Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control
IntroductionAphids form a stable and mutually beneficial relationship with their primary symbiont Buchnera aphidicola, which play an important role in providing the missing nutrients to the host aphid. Based on the genome sequence of wheat aphid Siotobion miscanthi and its primary symbiont Buchnera...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1092638/full |
| _version_ | 1797950588277751808 |
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| author | Qian Li Qian Li Yu Cheng Jia Fan Julian Chen Julian Chen |
| author_facet | Qian Li Qian Li Yu Cheng Jia Fan Julian Chen Julian Chen |
| author_sort | Qian Li |
| collection | DOAJ |
| description | IntroductionAphids form a stable and mutually beneficial relationship with their primary symbiont Buchnera aphidicola, which play an important role in providing the missing nutrients to the host aphid. Based on the genome sequence of wheat aphid Siotobion miscanthi and its primary symbiont Buchnera that we obtained in our previously study, we identified a metabolic relay gene, ilvA, involved in the isoleucine synthesis pathway between aphids and Buchnera.MethodIn this study, we identified the location and sequence structure of ilvA gene in aphid genome, the expression level in different instars and tissues of aphids, and the effect of reducing ilvA expression on the growth and development of aphids by bioinformatics analysis, quantitative PCR, RNAi and bioassay experiments.ResultOur study showed that ilvA was expressed at the highest level in the 2nd instar of the aphid, while the expression of this gene was significantly higher in the aphid bacteriocytes than in other tissues. Notably, this gene is localized on the aphid sex chromosome and remains highly conserved and collinearity across different aphid genomes. Knocking down the expression of ilvA reduced the aphid body weight and production. However, the indices of mortality decreased slightly, but were not significantly different, compared to the control.DiscussionThe results show that the relay genes between aphids and their symbionts in the metabolism of essential nutrients have potential roles in the growth and development of aphids, meanwhile, providing target loci and new ideas for RNAi-based aphid green control strategies. |
| first_indexed | 2024-04-10T22:18:07Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T22:18:07Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ee614ac6f7c54b0887645e2a99cfe98a2023-01-18T07:19:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10926381092638Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid controlQian Li0Qian Li1Yu Cheng2Jia Fan3Julian Chen4Julian Chen5College of Bioscience and Resource Environment/Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Beijing University of Agriculture, Beijing, ChinaThe State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaMinistry of Agricultural and Rural Affairs -Center of Applied Biological International (MARA-CABI) Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaIntroductionAphids form a stable and mutually beneficial relationship with their primary symbiont Buchnera aphidicola, which play an important role in providing the missing nutrients to the host aphid. Based on the genome sequence of wheat aphid Siotobion miscanthi and its primary symbiont Buchnera that we obtained in our previously study, we identified a metabolic relay gene, ilvA, involved in the isoleucine synthesis pathway between aphids and Buchnera.MethodIn this study, we identified the location and sequence structure of ilvA gene in aphid genome, the expression level in different instars and tissues of aphids, and the effect of reducing ilvA expression on the growth and development of aphids by bioinformatics analysis, quantitative PCR, RNAi and bioassay experiments.ResultOur study showed that ilvA was expressed at the highest level in the 2nd instar of the aphid, while the expression of this gene was significantly higher in the aphid bacteriocytes than in other tissues. Notably, this gene is localized on the aphid sex chromosome and remains highly conserved and collinearity across different aphid genomes. Knocking down the expression of ilvA reduced the aphid body weight and production. However, the indices of mortality decreased slightly, but were not significantly different, compared to the control.DiscussionThe results show that the relay genes between aphids and their symbionts in the metabolism of essential nutrients have potential roles in the growth and development of aphids, meanwhile, providing target loci and new ideas for RNAi-based aphid green control strategies.https://www.frontiersin.org/articles/10.3389/fpls.2022.1092638/fullSitobin miscanthiBuchnera aphidicolailvAmetabolic relayRNAi |
| spellingShingle | Qian Li Qian Li Yu Cheng Jia Fan Julian Chen Julian Chen Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control Frontiers in Plant Science Sitobin miscanthi Buchnera aphidicola ilvA metabolic relay RNAi |
| title | Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control |
| title_full | Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control |
| title_fullStr | Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control |
| title_full_unstemmed | Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control |
| title_short | Metabolic relay gene of aphid and primary symbiont as RNAi target loci for aphid control |
| title_sort | metabolic relay gene of aphid and primary symbiont as rnai target loci for aphid control |
| topic | Sitobin miscanthi Buchnera aphidicola ilvA metabolic relay RNAi |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1092638/full |
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