Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85
Objectives: Heteroresistance is a phenomenon in which there are various responses to antibiotics from bacterial cells within the same population. Here, we isolated and characterised an imipenem heteroresistant Acinetobacter baumannii strain (HRAB-85). Methods: The genome of strain HRAB-85 was comple...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-09-01
|
| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971217301832 |
| _version_ | 1811223613487972352 |
|---|---|
| author | Puyuan Li Yong Huang Lan Yu Yannan Liu Wenkai Niu Dayang Zou Huiying Liu Jing Zheng Xiuyun Yin Jing Yuan Xin Yuan Changqing Bai |
| author_facet | Puyuan Li Yong Huang Lan Yu Yannan Liu Wenkai Niu Dayang Zou Huiying Liu Jing Zheng Xiuyun Yin Jing Yuan Xin Yuan Changqing Bai |
| author_sort | Puyuan Li |
| collection | DOAJ |
| description | Objectives: Heteroresistance is a phenomenon in which there are various responses to antibiotics from bacterial cells within the same population. Here, we isolated and characterised an imipenem heteroresistant Acinetobacter baumannii strain (HRAB-85).
Methods: The genome of strain HRAB-85 was completely sequenced and analysed to understand its antibiotic resistance mechanisms. Population analysis and multilocus sequence typing were performed.
Results: Subpopulations grew in the presence of imipenem at concentrations of up to 64 μg/mL, and the strain was found to belong to ST208. The total length of strain HRAB-85 was 4,098,585 bp with a GC content of 39.98%. The genome harboured at least four insertion sequences: the common ISAba1, ISAba22, ISAba24, and newly reported ISAba26. Additionally, 19 antibiotic-resistance genes against eight classes of antimicrobial agents were found, and 11 genomic islands (GIs) were identified. Among them, GI3, GI10, and GI11 contained many ISs and antibiotic-resistance determinants.
Conclusions: The existence of imipenem heteroresistant phenotypes in A. baumannii was substantiated in this hospital, and imipenem pressure, which could induce imipenem-heteroresistant subpopulations, may select for highly resistant strains. The complete genome sequencing and bioinformatics analysis of HRAB-85 could improve our understanding of the epidemiology and resistance mechanisms of carbapenem-heteroresistant A. baumannii. |
| first_indexed | 2024-04-12T08:35:46Z |
| format | Article |
| id | doaj.art-ee7c6d124cd444bbaecbc620e1050fbb |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-04-12T08:35:46Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-ee7c6d124cd444bbaecbc620e1050fbb2022-12-22T03:40:01ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112017-09-0162C9410110.1016/j.ijid.2017.07.005Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85Puyuan Li0Yong Huang1Lan Yu2Yannan Liu3Wenkai Niu4Dayang Zou5Huiying Liu6Jing Zheng7Xiuyun Yin8Jing Yuan9Xin Yuan10Changqing Bai11Department of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaDepartment of Gastroenterology, Navy General Hospital, 6 Fucheng Road, Beijing 100048, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaDepartment of Clinical Laboratory, 307th Hospital of PLA, Beijing 100071, ChinaInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaDepartment of Respiratory and Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, ChinaObjectives: Heteroresistance is a phenomenon in which there are various responses to antibiotics from bacterial cells within the same population. Here, we isolated and characterised an imipenem heteroresistant Acinetobacter baumannii strain (HRAB-85). Methods: The genome of strain HRAB-85 was completely sequenced and analysed to understand its antibiotic resistance mechanisms. Population analysis and multilocus sequence typing were performed. Results: Subpopulations grew in the presence of imipenem at concentrations of up to 64 μg/mL, and the strain was found to belong to ST208. The total length of strain HRAB-85 was 4,098,585 bp with a GC content of 39.98%. The genome harboured at least four insertion sequences: the common ISAba1, ISAba22, ISAba24, and newly reported ISAba26. Additionally, 19 antibiotic-resistance genes against eight classes of antimicrobial agents were found, and 11 genomic islands (GIs) were identified. Among them, GI3, GI10, and GI11 contained many ISs and antibiotic-resistance determinants. Conclusions: The existence of imipenem heteroresistant phenotypes in A. baumannii was substantiated in this hospital, and imipenem pressure, which could induce imipenem-heteroresistant subpopulations, may select for highly resistant strains. The complete genome sequencing and bioinformatics analysis of HRAB-85 could improve our understanding of the epidemiology and resistance mechanisms of carbapenem-heteroresistant A. baumannii.http://www.sciencedirect.com/science/article/pii/S1201971217301832Acinetobacter baumanniiheteroresistancecarbapenemgenomic islandinsertion sequence |
| spellingShingle | Puyuan Li Yong Huang Lan Yu Yannan Liu Wenkai Niu Dayang Zou Huiying Liu Jing Zheng Xiuyun Yin Jing Yuan Xin Yuan Changqing Bai Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 International Journal of Infectious Diseases Acinetobacter baumannii heteroresistance carbapenem genomic island insertion sequence |
| title | Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 |
| title_full | Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 |
| title_fullStr | Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 |
| title_full_unstemmed | Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 |
| title_short | Isolation and Whole-genome Sequence Analysis of the Imipenem Heteroresistant Acinetobacter baumannii Clinical Isolate HRAB-85 |
| title_sort | isolation and whole genome sequence analysis of the imipenem heteroresistant acinetobacter baumannii clinical isolate hrab 85 |
| topic | Acinetobacter baumannii heteroresistance carbapenem genomic island insertion sequence |
| url | http://www.sciencedirect.com/science/article/pii/S1201971217301832 |
| work_keys_str_mv | AT puyuanli isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT yonghuang isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT lanyu isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT yannanliu isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT wenkainiu isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT dayangzou isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT huiyingliu isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT jingzheng isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT xiuyunyin isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT jingyuan isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT xinyuan isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 AT changqingbai isolationandwholegenomesequenceanalysisoftheimipenemheteroresistantacinetobacterbaumanniiclinicalisolatehrab85 |