Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis

The conditions of enzymatic hydrolysis of thioglucosides, which are the precursors of the pungent components in Armoracia rusticana grown in China, were studied. The effects of incubation time, temperature, pH and the addition of ascorbic acid on the hydrolysis of thioglucosides were determined. The...

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Main Authors: Rong Li, Jimmy C. Yu, Zi-Tao Jiang
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2006-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/161707
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author Rong Li
Jimmy C. Yu
Zi-Tao Jiang
author_facet Rong Li
Jimmy C. Yu
Zi-Tao Jiang
author_sort Rong Li
collection DOAJ
description The conditions of enzymatic hydrolysis of thioglucosides, which are the precursors of the pungent components in Armoracia rusticana grown in China, were studied. The effects of incubation time, temperature, pH and the addition of ascorbic acid on the hydrolysis of thioglucosides were determined. The optimum hydrolytic conditions for the pungent components from thioglucosides were time, 120 min; temperature, 65 oC; pH=4.0 and ascorbic acid, 2 mg/g. The mixture of pungent components in a pale-yellow liquid and a yield of 0.85 % were isolated and analyzed by GC/MS. Nine constituents were identified, representing 92.1 % of the pungent components. The major constituents were allyl isothiocyanate (78.4 %), 3-butenyl isothiocyanate (1.5 %), 2-pentyl isothiocyanate (2.1 %) and β-phenylethyl isothiocyanate (9.4 %).
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spelling doaj.art-ee74a999681d4a26b20444651233fb1e2023-12-02T14:57:46ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062006-01-014414145Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic HydrolysisRong Li0Jimmy C. Yu1Zi-Tao Jiang2Department of Food Science and Engineering, Tianjin University of Commerce,Department of Chemistry, The Chinese University of Hong Kong,Department of Food Science and Engineering, Tianjin University of Commerce,The conditions of enzymatic hydrolysis of thioglucosides, which are the precursors of the pungent components in Armoracia rusticana grown in China, were studied. The effects of incubation time, temperature, pH and the addition of ascorbic acid on the hydrolysis of thioglucosides were determined. The optimum hydrolytic conditions for the pungent components from thioglucosides were time, 120 min; temperature, 65 oC; pH=4.0 and ascorbic acid, 2 mg/g. The mixture of pungent components in a pale-yellow liquid and a yield of 0.85 % were isolated and analyzed by GC/MS. Nine constituents were identified, representing 92.1 % of the pungent components. The major constituents were allyl isothiocyanate (78.4 %), 3-butenyl isothiocyanate (1.5 %), 2-pentyl isothiocyanate (2.1 %) and β-phenylethyl isothiocyanate (9.4 %).http://hrcak.srce.hr/file/161707Armoracia rusticanaenzymatic hydrolysisthioglucosidesisothiocyanates
spellingShingle Rong Li
Jimmy C. Yu
Zi-Tao Jiang
Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
Food Technology and Biotechnology
Armoracia rusticana
enzymatic hydrolysis
thioglucosides
isothiocyanates
title Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
title_full Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
title_fullStr Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
title_full_unstemmed Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
title_short Pungent Components from Thioglucosides in Armoracia rusticana Grown in China, Obtained by Enzymatic Hydrolysis
title_sort pungent components from thioglucosides in armoracia rusticana grown in china obtained by enzymatic hydrolysis
topic Armoracia rusticana
enzymatic hydrolysis
thioglucosides
isothiocyanates
url http://hrcak.srce.hr/file/161707
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