Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells
Abstract Objectives The aim of this study was whether quercetin induces cell death by caspase and MAPK signaling pathway in EGFR mutant lung cancer cells Methods PC-9 cells, EGFR mutant lung cancer cells, were treated various times and concentrations of quercetin and harvested and measured using MTT...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Kosin University College of Medicine
2016-06-01
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| Series: | Kosin Medical Journal |
| Subjects: | |
| Online Access: | http://www.kosinmedj.org/upload/pdf/ksmc031-01-04.pdf |
| _version_ | 1811255755636998144 |
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| author | Eun Jin Lim Jeunghoon Heo Young-Ho Kim |
| author_facet | Eun Jin Lim Jeunghoon Heo Young-Ho Kim |
| author_sort | Eun Jin Lim |
| collection | DOAJ |
| description | Abstract Objectives The aim of this study was whether quercetin induces cell death by caspase and MAPK signaling pathway in EGFR mutant lung cancer cells Methods PC-9 cells, EGFR mutant lung cancer cells, were treated various times and concentrations of quercetin and harvested and measured using MTT assay, DNA fragmentation, Western blotting, and FACS analysis. Results Treatment with quercetin in PC-9 cells resulted in inhibition of cell growth through apoptosis. Quercetin-induced apoptosis was associated with caspase-dependent manner. Quercetin also significantly increased levels of phosphor-p38 and decreased levels of phosphor-ERK, indicating that quercetin induces p38 MAPK signaling pathway in PC-9 cells. Quecetin treatment also generated the release of cytochrome c in PC-9 cells; however, pretreatment with rotenone or z-LEHD-fmk, significantly attenuated quercetin-induced apoptosis. Conclusions Our data indicate that quercetin exhibits EGFR mutant lung cancer effects through apoptosis by caspase dependent and mitochondrial pathway. |
| first_indexed | 2024-04-12T17:29:39Z |
| format | Article |
| id | doaj.art-ee81f3b02add40379d4be028a8a10a09 |
| institution | Directory Open Access Journal |
| issn | 2005-9531 2586-7024 |
| language | English |
| last_indexed | 2024-04-12T17:29:39Z |
| publishDate | 2016-06-01 |
| publisher | Kosin University College of Medicine |
| record_format | Article |
| series | Kosin Medical Journal |
| spelling | doaj.art-ee81f3b02add40379d4be028a8a10a092022-12-22T03:23:11ZengKosin University College of MedicineKosin Medical Journal2005-95312586-70242016-06-01311304010.7180/kmj.2016.31.1.301165Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cellsEun Jin Lim0Jeunghoon Heo1Young-Ho Kim2Department of Molecular Biology and Immunology, College of Medicine, Kosin University, Busan, KoreaDepartment of Molecular Biology and Immunology, College of Medicine, Kosin University, Busan, KoreaDepartment of Molecular Biology and Immunology, College of Medicine, Kosin University, Busan, KoreaAbstract Objectives The aim of this study was whether quercetin induces cell death by caspase and MAPK signaling pathway in EGFR mutant lung cancer cells Methods PC-9 cells, EGFR mutant lung cancer cells, were treated various times and concentrations of quercetin and harvested and measured using MTT assay, DNA fragmentation, Western blotting, and FACS analysis. Results Treatment with quercetin in PC-9 cells resulted in inhibition of cell growth through apoptosis. Quercetin-induced apoptosis was associated with caspase-dependent manner. Quercetin also significantly increased levels of phosphor-p38 and decreased levels of phosphor-ERK, indicating that quercetin induces p38 MAPK signaling pathway in PC-9 cells. Quecetin treatment also generated the release of cytochrome c in PC-9 cells; however, pretreatment with rotenone or z-LEHD-fmk, significantly attenuated quercetin-induced apoptosis. Conclusions Our data indicate that quercetin exhibits EGFR mutant lung cancer effects through apoptosis by caspase dependent and mitochondrial pathway.http://www.kosinmedj.org/upload/pdf/ksmc031-01-04.pdfegfr mutantlung cancermapkmitochondriaquercetin |
| spellingShingle | Eun Jin Lim Jeunghoon Heo Young-Ho Kim Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells Kosin Medical Journal egfr mutant lung cancer mapk mitochondria quercetin |
| title | Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells |
| title_full | Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells |
| title_fullStr | Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells |
| title_full_unstemmed | Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells |
| title_short | Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells |
| title_sort | quercetin induces cell death by caspase dependent and p38 mapk pathway in egfr mutant lung cancer cells |
| topic | egfr mutant lung cancer mapk mitochondria quercetin |
| url | http://www.kosinmedj.org/upload/pdf/ksmc031-01-04.pdf |
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