miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment.
MicroRNAs (miRNAs) play a key role in different nervous system diseases. We sought to determine the role of miRNAs in isoflurane-induced learning and memory impairment in aged rats. Male Sprague-Dawley (SD) rats of 18 month were randomly assigned to control group (exposed to mock anesthesia), 2-hour...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0119336 |
| _version_ | 1818362990276116480 |
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| author | Ting Luo Shuzhou Yin Rong Shi Chengshi Xu Yun Wang Jun Cai Yun Yue Anshi Wu |
| author_facet | Ting Luo Shuzhou Yin Rong Shi Chengshi Xu Yun Wang Jun Cai Yun Yue Anshi Wu |
| author_sort | Ting Luo |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) play a key role in different nervous system diseases. We sought to determine the role of miRNAs in isoflurane-induced learning and memory impairment in aged rats. Male Sprague-Dawley (SD) rats of 18 month were randomly assigned to control group (exposed to mock anesthesia), 2-hour group and 6-hour group (exposed to 2% isoflurane for 2 and 6 hours respectively). By Morris Water Maze, 6-hour group showed impaired learning and memory ability while 2-hour group not. As shown by miRNA array, control group and 2-hour group showed a similar miRNA expression profile. And 38 miRNAs are differently expressed in 6-hour group compared to the other 2 groups, including 21 up-regulated miRNAs and 17 down-regulated miRNAs. And 4 of the differentially expressed miRNAs were validated independently by qRT-PCR. Let-7d was downregulated in 6-hour group. Additionally, we demonstrated that amyloid precursor protein (APP) was a direct target of let-7d by Fluorescent report assay. Increased expression of APP and amyloid-β (Aβ) were found in the hippocampi of 6-hour group. Downregulation of let-7d might contribute to isoflurane-induced learning and memory impairment through upregulating its target APP, and increasing the production of Aβ subsequently. |
| first_indexed | 2024-12-13T21:41:21Z |
| format | Article |
| id | doaj.art-ee7948361ed7417a90e063f9ec477056 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:41:21Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ee7948361ed7417a90e063f9ec4770562022-12-21T23:30:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011933610.1371/journal.pone.0119336miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment.Ting LuoShuzhou YinRong ShiChengshi XuYun WangJun CaiYun YueAnshi WuMicroRNAs (miRNAs) play a key role in different nervous system diseases. We sought to determine the role of miRNAs in isoflurane-induced learning and memory impairment in aged rats. Male Sprague-Dawley (SD) rats of 18 month were randomly assigned to control group (exposed to mock anesthesia), 2-hour group and 6-hour group (exposed to 2% isoflurane for 2 and 6 hours respectively). By Morris Water Maze, 6-hour group showed impaired learning and memory ability while 2-hour group not. As shown by miRNA array, control group and 2-hour group showed a similar miRNA expression profile. And 38 miRNAs are differently expressed in 6-hour group compared to the other 2 groups, including 21 up-regulated miRNAs and 17 down-regulated miRNAs. And 4 of the differentially expressed miRNAs were validated independently by qRT-PCR. Let-7d was downregulated in 6-hour group. Additionally, we demonstrated that amyloid precursor protein (APP) was a direct target of let-7d by Fluorescent report assay. Increased expression of APP and amyloid-β (Aβ) were found in the hippocampi of 6-hour group. Downregulation of let-7d might contribute to isoflurane-induced learning and memory impairment through upregulating its target APP, and increasing the production of Aβ subsequently.https://doi.org/10.1371/journal.pone.0119336 |
| spellingShingle | Ting Luo Shuzhou Yin Rong Shi Chengshi Xu Yun Wang Jun Cai Yun Yue Anshi Wu miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. PLoS ONE |
| title | miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. |
| title_full | miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. |
| title_fullStr | miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. |
| title_full_unstemmed | miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. |
| title_short | miRNA expression profile and involvement of Let-7d-APP in aged rats with isoflurane-induced learning and memory impairment. |
| title_sort | mirna expression profile and involvement of let 7d app in aged rats with isoflurane induced learning and memory impairment |
| url | https://doi.org/10.1371/journal.pone.0119336 |
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