The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells
Objective(s): Dendritic cells (DCs) play a critical role in activation of T cell responses. Induction of type1 T helper (Th1) immune response is essential to generate protective immunity against cutaneous leishmaniasis. The intrinsic tendency of liposomes to have interaction with antigen-presenting...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_10614_45f9a24eac05a21e7c25b7ce197a99e1.pdf |
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| author | Sara Shokooh Saremi Maryam Shahryari Razieh Ghoorchian Hamed Eshaghian Seyed Amir Jalali Amin Nikpoor Mahmoud Reza Jaafari Ali Badiee |
| author_facet | Sara Shokooh Saremi Maryam Shahryari Razieh Ghoorchian Hamed Eshaghian Seyed Amir Jalali Amin Nikpoor Mahmoud Reza Jaafari Ali Badiee |
| author_sort | Sara Shokooh Saremi |
| collection | DOAJ |
| description | Objective(s): Dendritic cells (DCs) play a critical role in activation of T cell responses. Induction of type1 T helper (Th1) immune response is essential to generate protective immunity against cutaneous leishmaniasis. The intrinsic tendency of liposomes to have interaction with antigen-presenting cells is the main rationale to utilize liposomes as antigen carriers. In the present study, the effect of lipid phase transition temperature on DCs maturation and liposome uptake by murine bone marrow derived dendritic cells and human monocyte derived dendritic cells was investigated.Materials and Methods: Two cationic liposomal formulations consisting of DOTAP and DSPC/DOTAP were prepared and contained soluble leishmania antigen. Liposomes were incubated with immature or mature DCs derived from bone marrow (BMDCs) of C57BL/6 (which are resistant to cutaneous leishmaniasis), BALB/c mice (susceptible to cutaneous leishmaniasis) or DCs derived from human monocytes (MoDCs). The expression of DCs co-stimulatory markers and liposomal uptake were evaluated by flow cytometry method. Results: DCs which were encountered to liposomes consisting of DSPC showed significantly more expression of co-stimulatory molecules in cells from both human and C57BL/6 mice but not in cells from BALB/c mice. Conclusion: It is concluded that cationic liposomes consisting of DSPC are an effective adjuvant for antigen delivery in case of MoDCs and BMDCs from C57BL/6 mice. Moreover, DCs from different origins act differently in uptake of liposomes. |
| first_indexed | 2024-04-13T09:51:29Z |
| format | Article |
| id | doaj.art-ee705d13726342c88ff888078735ac9b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T09:51:29Z |
| publishDate | 2018-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ee705d13726342c88ff888078735ac9b2022-12-22T02:51:36ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-05-0121553654510.22038/ijbms.2018.25976.639110614The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cellsSara Shokooh Saremi0Maryam Shahryari1Razieh Ghoorchian2Hamed Eshaghian3Seyed Amir Jalali4Amin Nikpoor5Mahmoud Reza Jaafari6Ali Badiee7Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Dendritic cells (DCs) play a critical role in activation of T cell responses. Induction of type1 T helper (Th1) immune response is essential to generate protective immunity against cutaneous leishmaniasis. The intrinsic tendency of liposomes to have interaction with antigen-presenting cells is the main rationale to utilize liposomes as antigen carriers. In the present study, the effect of lipid phase transition temperature on DCs maturation and liposome uptake by murine bone marrow derived dendritic cells and human monocyte derived dendritic cells was investigated.Materials and Methods: Two cationic liposomal formulations consisting of DOTAP and DSPC/DOTAP were prepared and contained soluble leishmania antigen. Liposomes were incubated with immature or mature DCs derived from bone marrow (BMDCs) of C57BL/6 (which are resistant to cutaneous leishmaniasis), BALB/c mice (susceptible to cutaneous leishmaniasis) or DCs derived from human monocytes (MoDCs). The expression of DCs co-stimulatory markers and liposomal uptake were evaluated by flow cytometry method. Results: DCs which were encountered to liposomes consisting of DSPC showed significantly more expression of co-stimulatory molecules in cells from both human and C57BL/6 mice but not in cells from BALB/c mice. Conclusion: It is concluded that cationic liposomes consisting of DSPC are an effective adjuvant for antigen delivery in case of MoDCs and BMDCs from C57BL/6 mice. Moreover, DCs from different origins act differently in uptake of liposomes.http://ijbms.mums.ac.ir/article_10614_45f9a24eac05a21e7c25b7ce197a99e1.pdfCationic liposomesDendritic cellsImmune responseLeishmaniasisPhase transition temperatureTh1 |
| spellingShingle | Sara Shokooh Saremi Maryam Shahryari Razieh Ghoorchian Hamed Eshaghian Seyed Amir Jalali Amin Nikpoor Mahmoud Reza Jaafari Ali Badiee The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells Iranian Journal of Basic Medical Sciences Cationic liposomes Dendritic cells Immune response Leishmaniasis Phase transition temperature Th1 |
| title | The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| title_full | The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| title_fullStr | The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| title_full_unstemmed | The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| title_short | The role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| title_sort | role of nanoliposome bilayer composition containing soluble leishmania antigen on maturation and activation of dendritic cells |
| topic | Cationic liposomes Dendritic cells Immune response Leishmaniasis Phase transition temperature Th1 |
| url | http://ijbms.mums.ac.ir/article_10614_45f9a24eac05a21e7c25b7ce197a99e1.pdf |
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