Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra
Abstract Background Brassica nigra is a plant of Brassicaceae family, which possesses numerous medicinal values. Our present study is intended to assess the potential in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic properties of MCE of B. nigra flowers. MCE was fractioned for...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2020-10-01
|
| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43094-020-00099-x |
| _version_ | 1819114643770048512 |
|---|---|
| author | Mohammad Sarowar Uddin Md. Shalahuddin Millat Mohammad Safiqul Islam Md. Saddam Hussain Md. Giash Uddin Shafayet Ahmed Siddiqui Mahmuda Ferdous |
| author_facet | Mohammad Sarowar Uddin Md. Shalahuddin Millat Mohammad Safiqul Islam Md. Saddam Hussain Md. Giash Uddin Shafayet Ahmed Siddiqui Mahmuda Ferdous |
| author_sort | Mohammad Sarowar Uddin |
| collection | DOAJ |
| description | Abstract Background Brassica nigra is a plant of Brassicaceae family, which possesses numerous medicinal values. Our present study is intended to assess the potential in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic properties of MCE of B. nigra flowers. MCE was fractioned for separating the compound on the basis of polarity by using chloroform, n-hexane and ethyl acetate solvent. Thrombolytic and anthelminthic activities were explained by collecting human erythrocytes and earthworms as test models, respectively. Anxiolytic activity was evaluated by elevated plus maze and hole board models while cytotoxic test was conducted through brine shrimp lethality bioassay. Results MCE revealed the presence of alkaloids, flavonoids, tannin, diterpenes, glycosides, carbohydrates, phenols, fixed oils and fat. In case of thrombolytic test, the MCE, CSF, ASF and n-HSF had produced maximum clot lysis activity at 5 and 10 mg/ml dose conditions. Two different concentrations (10 and 20 mg/ml) of MCE and its fractions showed significant (p < 0.05) anthelminthic activities in a dose-dependent manner. Significant anxiolytic activity was observed for all fractions which was comparable to the standard drug diazepam (p < 0.05). Again, the cytotoxic screening also presented good potentials for all fractions. Conclusion From the findings of present study, we can conclude that MCE of B. nigra flowers and its fraction possess significant anxiolytic, anthelmintic, anticancer and thrombolytic properties which may be a good candidate for treating these diseases through the determination of bio-active lead compounds. |
| first_indexed | 2024-12-22T04:48:34Z |
| format | Article |
| id | doaj.art-ee8091b4f9cb4e7bb27b26ccbb9ab075 |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-12-22T04:48:34Z |
| publishDate | 2020-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-ee8091b4f9cb4e7bb27b26ccbb9ab0752022-12-21T18:38:32ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532020-10-01611910.1186/s43094-020-00099-xExploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigraMohammad Sarowar Uddin0Md. Shalahuddin Millat1Mohammad Safiqul Islam2Md. Saddam Hussain3Md. Giash Uddin4Shafayet Ahmed Siddiqui5Mahmuda Ferdous6Department of Pharmacy, Noakhali Science & Technology UniversityDepartment of Pharmacy, Noakhali Science & Technology UniversityDepartment of Pharmacy, Noakhali Science & Technology UniversityDepartment of Pharmacy, Noakhali Science & Technology UniversityDepartment of Pharmacy, University of ChittagongDepartment of Pharmacy, Atish Dipankar University of Science and TechnologyDepartment of Pharmacy, Noakhali Science & Technology UniversityAbstract Background Brassica nigra is a plant of Brassicaceae family, which possesses numerous medicinal values. Our present study is intended to assess the potential in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic properties of MCE of B. nigra flowers. MCE was fractioned for separating the compound on the basis of polarity by using chloroform, n-hexane and ethyl acetate solvent. Thrombolytic and anthelminthic activities were explained by collecting human erythrocytes and earthworms as test models, respectively. Anxiolytic activity was evaluated by elevated plus maze and hole board models while cytotoxic test was conducted through brine shrimp lethality bioassay. Results MCE revealed the presence of alkaloids, flavonoids, tannin, diterpenes, glycosides, carbohydrates, phenols, fixed oils and fat. In case of thrombolytic test, the MCE, CSF, ASF and n-HSF had produced maximum clot lysis activity at 5 and 10 mg/ml dose conditions. Two different concentrations (10 and 20 mg/ml) of MCE and its fractions showed significant (p < 0.05) anthelminthic activities in a dose-dependent manner. Significant anxiolytic activity was observed for all fractions which was comparable to the standard drug diazepam (p < 0.05). Again, the cytotoxic screening also presented good potentials for all fractions. Conclusion From the findings of present study, we can conclude that MCE of B. nigra flowers and its fraction possess significant anxiolytic, anthelmintic, anticancer and thrombolytic properties which may be a good candidate for treating these diseases through the determination of bio-active lead compounds.http://link.springer.com/article/10.1186/s43094-020-00099-xBrassica nigraAnxiolyticAnthelminticThrombolyticCytotoxic activity |
| spellingShingle | Mohammad Sarowar Uddin Md. Shalahuddin Millat Mohammad Safiqul Islam Md. Saddam Hussain Md. Giash Uddin Shafayet Ahmed Siddiqui Mahmuda Ferdous Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra Future Journal of Pharmaceutical Sciences Brassica nigra Anxiolytic Anthelmintic Thrombolytic Cytotoxic activity |
| title | Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra |
| title_full | Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra |
| title_fullStr | Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra |
| title_full_unstemmed | Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra |
| title_short | Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra |
| title_sort | exploration of in vitro thrombolytic anthelminthic cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of brassica nigra |
| topic | Brassica nigra Anxiolytic Anthelmintic Thrombolytic Cytotoxic activity |
| url | http://link.springer.com/article/10.1186/s43094-020-00099-x |
| work_keys_str_mv | AT mohammadsarowaruddin explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT mdshalahuddinmillat explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT mohammadsafiqulislam explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT mdsaddamhussain explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT mdgiashuddin explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT shafayetahmedsiddiqui explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra AT mahmudaferdous explorationofinvitrothrombolyticanthelminthiccytotoxicandinvivoanxiolyticpotentialswithphytochemicalscreeningofflowersofbrassicanigra |