Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors
Objective To investigate the clinical utility of identifying circulating tumor cells (CTC) and circulating tumorigenic endothelial cells (CTEC) in skin malignancies. Methods Subtraction enrichment-immunostaining and fluorescence in situ hybridization (SE-iFISH) were used to detect the number of CTC...
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2023-06-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
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| Online Access: | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.03.003 |
| _version_ | 1797374058525884416 |
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| author | Guiyue CAI Sirui LI Ying LIU Rongyi CHEN Sujun LUO Ping LIN |
| author_facet | Guiyue CAI Sirui LI Ying LIU Rongyi CHEN Sujun LUO Ping LIN |
| author_sort | Guiyue CAI |
| collection | DOAJ |
| description | Objective To investigate the clinical utility of identifying circulating tumor cells (CTC) and circulating tumorigenic endothelial cells (CTEC) in skin malignancies. Methods Subtraction enrichment-immunostaining and fluorescence in situ hybridization (SE-iFISH) were used to detect the number of CTC and CTEC in the peripheral blood of 5 patients with skin tumor (tumor group) and 14 healthy individuals (healthy controls), and the detection rate was calculated. The subtype characteristics of CTC/CTEC and the correlation between the number of CTC/CTEC and the clinical characteristics of the patients were analyzed. Results The positive rates of CTC and CTEC in the tumor group were 100%. The average CTC were (6.93±8.18) /mL and CTEC were (1.60±1.03) /mL in the peripheral blood of the tumor group. The positive rates of CTC and CTEC in the healthy controls were both 64%. The average CTC were (0.19±0.21) /mL, and CTEC were (0.30±0.33) /mL in the peripheral blood of the healthy controls. Patients with larger diameter of tumor, lymph node metastasis, and clinical stages of III and IV had higher levels of CTC and CTEC; Large CTC accounted for 88.46% (184/208) of the total tumor cells, while large CTEC accounted for 81.25% (39/48) of the total tumor cells, which mainly were ≥ pentaploid CTC and CTEC. Conclusions Using SE-iFISH, the detection rates of CTC/CTEC are high in patients with skin tumors. The amounts of CTC and CTEC are correlated with the clinical features of patients. A higher proportion of CTC and CTEC is large multiploid cells in peripheral blood of subjects with skin tumors. |
| first_indexed | 2024-03-08T19:00:23Z |
| format | Article |
| id | doaj.art-ee62068aa7744e688d52694fc39ab5ee |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-03-08T19:00:23Z |
| publishDate | 2023-06-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-ee62068aa7744e688d52694fc39ab5ee2023-12-28T06:53:15Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682023-06-0130319820410.3969/j.issn.1674-8468.2023.03.003Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumorsGuiyue CAI0Sirui LI1Ying LIU2Rongyi CHEN3Sujun LUO4Ping LIN5Dermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaCytointelligen, Taizhou 225300, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaCytointelligen, Taizhou 225300, ChinaObjective To investigate the clinical utility of identifying circulating tumor cells (CTC) and circulating tumorigenic endothelial cells (CTEC) in skin malignancies. Methods Subtraction enrichment-immunostaining and fluorescence in situ hybridization (SE-iFISH) were used to detect the number of CTC and CTEC in the peripheral blood of 5 patients with skin tumor (tumor group) and 14 healthy individuals (healthy controls), and the detection rate was calculated. The subtype characteristics of CTC/CTEC and the correlation between the number of CTC/CTEC and the clinical characteristics of the patients were analyzed. Results The positive rates of CTC and CTEC in the tumor group were 100%. The average CTC were (6.93±8.18) /mL and CTEC were (1.60±1.03) /mL in the peripheral blood of the tumor group. The positive rates of CTC and CTEC in the healthy controls were both 64%. The average CTC were (0.19±0.21) /mL, and CTEC were (0.30±0.33) /mL in the peripheral blood of the healthy controls. Patients with larger diameter of tumor, lymph node metastasis, and clinical stages of III and IV had higher levels of CTC and CTEC; Large CTC accounted for 88.46% (184/208) of the total tumor cells, while large CTEC accounted for 81.25% (39/48) of the total tumor cells, which mainly were ≥ pentaploid CTC and CTEC. Conclusions Using SE-iFISH, the detection rates of CTC/CTEC are high in patients with skin tumors. The amounts of CTC and CTEC are correlated with the clinical features of patients. A higher proportion of CTC and CTEC is large multiploid cells in peripheral blood of subjects with skin tumors.http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.03.003ctcctecskin tumorsubtraction enrichment-immunostaining and fluorescence in situ hybridization |
| spellingShingle | Guiyue CAI Sirui LI Ying LIU Rongyi CHEN Sujun LUO Ping LIN Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors Pifu-xingbing zhenliaoxue zazhi ctc ctec skin tumor subtraction enrichment-immunostaining and fluorescence in situ hybridization |
| title | Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors |
| title_full | Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors |
| title_fullStr | Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors |
| title_full_unstemmed | Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors |
| title_short | Significance of CTC and CTEC values in the peripheral blood of subjects with skin tumors |
| title_sort | significance of ctc and ctec values in the peripheral blood of subjects with skin tumors |
| topic | ctc ctec skin tumor subtraction enrichment-immunostaining and fluorescence in situ hybridization |
| url | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.03.003 |
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