Embryonic diapause is conserved across mammals.
Embryonic diapause (ED) is a temporary arrest of embryo development and is characterized by delayed implantation in the uterus. ED occurs in blastocysts of less than 2% of mammalian species, including the mouse (Mus musculus). If ED were an evolutionarily conserved phenomenon, then it should be indu...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3299720?pdf=render |
| _version_ | 1811234613287190528 |
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| author | Grazyna E Ptak Emanuela Tacconi Marta Czernik Paola Toschi Jacek A Modlinski Pasqualino Loi |
| author_facet | Grazyna E Ptak Emanuela Tacconi Marta Czernik Paola Toschi Jacek A Modlinski Pasqualino Loi |
| author_sort | Grazyna E Ptak |
| collection | DOAJ |
| description | Embryonic diapause (ED) is a temporary arrest of embryo development and is characterized by delayed implantation in the uterus. ED occurs in blastocysts of less than 2% of mammalian species, including the mouse (Mus musculus). If ED were an evolutionarily conserved phenomenon, then it should be inducible in blastocysts of normally non-diapausing mammals, such as domestic species. To prove this hypothesis, we examined whether blastocysts from domestic sheep (Ovis aries) could enter into diapause following their transfer into mouse uteri in which diapause conditions were induced. Sheep blastocysts entered into diapause, as demonstrated by growth arrest, viability maintenance and their ED-specific pattern of gene expression. Seven days after transfer, diapausing ovine blastocysts were able to resume growth in vitro and, after transfer to surrogate ewe recipients, to develop into normal lambs. The finding that non-diapausing ovine embryos can enter into diapause implies that this phenomenon is phylogenetically conserved and not secondarily acquired by embryos of diapausing species. Our study questions the current model of independent evolution of ED in different mammalian orders. |
| first_indexed | 2024-04-12T11:39:09Z |
| format | Article |
| id | doaj.art-ee5cd9a32a0041f08cce4f7abb01dbd4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:39:09Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ee5cd9a32a0041f08cce4f7abb01dbd42022-12-22T03:34:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3302710.1371/journal.pone.0033027Embryonic diapause is conserved across mammals.Grazyna E PtakEmanuela TacconiMarta CzernikPaola ToschiJacek A ModlinskiPasqualino LoiEmbryonic diapause (ED) is a temporary arrest of embryo development and is characterized by delayed implantation in the uterus. ED occurs in blastocysts of less than 2% of mammalian species, including the mouse (Mus musculus). If ED were an evolutionarily conserved phenomenon, then it should be inducible in blastocysts of normally non-diapausing mammals, such as domestic species. To prove this hypothesis, we examined whether blastocysts from domestic sheep (Ovis aries) could enter into diapause following their transfer into mouse uteri in which diapause conditions were induced. Sheep blastocysts entered into diapause, as demonstrated by growth arrest, viability maintenance and their ED-specific pattern of gene expression. Seven days after transfer, diapausing ovine blastocysts were able to resume growth in vitro and, after transfer to surrogate ewe recipients, to develop into normal lambs. The finding that non-diapausing ovine embryos can enter into diapause implies that this phenomenon is phylogenetically conserved and not secondarily acquired by embryos of diapausing species. Our study questions the current model of independent evolution of ED in different mammalian orders.http://europepmc.org/articles/PMC3299720?pdf=render |
| spellingShingle | Grazyna E Ptak Emanuela Tacconi Marta Czernik Paola Toschi Jacek A Modlinski Pasqualino Loi Embryonic diapause is conserved across mammals. PLoS ONE |
| title | Embryonic diapause is conserved across mammals. |
| title_full | Embryonic diapause is conserved across mammals. |
| title_fullStr | Embryonic diapause is conserved across mammals. |
| title_full_unstemmed | Embryonic diapause is conserved across mammals. |
| title_short | Embryonic diapause is conserved across mammals. |
| title_sort | embryonic diapause is conserved across mammals |
| url | http://europepmc.org/articles/PMC3299720?pdf=render |
| work_keys_str_mv | AT grazynaeptak embryonicdiapauseisconservedacrossmammals AT emanuelatacconi embryonicdiapauseisconservedacrossmammals AT martaczernik embryonicdiapauseisconservedacrossmammals AT paolatoschi embryonicdiapauseisconservedacrossmammals AT jacekamodlinski embryonicdiapauseisconservedacrossmammals AT pasqualinoloi embryonicdiapauseisconservedacrossmammals |