Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis
Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2022-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200022Y.pdf |
| _version_ | 1811179351905927168 |
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| author | Yu Hui Zhang Lixia Su Rina Hu Hai Wang Zhanli |
| author_facet | Yu Hui Zhang Lixia Su Rina Hu Hai Wang Zhanli |
| author_sort | Yu Hui |
| collection | DOAJ |
| description | Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence. |
| first_indexed | 2024-04-11T06:33:17Z |
| format | Article |
| id | doaj.art-ee797b8f5e774151858805a2137a9c79 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-11T06:33:17Z |
| publishDate | 2022-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-ee797b8f5e774151858805a2137a9c792022-12-22T04:39:55ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392022-01-0174323524210.2298/ABS220613022Y0354-46642200022YAltered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysisYu Hui0Zhang Lixia1Su Rina2Hu Hai3Wang Zhanli4School of Basic Medicine, Baotou Medical College, Baotou, China + Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou, ChinaThe First Affiliated Hospital, Baotou Medical College, Baotou, ChinaThe Second Affiliated Hospital, Baotou Medical College, Baotou, ChinaSchool of Basic Medicine, Baotou Medical College, Baotou , ChinaInner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou, ChinaKlebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence.http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200022Y.pdfklebsiella pneumoniaevirulenceproteomicsmetabolic pathway |
| spellingShingle | Yu Hui Zhang Lixia Su Rina Hu Hai Wang Zhanli Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis Archives of Biological Sciences klebsiella pneumoniae virulence proteomics metabolic pathway |
| title | Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis |
| title_full | Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis |
| title_fullStr | Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis |
| title_full_unstemmed | Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis |
| title_short | Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis |
| title_sort | altered metabolic pathways in classic and hypervirulent klebsiella pneumoniae isolates revealed by proteomics analysis |
| topic | klebsiella pneumoniae virulence proteomics metabolic pathway |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200022Y.pdf |
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