Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models
The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the <i>S...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/9/7/153 |
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| author | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Syla Maria Farias Ferraz Klafke Pamella Fukuda de Castilho Lucilene Finoto Viana Edson Lucas dos Santos Kelly Mari Pires de Oliveira Alexeia Barufatti |
| author_facet | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Syla Maria Farias Ferraz Klafke Pamella Fukuda de Castilho Lucilene Finoto Viana Edson Lucas dos Santos Kelly Mari Pires de Oliveira Alexeia Barufatti |
| author_sort | Luiza Flavia Veiga Francisco |
| collection | DOAJ |
| description | The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the <i>Salmonella</i> microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage; however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater. |
| first_indexed | 2024-03-09T04:44:27Z |
| format | Article |
| id | doaj.art-ee78a02de0144d77b16b5fcbbec8b142 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T04:44:27Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-ee78a02de0144d77b16b5fcbbec8b1422023-12-03T13:18:08ZengMDPI AGToxics2305-63042021-06-019715310.3390/toxics9070153Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro ModelsLuiza Flavia Veiga Francisco0Debora da Silva Baldivia1Bruno do Amaral Crispim2Syla Maria Farias Ferraz Klafke3Pamella Fukuda de Castilho4Lucilene Finoto Viana5Edson Lucas dos Santos6Kelly Mari Pires de Oliveira7Alexeia Barufatti8Faculty of Exact Sciences and Technology, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilPostgraduate Program in Health Science, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilFaculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79.804-970, Mato Grosso do Sul, BrazilThe objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the <i>Salmonella</i> microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage; however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater.https://www.mdpi.com/2305-6304/9/7/153metalscytotoxicitymicronucleuscomet assayAmes testCHO cells |
| spellingShingle | Luiza Flavia Veiga Francisco Debora da Silva Baldivia Bruno do Amaral Crispim Syla Maria Farias Ferraz Klafke Pamella Fukuda de Castilho Lucilene Finoto Viana Edson Lucas dos Santos Kelly Mari Pires de Oliveira Alexeia Barufatti Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models Toxics metals cytotoxicity micronucleus comet assay Ames test CHO cells |
| title | Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models |
| title_full | Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models |
| title_fullStr | Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models |
| title_full_unstemmed | Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models |
| title_short | Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models |
| title_sort | acute toxic and genotoxic effects of aluminum and manganese using in vitro models |
| topic | metals cytotoxicity micronucleus comet assay Ames test CHO cells |
| url | https://www.mdpi.com/2305-6304/9/7/153 |
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