAnfotericina B/determinaçãoAnfotericina B/encapsulação em nanopartículasCromatografia líquida de alta eficiência/fase reversaNanopartículas |
| spellingShingle | Caroline Danziato Rodrigues Najeh Maissar Khalil Rubiana Mara Mainardes Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA Brazilian Journal of Pharmaceutical Sciences Anfotericina B/determinação Anfotericina B/encapsulação em nanopartículas Cromatografia líquida de alta eficiência/fase reversa Nanopartículas |
| title | Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA |
| title_full | Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA |
| title_fullStr | Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA |
| title_full_unstemmed | Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA |
| title_short | Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA |
| title_sort | determination of amphotericin b in pla peg blend nanoparticles by hplc pda |
| topic | Anfotericina B/determinação Anfotericina B/encapsulação em nanopartículas Cromatografia líquida de alta eficiência/fase reversa Nanopartículas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859&lng=en&tlng=en |
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