Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i>
Plant senescence is a complex process that is controlled by developmental regulation and genetic programs. A senescence-related gene <i>CpSRG1</i>, which belongs to the 2OG-Fe(II) dioxygenase superfamily, was characterized from wintersweet, and the phylogenetic relationship of CpSRG1 wit...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13971 |
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| author | Yinzhu Cao Guixiang Li Xia Wang Renwei Huang Jianghui Luo Mingyang Li Daofeng Liu Shunzhao Sui |
| author_facet | Yinzhu Cao Guixiang Li Xia Wang Renwei Huang Jianghui Luo Mingyang Li Daofeng Liu Shunzhao Sui |
| author_sort | Yinzhu Cao |
| collection | DOAJ |
| description | Plant senescence is a complex process that is controlled by developmental regulation and genetic programs. A senescence-related gene <i>CpSRG1</i>, which belongs to the 2OG-Fe(II) dioxygenase superfamily, was characterized from wintersweet, and the phylogenetic relationship of CpSRG1 with homologs from other species was investigated. The expression analysis by qRT-PCR (quantitative real-time PCR) indicated that <i>CpSRG1</i> is abundant in flower organs, especially in petals and stamens, and the highest expression of <i>CpSRG1</i> was detected in stage 6 (withering period). The expression patterns of the <i>CpSRG1</i> gene were further confirmed in <i>CpSRG1pro::GUS</i> (<i>β-glucuronidase</i>) plants, and the activity of the <i>CpSRG1</i> promoter was enhanced by exogenous Eth (ethylene), SA (salicylic acid), and GA<sub>3</sub> (gibberellin). Heterologous overexpression of <i>CpSRG1</i> in <i>Arabidopsis</i> promoted growth and flowering, and delayed senescence. Moreover, the survival rates were significantly higher and the root lengths were significantly longer in the transgenic lines than in the wild-type plants, both under low nitrogen stress and GA<sub>3</sub> treatment. This indicated that the <i>CpSRG1</i> gene may promote the synthesis of assimilates in plants through the GA pathway, thereby improving growth and flowering, and delaying senescence in transgenic <i>Arabidopsis</i>. Our study has laid a satisfactory foundation for further analysis of senescence-related genes in wintersweet and wood plants. It also enriched our knowledge of the 2OG-Fe(II) dioxygenase superfamily, which plays a variety of important roles in plants. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ee5dd6858483407c89e9a5efd9ff01e12023-11-24T08:36:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221397110.3390/ijms232213971Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i>Yinzhu Cao0Guixiang Li1Xia Wang2Renwei Huang3Jianghui Luo4Mingyang Li5Daofeng Liu6Shunzhao Sui7Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaPlant senescence is a complex process that is controlled by developmental regulation and genetic programs. A senescence-related gene <i>CpSRG1</i>, which belongs to the 2OG-Fe(II) dioxygenase superfamily, was characterized from wintersweet, and the phylogenetic relationship of CpSRG1 with homologs from other species was investigated. The expression analysis by qRT-PCR (quantitative real-time PCR) indicated that <i>CpSRG1</i> is abundant in flower organs, especially in petals and stamens, and the highest expression of <i>CpSRG1</i> was detected in stage 6 (withering period). The expression patterns of the <i>CpSRG1</i> gene were further confirmed in <i>CpSRG1pro::GUS</i> (<i>β-glucuronidase</i>) plants, and the activity of the <i>CpSRG1</i> promoter was enhanced by exogenous Eth (ethylene), SA (salicylic acid), and GA<sub>3</sub> (gibberellin). Heterologous overexpression of <i>CpSRG1</i> in <i>Arabidopsis</i> promoted growth and flowering, and delayed senescence. Moreover, the survival rates were significantly higher and the root lengths were significantly longer in the transgenic lines than in the wild-type plants, both under low nitrogen stress and GA<sub>3</sub> treatment. This indicated that the <i>CpSRG1</i> gene may promote the synthesis of assimilates in plants through the GA pathway, thereby improving growth and flowering, and delaying senescence in transgenic <i>Arabidopsis</i>. Our study has laid a satisfactory foundation for further analysis of senescence-related genes in wintersweet and wood plants. It also enriched our knowledge of the 2OG-Fe(II) dioxygenase superfamily, which plays a variety of important roles in plants.https://www.mdpi.com/1422-0067/23/22/13971promoterthe 2OG-Fe(II) dioxygenase superfamilytransgenic <i>Arabidopsis</i>senescencewintersweet |
| spellingShingle | Yinzhu Cao Guixiang Li Xia Wang Renwei Huang Jianghui Luo Mingyang Li Daofeng Liu Shunzhao Sui Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> International Journal of Molecular Sciences promoter the 2OG-Fe(II) dioxygenase superfamily transgenic <i>Arabidopsis</i> senescence wintersweet |
| title | Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> |
| title_full | Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> |
| title_fullStr | Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> |
| title_short | Overexpression of a Senescence-Related Gene <i>CpSRG1</i> from Wintersweet (<i>Chimonanthus praecox</i>) Promoted Growth and Flowering, and Delayed Senescence in Transgenic <i>Arabidopsis</i> |
| title_sort | overexpression of a senescence related gene i cpsrg1 i from wintersweet i chimonanthus praecox i promoted growth and flowering and delayed senescence in transgenic i arabidopsis i |
| topic | promoter the 2OG-Fe(II) dioxygenase superfamily transgenic <i>Arabidopsis</i> senescence wintersweet |
| url | https://www.mdpi.com/1422-0067/23/22/13971 |
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