Transcriptome Profiling of Male Adult Angiostrongylus cantonensis
Background: The pathogen of angiostrongyliasis is the parasite Angiostrongylus cantonensis, and the transcriptome profiling of the male adult was unclear. We aimed to understand how the male adults adapt, so the expression profile of A. cantonensis adult males was analyzed. Methods: In order to...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-09-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3872 |
| _version_ | 1827808079997042688 |
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| author | Guo Yue Yu Liang Cao Dong Ying Zhou Hongchang Shang Ya Qian Zhang De Long Chen Gao Qiang Jiang Bei Wu Wan Ting Lu Mingke |
| author_facet | Guo Yue Yu Liang Cao Dong Ying Zhou Hongchang Shang Ya Qian Zhang De Long Chen Gao Qiang Jiang Bei Wu Wan Ting Lu Mingke |
| author_sort | Guo Yue |
| collection | DOAJ |
| description |
Background: The pathogen of angiostrongyliasis is the parasite Angiostrongylus cantonensis, and the transcriptome profiling of the male adult was unclear. We aimed to understand how the male adults adapt, so the expression profile of A. cantonensis adult males was analyzed.
Methods: In order to improve the understanding of the transcriptome of adult males, RNA from three groups of male adult A. cantonensis was extracted and reverse transcribed to construct cDNA libraries. After sequencing, annotation of unigenes and transcripts was performed by querying the NR (Non-Redundant Protein Sequence Database), GO (Gene Ontology) and COG/ KOG (Clusters of Orthologous Groups of proteins/euKaryotic Ortholog Groups) databases.
Results: For each group of adults, 43,260,894 raw reads and 43,200,341 clean reads were obtained. After successful assembly, 87,649 unigenes and 146,895 transcripts were obtained. Annotation of the unigenes and transcripts was identical and male adults expressed a series of genes encoding proteins specific to the male gender at the adult stage, such as proteins involved in energy metabolism, energy synthesis and transport. Expression of the ribosome pathway suggests a relationship with the physical activities during the adult male stage.
Conclusion: The transcriptome analysis is a good reference to understand further the expression profile of male adult A. cantonensis.
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| format | Article |
| id | doaj.art-ee6799041c6743a1a676bef7fdf3b5d7 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-03-11T22:06:26Z |
| publishDate | 2023-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-ee6799041c6743a1a676bef7fdf3b5d72023-09-25T05:54:31ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2023-09-01183Transcriptome Profiling of Male Adult Angiostrongylus cantonensisGuo Yue0Yu Liang Cao1Dong Ying2Zhou Hongchang3Shang Ya Qian4Zhang De Long5Chen Gao Qiang6Jiang Bei7Wu Wan Ting8Lu Mingke9School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, ChinaEmergency Department, South Taihu Hospital, Gangnan Road 1566, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, ChinaSchool of Life Sciences, Xiamen University, Fujian, China Background: The pathogen of angiostrongyliasis is the parasite Angiostrongylus cantonensis, and the transcriptome profiling of the male adult was unclear. We aimed to understand how the male adults adapt, so the expression profile of A. cantonensis adult males was analyzed. Methods: In order to improve the understanding of the transcriptome of adult males, RNA from three groups of male adult A. cantonensis was extracted and reverse transcribed to construct cDNA libraries. After sequencing, annotation of unigenes and transcripts was performed by querying the NR (Non-Redundant Protein Sequence Database), GO (Gene Ontology) and COG/ KOG (Clusters of Orthologous Groups of proteins/euKaryotic Ortholog Groups) databases. Results: For each group of adults, 43,260,894 raw reads and 43,200,341 clean reads were obtained. After successful assembly, 87,649 unigenes and 146,895 transcripts were obtained. Annotation of the unigenes and transcripts was identical and male adults expressed a series of genes encoding proteins specific to the male gender at the adult stage, such as proteins involved in energy metabolism, energy synthesis and transport. Expression of the ribosome pathway suggests a relationship with the physical activities during the adult male stage. Conclusion: The transcriptome analysis is a good reference to understand further the expression profile of male adult A. cantonensis. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3872Angiostrongylus cantonensisHelminthsTranscriptome |
| spellingShingle | Guo Yue Yu Liang Cao Dong Ying Zhou Hongchang Shang Ya Qian Zhang De Long Chen Gao Qiang Jiang Bei Wu Wan Ting Lu Mingke Transcriptome Profiling of Male Adult Angiostrongylus cantonensis Iranian Journal of Parasitology Angiostrongylus cantonensis Helminths Transcriptome |
| title | Transcriptome Profiling of Male Adult Angiostrongylus cantonensis |
| title_full | Transcriptome Profiling of Male Adult Angiostrongylus cantonensis |
| title_fullStr | Transcriptome Profiling of Male Adult Angiostrongylus cantonensis |
| title_full_unstemmed | Transcriptome Profiling of Male Adult Angiostrongylus cantonensis |
| title_short | Transcriptome Profiling of Male Adult Angiostrongylus cantonensis |
| title_sort | transcriptome profiling of male adult angiostrongylus cantonensis |
| topic | Angiostrongylus cantonensis Helminths Transcriptome |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3872 |
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